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The map label for this gene is oprM [H]

Identifier: 116689762

GI number: 116689762

Start: 1935711

End: 1937279

Strand: Direct

Name: oprM [H]

Synonym: Bcen2424_1741

Alternate gene names: 116689762

Gene position: 1935711-1937279 (Clockwise)

Preceding gene: 116689761

Following gene: 116689763

Centisome position: 55.56

GC content: 72.85

Gene sequence:

>1569_bases
ATGGATCGATCGATGACCCTGAAAACGAAAGCCGTGCGCGCGCTCACGGTGGCGAGCCTGGCCGGCCCGCTCGCGGGCTG
CGCGTGGTTCGCGCCGAGCGGCGAGCCGCCCGCGATGCCGTCGCCCGCGCACTACGGCGCCGCGCCGCAAGTCGAGCAGA
CCGTGTCCGCGCACGGCGTCGCGCAGCAGTTCGAGGTCGGCGCGCAGCCGGTGCCGGACTGGTGGAAACAGTACCGTTCC
GACGCGCTGAACGCGCTCGTCGACGAAGGGCTGCGCAACAGCCCGACATTGAGTGCGGCGTCGCATTCGCTGGATGCCGC
GCGCGAACAGTTACGCGGTCAGATCGGCAGCTCGATGCTGCCGTCGATCGACGCGGGCGGCCAGGCCACGCGCCAGCGCG
CGCTCGGCGTGCCGATTCCCGCGCTCGGCGCGCCGACGCTGCTGTACGACACGTTCGTCGGGCAACTGCAGGCGAGCTAC
ACGGTCGACCTGTTCGGCGTGTCGCGCTTCGCGAACCGCGCGCTCGCGAAACGCGTCGACGTCAGCGCGTTCCAGCTCGA
ATCCGCGCGGCGCGCGCTGGCCGCGAACATCGTCACCGCATCGATCACCGTGTCGGTGCTCAATGCGCAGATCGACACGA
CCGAGCGGCTCGTCGCGCTCGCGAACGACCAGGCGCACGACGCGGAGCGCCGCCATGCGCTCGGTTCGGCCTCGCGCAGC
GACGCGTTGAACGCCCGGCAAAGCGCCGACACGTTCGCCGCGAGCCTGCCCGCACTGCGCCAGCAGCGCGACTCGGCGCG
TCACGCGCTGGCGGTGCTGGTCGGCCGCACGCCCGACCGGCCGCCGGCCGATCTCGCGCTCACCGATCTGCACCTGCCCG
AGCAGGTGCCCGTCGTCGTGCCGTCGGACCTGCTGCAAAGCCGCCCCGACATCCAGGCCGCCGACGCGGGGCTGAAGGCC
GCCGCCGCCGAAGTCGGCCTCGCGACCGCGCAGATGTTTCCGCAACTGTCGCTGTCGGCGGCGATGGGCAAAGGCGGCTT
CAGCTGGCCGGCGATGCTGTCGGGCGCCGGCGCGATCTGGAACGTCGGCGCGTCGCTGAGCCAGCCGATCTTCCATGGCG
GCGCGCTGCTCGCCCAGCGCCGCGCGGCGAAAGCGACCTACGAGGCCGCGGTCGACCAGTACAAGCAGGCGGTGCTCGGC
GCGTTCCAGAACGTCGCCGATTCGCTTGCGGCGCTCGAGCACGACGCGGAGGCGCTCGATGCGTCGTCGCGCGCCGCGCT
GTCCGCACGGGGCGCGTACGACGACGCGGCCGCTCGCGTGCGGCTCGGTGCGTTGCCGCCGTCGGCGGCGCGCGCGAGCG
AGCTGCAGTACCGCAATGCGCGGCTCGACGAGATTCGCGCGACCGGCGCACGGTTCGCGGATACCGCGCGGCTTTACCAG
GCGATGGGCACACCGCCGGCCGACACCGGCGACAAGTCCGGTAAAACCGGCCACGCGGCCGGCGACGGCACAGCCGGCAC
GCAAGCACGCGCCGCCACGCCCGACGCCGCCGTCCCTTCGGCGCAATAA

Upstream 100 bases:

>100_bases
TGTACGCGACGTGGTTCCGCGTGAAGCGCGACGAACGCGACCCGCAGGACGGCCCGCCGCCAGGCGGCAGCATGCCGGCC
GCGCCGACGGGAGCCTGACC

Downstream 100 bases:

>100_bases
CCAGCCCCCTTCGATCGGATGCGCGTGCGGATTCCCGCCACGCGCGTCCGATCGCCGCTCTTTCGCTTGACCCTCACGTA
GCGTAACGTTCGAGACTCCA

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MDRSMTLKTKAVRALTVASLAGPLAGCAWFAPSGEPPAMPSPAHYGAAPQVEQTVSAHGVAQQFEVGAQPVPDWWKQYRS
DALNALVDEGLRNSPTLSAASHSLDAAREQLRGQIGSSMLPSIDAGGQATRQRALGVPIPALGAPTLLYDTFVGQLQASY
TVDLFGVSRFANRALAKRVDVSAFQLESARRALAANIVTASITVSVLNAQIDTTERLVALANDQAHDAERRHALGSASRS
DALNARQSADTFAASLPALRQQRDSARHALAVLVGRTPDRPPADLALTDLHLPEQVPVVVPSDLLQSRPDIQAADAGLKA
AAAEVGLATAQMFPQLSLSAAMGKGGFSWPAMLSGAGAIWNVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLG
AFQNVADSLAALEHDAEALDASSRAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDEIRATGARFADTARLYQ
AMGTPPADTGDKSGKTGHAAGDGTAGTQARAATPDAAVPSAQ

Sequences:

>Translated_522_residues
MDRSMTLKTKAVRALTVASLAGPLAGCAWFAPSGEPPAMPSPAHYGAAPQVEQTVSAHGVAQQFEVGAQPVPDWWKQYRS
DALNALVDEGLRNSPTLSAASHSLDAAREQLRGQIGSSMLPSIDAGGQATRQRALGVPIPALGAPTLLYDTFVGQLQASY
TVDLFGVSRFANRALAKRVDVSAFQLESARRALAANIVTASITVSVLNAQIDTTERLVALANDQAHDAERRHALGSASRS
DALNARQSADTFAASLPALRQQRDSARHALAVLVGRTPDRPPADLALTDLHLPEQVPVVVPSDLLQSRPDIQAADAGLKA
AAAEVGLATAQMFPQLSLSAAMGKGGFSWPAMLSGAGAIWNVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLG
AFQNVADSLAALEHDAEALDASSRAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDEIRATGARFADTARLYQ
AMGTPPADTGDKSGKTGHAAGDGTAGTQARAATPDAAVPSAQ
>Mature_522_residues
MDRSMTLKTKAVRALTVASLAGPLAGCAWFAPSGEPPAMPSPAHYGAAPQVEQTVSAHGVAQQFEVGAQPVPDWWKQYRS
DALNALVDEGLRNSPTLSAASHSLDAAREQLRGQIGSSMLPSIDAGGQATRQRALGVPIPALGAPTLLYDTFVGQLQASY
TVDLFGVSRFANRALAKRVDVSAFQLESARRALAANIVTASITVSVLNAQIDTTERLVALANDQAHDAERRHALGSASRS
DALNARQSADTFAASLPALRQQRDSARHALAVLVGRTPDRPPADLALTDLHLPEQVPVVVPSDLLQSRPDIQAADAGLKA
AAAEVGLATAQMFPQLSLSAAMGKGGFSWPAMLSGAGAIWNVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLG
AFQNVADSLAALEHDAEALDASSRAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDEIRATGARFADTARLYQ
AMGTPPADTGDKSGKTGHAAGDGTAGTQARAATPDAAVPSAQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=468, Percent_Identity=25.2136752136752, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI2367348, Length=449, Percent_Identity=22.9398663697105, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54143; Mature: 54143

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRSMTLKTKAVRALTVASLAGPLAGCAWFAPSGEPPAMPSPAHYGAAPQVEQTVSAHGV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AQQFEVGAQPVPDWWKQYRSDALNALVDEGLRNSPTLSAASHSLDAAREQLRGQIGSSML
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PSIDAGGQATRQRALGVPIPALGAPTLLYDTFVGQLQASYTVDLFGVSRFANRALAKRVD
CCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHC
VSAFQLESARRALAANIVTASITVSVLNAQIDTTERLVALANDQAHDAERRHALGSASRS
HHHHHHHHHHHHHHHHHHHHHHEEEHHHCHHCHHHHHHHHCCCCHHHHHHHHHHCCCCHH
DALNARQSADTFAASLPALRQQRDSARHALAVLVGRTPDRPPADLALTDLHLPEQVPVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCEEC
PSDLLQSRPDIQAADAGLKAAAAEVGLATAQMFPQLSLSAAMGKGGFSWPAMLSGAGAIW
CHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHH
NVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLGAFQNVADSLAALEHDAEALD
HCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASSRAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDEIRATGARFADTARLYQ
CCHHHHHHHCCCCCHHHHHEEECCCCCCHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHH
AMGTPPADTGDKSGKTGHAAGDGTAGTQARAATPDAAVPSAQ
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDRSMTLKTKAVRALTVASLAGPLAGCAWFAPSGEPPAMPSPAHYGAAPQVEQTVSAHGV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AQQFEVGAQPVPDWWKQYRSDALNALVDEGLRNSPTLSAASHSLDAAREQLRGQIGSSML
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PSIDAGGQATRQRALGVPIPALGAPTLLYDTFVGQLQASYTVDLFGVSRFANRALAKRVD
CCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHC
VSAFQLESARRALAANIVTASITVSVLNAQIDTTERLVALANDQAHDAERRHALGSASRS
HHHHHHHHHHHHHHHHHHHHHHEEEHHHCHHCHHHHHHHHCCCCHHHHHHHHHHCCCCHH
DALNARQSADTFAASLPALRQQRDSARHALAVLVGRTPDRPPADLALTDLHLPEQVPVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCEEC
PSDLLQSRPDIQAADAGLKAAAAEVGLATAQMFPQLSLSAAMGKGGFSWPAMLSGAGAIW
CHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHH
NVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLGAFQNVADSLAALEHDAEALD
HCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASSRAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDEIRATGARFADTARLYQ
CCHHHHHHHCCCCCHHHHHEEECCCCCCHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHH
AMGTPPADTGDKSGKTGHAAGDGTAGTQARAATPDAAVPSAQ
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]