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The map label for this gene is nolF [H]

Identifier: 116689760

GI number: 116689760

Start: 1931453

End: 1932562

Strand: Direct

Name: nolF [H]

Synonym: Bcen2424_1739

Alternate gene names: 116689760

Gene position: 1931453-1932562 (Clockwise)

Preceding gene: 116689758

Following gene: 116689761

Centisome position: 55.44

GC content: 71.8

Gene sequence:

>1110_bases
GTGAATCGCTCCGGTTCCCGCGCCGCGCTGCTGATCGGCGCCGCGCTCGTCCTTGCCGCCTGTCATCCGAAAGAATCCGC
ACCACCCGCCCCGCGCCCCGTCGTCGCGCTGCCCGCGCGGGCCGACACCGCCGCGGTCGCGCGCACGCTGCCCGGCGACA
TCCAGCCGCGCTATGCAACGCCGCTGTCGTTCCGCATCGCCGGCAAGATCATCGAGCGCAAGGTTCGGCTCGGCGACACG
GTGAAAGTCGGCCAGGTCGTCGCGCTCCTCGATCCGTCCGACGTCGAGAAGAACGCCGCGAGTGCGCAGGCGCAATTCGA
TGCCGCGTCGCACAGCCTCGCGTTCGCGAAGCAACAGCTCGATCGCGATCGTGCGCAGGCCCGCGAAAACCTGATCGCGA
CCGCGCAGCTCGAACAGACCCAGAACAGCTACACGTCGGCGCTCGCGCAGCGCGACCAGGCGCAGCAGCAGCTCGCGCTC
GCGAAGAACCAGCTGCGCTACGCGACGCTCGTCGCCGATCATGCGGGCACCATCACCGCCGAACAGGCCGATACCGGCCA
GAACGTGTCGGCCGGCCAGCCGGTCTACCAGCTCGCGTGGTCGGGCGACGTCGACGTGGTCAGCGACGTGCCCGAGGCCG
CGCTCGCGTCGCTCGCGCCCGGTCATGCGGCCACCGTCACGCTGCCGTCGCTGCCGGGCCGACAATTCGCCGCGAAGGTG
CGCGAAATCGCGCCGGCCGCCGATCCGCAAAGCCGCACCTACCGCGTGAAGCTCACGCTCGGCACGCCCGATCCGGCGAT
CCGGCTCGGCATGACCGCGGACGTCGCGTTCGACGGCGCGCCGGCCGCCGGCGACGCGCAGCCGATCACGCTGCCCGCGA
CCGCGCTGTTCCACGACGGCCCGCATCCGGCCGTGTGGATCGTGCGCACCAGGGACGACACGCTCGAGTTGCGCCGCGTC
GACGTCGCGCGCTTCAACGAGCGCACCGTCACCGTCTCGCACGGGCTGCAGCCGGGCGAGCGCGTCGTGCTGCAGGGCGT
GCATACGGTCAGCGCGGGCGAGAAGGTGCGCGCGATCGCGCCGCTGCATCCGGAGGACTTCGCATCGTGA

Upstream 100 bases:

>100_bases
TGTCGTTCGTCTGTCCGTGTGCGCCGCTGCCGGCGCCGCTTGGGGCAAGCGCCTGCCGCGCTTGCCGGCTTCGTTCCGCT
CCACCTCACCTGGGGGTTTC

Downstream 100 bases:

>100_bases
GCGTCTCCCACGAAGAAGGCCGCTTCAACCTGTCCGCGTGGGCGCTGCGCCACCAGGCGCTGGTCGTCTACCTGATCGCG
CTCGCGACGCTCGCGGGCAT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNRSGSRAALLIGAALVLAACHPKESAPPAPRPVVALPARADTAAVARTLPGDIQPRYATPLSFRIAGKIIERKVRLGDT
VKVGQVVALLDPSDVEKNAASAQAQFDAASHSLAFAKQQLDRDRAQARENLIATAQLEQTQNSYTSALAQRDQAQQQLAL
AKNQLRYATLVADHAGTITAEQADTGQNVSAGQPVYQLAWSGDVDVVSDVPEAALASLAPGHAATVTLPSLPGRQFAAKV
REIAPAADPQSRTYRVKLTLGTPDPAIRLGMTADVAFDGAPAAGDAQPITLPATALFHDGPHPAVWIVRTRDDTLELRRV
DVARFNERTVTVSHGLQPGERVVLQGVHTVSAGEKVRAIAPLHPEDFAS

Sequences:

>Translated_369_residues
MNRSGSRAALLIGAALVLAACHPKESAPPAPRPVVALPARADTAAVARTLPGDIQPRYATPLSFRIAGKIIERKVRLGDT
VKVGQVVALLDPSDVEKNAASAQAQFDAASHSLAFAKQQLDRDRAQARENLIATAQLEQTQNSYTSALAQRDQAQQQLAL
AKNQLRYATLVADHAGTITAEQADTGQNVSAGQPVYQLAWSGDVDVVSDVPEAALASLAPGHAATVTLPSLPGRQFAAKV
REIAPAADPQSRTYRVKLTLGTPDPAIRLGMTADVAFDGAPAAGDAQPITLPATALFHDGPHPAVWIVRTRDDTLELRRV
DVARFNERTVTVSHGLQPGERVVLQGVHTVSAGEKVRAIAPLHPEDFAS
>Mature_369_residues
MNRSGSRAALLIGAALVLAACHPKESAPPAPRPVVALPARADTAAVARTLPGDIQPRYATPLSFRIAGKIIERKVRLGDT
VKVGQVVALLDPSDVEKNAASAQAQFDAASHSLAFAKQQLDRDRAQARENLIATAQLEQTQNSYTSALAQRDQAQQQLAL
AKNQLRYATLVADHAGTITAEQADTGQNVSAGQPVYQLAWSGDVDVVSDVPEAALASLAPGHAATVTLPSLPGRQFAAKV
REIAPAADPQSRTYRVKLTLGTPDPAIRLGMTADVAFDGAPAAGDAQPITLPATALFHDGPHPAVWIVRTRDDTLELRRV
DVARFNERTVTVSHGLQPGERVVLQGVHTVSAGEKVRAIAPLHPEDFAS

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Attached To The Inner Membrane By A Lipid Anchor [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=384, Percent_Identity=22.1354166666667, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87081796, Length=301, Percent_Identity=25.5813953488372, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082036, Length=327, Percent_Identity=24.4648318042813, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38966; Mature: 38966

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSGSRAALLIGAALVLAACHPKESAPPAPRPVVALPARADTAAVARTLPGDIQPRYAT
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCC
PLSFRIAGKIIERKVRLGDTVKVGQVVALLDPSDVEKNAASAQAQFDAASHSLAFAKQQL
CEEHHHHHHHHHHHHHCCCCEEECEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DRDRAQARENLIATAQLEQTQNSYTSALAQRDQAQQQLALAKNQLRYATLVADHAGTITA
HHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEE
EQADTGQNVSAGQPVYQLAWSGDVDVVSDVPEAALASLAPGHAATVTLPSLPGRQFAAKV
CCCCCCCCCCCCCCEEEEEECCCCHHHHCCCHHHHHHCCCCCEEEEEECCCCCHHHHHHH
REIAPAADPQSRTYRVKLTLGTPDPAIRLGMTADVAFDGAPAAGDAQPITLPATALFHDG
HHHCCCCCCCCCEEEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCEEECHHEEECCC
PHPAVWIVRTRDDTLELRRVDVARFNERTVTVSHGLQPGERVVLQGVHTVSAGEKVRAIA
CCCEEEEEEECCCCEEHHEEHHHHCCCEEEEEECCCCCCHHHHEECCEECCCCCCEEEEC
PLHPEDFAS
CCCCHHCCC
>Mature Secondary Structure
MNRSGSRAALLIGAALVLAACHPKESAPPAPRPVVALPARADTAAVARTLPGDIQPRYAT
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCC
PLSFRIAGKIIERKVRLGDTVKVGQVVALLDPSDVEKNAASAQAQFDAASHSLAFAKQQL
CEEHHHHHHHHHHHHHCCCCEEECEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DRDRAQARENLIATAQLEQTQNSYTSALAQRDQAQQQLALAKNQLRYATLVADHAGTITA
HHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEE
EQADTGQNVSAGQPVYQLAWSGDVDVVSDVPEAALASLAPGHAATVTLPSLPGRQFAAKV
CCCCCCCCCCCCCCEEEEEECCCCHHHHCCCHHHHHHCCCCCEEEEEECCCCCHHHHHHH
REIAPAADPQSRTYRVKLTLGTPDPAIRLGMTADVAFDGAPAAGDAQPITLPATALFHDG
HHHCCCCCCCCCEEEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCEEECHHEEECCC
PHPAVWIVRTRDDTLELRRVDVARFNERTVTVSHGLQPGERVVLQGVHTVSAGEKVRAIA
CCCEEEEEEECCCCEEHHEEHHHHCCCEEEEEECCCCCCHHHHEECCEECCCCCCEEEEC
PLHPEDFAS
CCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]