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The map label for this gene is cobB [H]

Identifier: 116689673

GI number: 116689673

Start: 1833610

End: 1834920

Strand: Reverse

Name: cobB [H]

Synonym: Bcen2424_1651

Alternate gene names: 116689673

Gene position: 1834920-1833610 (Counterclockwise)

Preceding gene: 116689674

Following gene: 116689670

Centisome position: 52.67

GC content: 69.95

Gene sequence:

>1311_bases
ATGCCGGCCTGCCCCGCGCTGTTCGTCAGCGCGCCCGCGTCCGGCCAGGGCAAGACGTCGGTCACGGTCGGTCTCGCGCG
GCTGCACCGGCGGCTCGGCCGCCGCGTGCGCGTGTTCAAGACCGGGCCCGATTTTCTCGATCCGATGCTGCTCGAACGCG
CGAGCGGCGCACCCGTGCACGCGCTCGACCTCGGGATGGTCGGCGAGGCCGGTTGCCGCGCGCTGCTCGCCGAAGCCGCG
CGCGACGCGGACCTGATCCTGATCGAAGGCGTGATGGGCTTGTTCGACGGCACGCCGAGCAGCGCCGATCTCGCGGCCGC
GTTCGGCGCGCCGGTCGTCGCGGTGATTTCGGCGAAGGCGATGGCGCAGACGTTCGCGGCGATCGCGTTCGGGCTCGCGC
GGTTTCGGCCGGGGTTGCCGTTTCACGGCGTGCTGGCCAACCGCGTCGGCTCGGCACGGCACGCGGAATTGCTGCAACAG
GCGTTGCCCGACGATCTTCGCTGGCTCGGCCACGTGCCGGCCGATGCGGGTATCGCGCTGCCGGAGCGGCATCTCGGCCT
GCATCAGCCGGCCGATATCGACGATCTCGACGCGCGGCTCGATCGCGCGGCCGATGTGCTCGCGCAGACGGCACTGGCCG
AATTGCCGCCGGCCGTCGCGTTCGCGGAGCCGGACGTTGCCGCGCCGTTGCCGCGCGCGCTTGCCGGCAAGCGGATCGCG
GTGGCGCGCGATGCGGCGTTTTCGTTCATCTATCCGGCGAATCTCGCGTTGCTGGGCGCGCTCGGTGCGCAGTTGCGGTT
CTTTTCGCCGCTTGCCGACGAACCCGTGCCTGACGATTGCGACGCGCTGTTTTTGCCGGGCGGCTATCCGGAACTGCATG
CGGCGACGCTGGCCGCGAATGCCGCGACCGCACGGACGATTCGCGCGCATGCGGCAGCCGGCCGGCCGATCGTCGCGGAA
TGCGGGGGGATGGTGTATCTGTGCGAATCGCTGACCGACGTGGATGGGTCGACGACACCGATGCTCGGCCTGCTGCCGGG
GCATGCGACGATGCAACGCCGGTTCGCGGCGCTCGGCATGCAGCAGCTCGATACTGGCACGGGACCGATGCGCGGTCATA
CGTTTCATTATTCGCGGCTGGATACGCCGCTCGTGCCGGTCGCGACCGCCGCGCGGCCCGATGGCTCGGAGGGCGGCGGC
GAAGCCGTTTATCGCGTGGGCGCGATCGTCGCGACGTACATGCACATGTACTGGCCGTCGAATCCGGATCTGGTCGTGGC
TTTGTTCGGTGGGGAAGTGTTCGAGACATGA

Upstream 100 bases:

>100_bases
CGCTGATCGATGCGGCCGATCTCGTCACCGAAATGCGGCTCGTCAAGCATCCGTACAAGGAGCAAGGCGTGAAGGCGCAG
CGCGGCGTGGAGTTCTGAGC

Downstream 100 bases:

>100_bases
GCGGCCAGCTTGCCGAGCGGCCGACCTTCGACGGTACGACGCCAACGATCTTCAGGATTCGGGGCAGGAATCGCGTGACC
AGACGTGACCGGCCTTAGCC

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPACPALFVSAPASGQGKTSVTVGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVHALDLGMVGEAGCRALLAEAA
RDADLILIEGVMGLFDGTPSSADLAAAFGAPVVAVISAKAMAQTFAAIAFGLARFRPGLPFHGVLANRVGSARHAELLQQ
ALPDDLRWLGHVPADAGIALPERHLGLHQPADIDDLDARLDRAADVLAQTALAELPPAVAFAEPDVAAPLPRALAGKRIA
VARDAAFSFIYPANLALLGALGAQLRFFSPLADEPVPDDCDALFLPGGYPELHAATLAANAATARTIRAHAAAGRPIVAE
CGGMVYLCESLTDVDGSTTPMLGLLPGHATMQRRFAALGMQQLDTGTGPMRGHTFHYSRLDTPLVPVATAARPDGSEGGG
EAVYRVGAIVATYMHMYWPSNPDLVVALFGGEVFET

Sequences:

>Translated_436_residues
MPACPALFVSAPASGQGKTSVTVGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVHALDLGMVGEAGCRALLAEAA
RDADLILIEGVMGLFDGTPSSADLAAAFGAPVVAVISAKAMAQTFAAIAFGLARFRPGLPFHGVLANRVGSARHAELLQQ
ALPDDLRWLGHVPADAGIALPERHLGLHQPADIDDLDARLDRAADVLAQTALAELPPAVAFAEPDVAAPLPRALAGKRIA
VARDAAFSFIYPANLALLGALGAQLRFFSPLADEPVPDDCDALFLPGGYPELHAATLAANAATARTIRAHAAAGRPIVAE
CGGMVYLCESLTDVDGSTTPMLGLLPGHATMQRRFAALGMQQLDTGTGPMRGHTFHYSRLDTPLVPVATAARPDGSEGGG
EAVYRVGAIVATYMHMYWPSNPDLVVALFGGEVFET
>Mature_435_residues
PACPALFVSAPASGQGKTSVTVGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVHALDLGMVGEAGCRALLAEAAR
DADLILIEGVMGLFDGTPSSADLAAAFGAPVVAVISAKAMAQTFAAIAFGLARFRPGLPFHGVLANRVGSARHAELLQQA
LPDDLRWLGHVPADAGIALPERHLGLHQPADIDDLDARLDRAADVLAQTALAELPPAVAFAEPDVAAPLPRALAGKRIAV
ARDAAFSFIYPANLALLGALGAQLRFFSPLADEPVPDDCDALFLPGGYPELHAATLAANAATARTIRAHAAAGRPIVAEC
GGMVYLCESLTDVDGSTTPMLGLLPGHATMQRRFAALGMQQLDTGTGPMRGHTFHYSRLDTPLVPVATAARPDGSEGGGE
AVYRVGAIVATYMHMYWPSNPDLVVALFGGEVFET

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 45469; Mature: 45338

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPACPALFVSAPASGQGKTSVTVGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVH
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCEE
ALDLGMVGEAGCRALLAEAARDADLILIEGVMGLFDGTPSSADLAAAFGAPVVAVISAKA
EEECCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHH
MAQTFAAIAFGLARFRPGLPFHGVLANRVGSARHAELLQQALPDDLRWLGHVPADAGIAL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCC
PERHLGLHQPADIDDLDARLDRAADVLAQTALAELPPAVAFAEPDVAAPLPRALAGKRIA
CHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHCCCEEE
VARDAAFSFIYPANLALLGALGAQLRFFSPLADEPVPDDCDALFLPGGYPELHAATLAAN
EEHHCHHEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHC
AATARTIRAHAAAGRPIVAECGGMVYLCESLTDVDGSTTPMLGLLPGHATMQRRFAALGM
HHHHHHHHHHHCCCCCHHHHCCCEEEEEHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHH
QQLDTGTGPMRGHTFHYSRLDTPLVPVATAARPDGSEGGGEAVYRVGAIVATYMHMYWPS
HHHCCCCCCCCCCEEEHHCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
NPDLVVALFGGEVFET
CCCEEEEEECCHHHCC
>Mature Secondary Structure 
PACPALFVSAPASGQGKTSVTVGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVH
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCEE
ALDLGMVGEAGCRALLAEAARDADLILIEGVMGLFDGTPSSADLAAAFGAPVVAVISAKA
EEECCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHH
MAQTFAAIAFGLARFRPGLPFHGVLANRVGSARHAELLQQALPDDLRWLGHVPADAGIAL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCC
PERHLGLHQPADIDDLDARLDRAADVLAQTALAELPPAVAFAEPDVAAPLPRALAGKRIA
CHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHCCCEEE
VARDAAFSFIYPANLALLGALGAQLRFFSPLADEPVPDDCDALFLPGGYPELHAATLAAN
EEHHCHHEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHC
AATARTIRAHAAAGRPIVAECGGMVYLCESLTDVDGSTTPMLGLLPGHATMQRRFAALGM
HHHHHHHHHHHCCCCCHHHHCCCEEEEEHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHH
QQLDTGTGPMRGHTFHYSRLDTPLVPVATAARPDGSEGGGEAVYRVGAIVATYMHMYWPS
HHHCCCCCCCCCCEEEHHCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
NPDLVVALFGGEVFET
CCCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]