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The map label for this gene is dusA [H]

Identifier: 116689590

GI number: 116689590

Start: 1730938

End: 1732035

Strand: Direct

Name: dusA [H]

Synonym: Bcen2424_1568

Alternate gene names: 116689590

Gene position: 1730938-1732035 (Clockwise)

Preceding gene: 116689589

Following gene: 116689593

Centisome position: 49.68

GC content: 63.21

Gene sequence:

>1098_bases
ATGGCCGGTATTGCGGTAAAATACTCGGTTGTTTCCGTGCCGCCTGGCCTGTACCCGAATTCCATGTCCGTTCCGTCCTC
GCTTCCCCCCCGCCGTGTATCGGTTGCGCCAATGCTCGACTGGACGGACCGCCATTGCCGTTCGTTCCACCGCACGCTGA
CGCGCAATACGTGGCTGTATACGGAGATGATCACGACGGGTGCGCTGCTGTTCGGCGATGCCCAGCGGCATCTCGCGTTC
ACGCCGAGCGAATCGCCAGTCGCGCTGCAACTGGGCGGCAGCGAGCGGGACGACCTCGCGCGCGCGGCGAAACTCGGCGA
GCAGTGGGGCTACGACGAAATCAACCTGAATTGCGGGTGTCCGTCCGAGCGCGTGCAGCGCGGCGCGTTCGGTGCGTGTC
TGATGAACGAGCCGCAGCTCGTCGCCGACTGTGTGCGGGCGATGCGCGATGCGGTGTCGGTGCCGGTGACGGTCAAGCAT
CGGATCGGTGTCGATGCGGTCGAGGATTACGCGTTCGTGCGCGACTTCGTCGGCACGGTGGCCGAGGCCGGTTGCGAAAC
GTTCGTCGTGCATGCCCGCAACGCGATCCTGAAGGGGCTGTCGCCGAAGGAGAACCGCGAGATCCCGCCGCTCAAGTACG
ACTATGCGTATCGGTTGAAGCGCGATTTTCCGTCGCTGGAGATCGTGATCAACGGCGGTATCACGACGCTCGACGAAGTC
GCGCAGCATCTCGAGCATGTCGACGGCGTGATGCTCGGGCGCGAGGCGTATCACAACCCGTACGTGCTGGCGGAGGTCGA
TGCGCGCTTCTACGGGTCGACCGCAGCGGTGCCGATGCGCGAAGAGGCCGAAGCGCAACTGATCGAATACTGCGCGGCTG
AACTGAAGCGCGGAACCTACCTCGGCGCGATCGTGCGGCATGCACTCGGGCTGTATCGCGGCATGCCGGGTGCACGCGGC
TGGCGTCGCGTGCTGTCCGACAACAAGAAGCTCGCACGCGGCGATCTGGCCGTGTTCGACGAGGCTCGCGCGCATCTGAA
CGAAGCCGAAGAAATTTTTGAAAAAAAGGCTTTGCAAGATTCAAAAGTGTTCGTATAA

Upstream 100 bases:

>100_bases
GTTGCTCACCGAGCGGCGCGTGATCGAGCGGGTGTTCGACGAGGCGCGTCGGGCGGTCGATGCGGAGCTGGACGGCCGGT
AGCGCGTGGGTCGGGCGGGT

Downstream 100 bases:

>100_bases
TCTTGTTCTTCGCTGCTGAAACAAAACAGCGAAACGAAGCAAGCAGTATCAGTGGTGGCTGTAGCTCAGTTGGTAGAGTC
CAGGATTGTGATTCCTGTCG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MAGIAVKYSVVSVPPGLYPNSMSVPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRNTWLYTEMITTGALLFGDAQRHLAF
TPSESPVALQLGGSERDDLARAAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVRAMRDAVSVPVTVKH
RIGVDAVEDYAFVRDFVGTVAEAGCETFVVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPSLEIVINGGITTLDEV
AQHLEHVDGVMLGREAYHNPYVLAEVDARFYGSTAAVPMREEAEAQLIEYCAAELKRGTYLGAIVRHALGLYRGMPGARG
WRRVLSDNKKLARGDLAVFDEARAHLNEAEEIFEKKALQDSKVFV

Sequences:

>Translated_365_residues
MAGIAVKYSVVSVPPGLYPNSMSVPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRNTWLYTEMITTGALLFGDAQRHLAF
TPSESPVALQLGGSERDDLARAAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVRAMRDAVSVPVTVKH
RIGVDAVEDYAFVRDFVGTVAEAGCETFVVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPSLEIVINGGITTLDEV
AQHLEHVDGVMLGREAYHNPYVLAEVDARFYGSTAAVPMREEAEAQLIEYCAAELKRGTYLGAIVRHALGLYRGMPGARG
WRRVLSDNKKLARGDLAVFDEARAHLNEAEEIFEKKALQDSKVFV
>Mature_364_residues
AGIAVKYSVVSVPPGLYPNSMSVPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRNTWLYTEMITTGALLFGDAQRHLAFT
PSESPVALQLGGSERDDLARAAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVRAMRDAVSVPVTVKHR
IGVDAVEDYAFVRDFVGTVAEAGCETFVVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPSLEIVINGGITTLDEVA
QHLEHVDGVMLGREAYHNPYVLAEVDARFYGSTAAVPMREEAEAQLIEYCAAELKRGTYLGAIVRHALGLYRGMPGARGW
RRVLSDNKKLARGDLAVFDEARAHLNEAEEIFEKKALQDSKVFV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=239, Percent_Identity=29.2887029288703, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI40807366, Length=257, Percent_Identity=27.2373540856031, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI145693211, Length=312, Percent_Identity=59.6153846153846, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1789660, Length=241, Percent_Identity=24.4813278008299, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788462, Length=188, Percent_Identity=31.9148936170213, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=212, Percent_Identity=29.7169811320755, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=218, Percent_Identity=25.6880733944954, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17507177, Length=179, Percent_Identity=29.608938547486, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=241, Percent_Identity=26.5560165975104, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24580595, Length=251, Percent_Identity=28.6852589641434, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920448, Length=251, Percent_Identity=28.6852589641434, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 40468; Mature: 40336

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGIAVKYSVVSVPPGLYPNSMSVPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRNTWLY
CCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEE
TEMITTGALLFGDAQRHLAFTPSESPVALQLGGSERDDLARAAKLGEQWGYDEINLNCGC
HHHHHHHHHEECCCCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCC
PSERVQRGAFGACLMNEPQLVADCVRAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGTV
CHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHH
AEAGCETFVVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPSLEIVINGGITTLDEV
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHH
AQHLEHVDGVMLGREAYHNPYVLAEVDARFYGSTAAVPMREEAEAQLIEYCAAELKRGTY
HHHHHHHHHHHCCHHHCCCCEEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHH
LGAIVRHALGLYRGMPGARGWRRVLSDNKKLARGDLAVFDEARAHLNEAEEIFEKKALQD
HHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
SKVFV
CCCCC
>Mature Secondary Structure 
AGIAVKYSVVSVPPGLYPNSMSVPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRNTWLY
CCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCEEE
TEMITTGALLFGDAQRHLAFTPSESPVALQLGGSERDDLARAAKLGEQWGYDEINLNCGC
HHHHHHHHHEECCCCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCC
PSERVQRGAFGACLMNEPQLVADCVRAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGTV
CHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHH
AEAGCETFVVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPSLEIVINGGITTLDEV
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHH
AQHLEHVDGVMLGREAYHNPYVLAEVDARFYGSTAAVPMREEAEAQLIEYCAAELKRGTY
HHHHHHHHHHHCCHHHCCCCEEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHH
LGAIVRHALGLYRGMPGARGWRRVLSDNKKLARGDLAVFDEARAHLNEAEEIFEKKALQD
HHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
SKVFV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]