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The map label for this gene is emrB [H]

Identifier: 116689525

GI number: 116689525

Start: 1658330

End: 1659889

Strand: Reverse

Name: emrB [H]

Synonym: Bcen2424_1503

Alternate gene names: 116689525

Gene position: 1659889-1658330 (Counterclockwise)

Preceding gene: 116689526

Following gene: 116689515

Centisome position: 47.64

GC content: 66.6

Gene sequence:

>1560_bases
ATGGCACAGGCTCCTGCCTCCTACCCGCCCTTGCAGGGCGGGCAACTCGTACTCGGGACGATCGCGGTGTCGCTCGCGGT
GTTCATGAACGTGCTCGACACGTCCATCGCGAACGTCGCGATCCCGACCATCTCGGGCGACCTCGGCGTGTCGTCCGACC
AGGGCACGTGGGTCATCACGTCGTTCGCGGTCGCGAACGCCATCTCGGTGCCGCTGACCGGCTGGCTGACCGACCGTTTC
GGGCAGGTGCGCCTGTTCCTCGCGTCGATCATCCTGTTCGTCATCTCGTCGTGGATGTGCGGGCTGTCGCCGAACCTGCC
GTTCCTGCTCGGCTCGCGCGTGCTGCAGGGCGCGGTCGCCGGCCCGATGATTCCGCTGTCGCAAGCGCTCCTGCTGTCGA
GCTATCCGCGCGCGAAAGCACCGATGGCGCTCGCGCTATGGGCGATGACGACGCTGATCGCGCCGGTCGCGGGCCCGATT
CTCGGCGGCTGGATCTCGGACAACTACTCGTGGCCGTGGATCTTCTACGTCAACATCCCGGTCGGCATCGCCGCCGCCGC
CGCGACGTGGATGATCTATCGCAACCGGGAGTCGGCCGTGCGCCGCGCGCCGATCGACGGCGTGGGCCTCGCGCTGCTGG
TCGTCTGGGTCGGCTCGCTGCAGATCATGCTCGACAAGGGCAAGGATCTCGACTGGTTCGCATCGACGACCATCGTCGTG
CTCGCGCTGACCGCCGTCATTGCATTCGCGTTCTTCGTGATCTGGGAGCTGACCGCCGAGCACCCGGTCGTCGACCTGTC
GCTGTTCCGCATCCGGAACTTCACCGGCGGCACGGTCGCGCTGTCGATCGGGTACGGCCTGTACTTCGGCAACCTCGTGC
TGTTGCCGCTGTGGCTGCAGACGCAGATCGGCTACACCGCGACCGACGCCGGTCTCGTGATGGCGCCGGTCGGGCTGTTC
GCCATCCTGCTGTCGCCGCTCACCGGGAAGTATCTGCCGCGCACCGATCCGCGCTTCATCTCGACGGCGTCGTTCCTGAC
GTTTGCGCTGTGCTTCTGGATGCGTTCGCGCTACACGACGGGCGTCGACGAATGGTCGCTGACGCTGCCGACGCTCGTGC
AGGGCATCGCGATGGCGGGCTTCTTCATCCCGCTCGTGTCGATCACGCTGTCCGGCCTGCCCGGCCCCCGCATTCCTGCG
GCGTCCGGCCTGTCGAACTTCGTGCGGATCATGTGCGGCGGCATCGGCACGTCGATCTTCCAGACCGCGTGGGACCACCG
CAACAACTTCCATCACGCGCAACTGGTCGAGCAGGCGAACCCGTACAACCCGACGTTCAACCAGGCCGTCACGCAGATGG
GCAACCTCGGGCTGTCGCAGAGCCAGGCACACGGGCTCATCAACAACATGGCCACGCAACAGGCCGCGCAACTCGGCGTG
AACGATCTGTTCTATATCTCGGCGGTGATCTTCGTGCTGCTGATCGCACTGATCTGGATCACCAAGCCCGAACGCGCGGG
CGGCGGCGATGCCGGTGCGGCGGCGTCGGCAGCGCACTGA

Upstream 100 bases:

>100_bases
GCCGAGAACGCGGGCGGCAACGCATCGGCGCCGGTCGCGGCGAAGCAGAACACCGCCGCGAAAATGATGTAACCGTTCCG
ACTCCGGAAAGACAACCGAC

Downstream 100 bases:

>100_bases
GCGGCCACGTTAAACGCAACACGCCAAATAAAAAGCCCGGTCGAAATCGACCGGGCTTTTTGCATTCTGCCACGCGCACT
TGCGTGCGTTGCTGCGTCAT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MAQAPASYPPLQGGQLVLGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRF
GQVRLFLASIILFVISSWMCGLSPNLPFLLGSRVLQGAVAGPMIPLSQALLLSSYPRAKAPMALALWAMTTLIAPVAGPI
LGGWISDNYSWPWIFYVNIPVGIAAAAATWMIYRNRESAVRRAPIDGVGLALLVVWVGSLQIMLDKGKDLDWFASTTIVV
LALTAVIAFAFFVIWELTAEHPVVDLSLFRIRNFTGGTVALSIGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGLF
AILLSPLTGKYLPRTDPRFISTASFLTFALCFWMRSRYTTGVDEWSLTLPTLVQGIAMAGFFIPLVSITLSGLPGPRIPA
ASGLSNFVRIMCGGIGTSIFQTAWDHRNNFHHAQLVEQANPYNPTFNQAVTQMGNLGLSQSQAHGLINNMATQQAAQLGV
NDLFYISAVIFVLLIALIWITKPERAGGGDAGAAASAAH

Sequences:

>Translated_519_residues
MAQAPASYPPLQGGQLVLGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRF
GQVRLFLASIILFVISSWMCGLSPNLPFLLGSRVLQGAVAGPMIPLSQALLLSSYPRAKAPMALALWAMTTLIAPVAGPI
LGGWISDNYSWPWIFYVNIPVGIAAAAATWMIYRNRESAVRRAPIDGVGLALLVVWVGSLQIMLDKGKDLDWFASTTIVV
LALTAVIAFAFFVIWELTAEHPVVDLSLFRIRNFTGGTVALSIGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGLF
AILLSPLTGKYLPRTDPRFISTASFLTFALCFWMRSRYTTGVDEWSLTLPTLVQGIAMAGFFIPLVSITLSGLPGPRIPA
ASGLSNFVRIMCGGIGTSIFQTAWDHRNNFHHAQLVEQANPYNPTFNQAVTQMGNLGLSQSQAHGLINNMATQQAAQLGV
NDLFYISAVIFVLLIALIWITKPERAGGGDAGAAASAAH
>Mature_518_residues
AQAPASYPPLQGGQLVLGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRFG
QVRLFLASIILFVISSWMCGLSPNLPFLLGSRVLQGAVAGPMIPLSQALLLSSYPRAKAPMALALWAMTTLIAPVAGPIL
GGWISDNYSWPWIFYVNIPVGIAAAAATWMIYRNRESAVRRAPIDGVGLALLVVWVGSLQIMLDKGKDLDWFASTTIVVL
ALTAVIAFAFFVIWELTAEHPVVDLSLFRIRNFTGGTVALSIGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGLFA
ILLSPLTGKYLPRTDPRFISTASFLTFALCFWMRSRYTTGVDEWSLTLPTLVQGIAMAGFFIPLVSITLSGLPGPRIPAA
SGLSNFVRIMCGGIGTSIFQTAWDHRNNFHHAQLVEQANPYNPTFNQAVTQMGNLGLSQSQAHGLINNMATQQAAQLGVN
DLFYISAVIFVLLIALIWITKPERAGGGDAGAAASAAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=495, Percent_Identity=54.7474747474747, Blast_Score=533, Evalue=1e-153,
Organism=Escherichia coli, GI1788710, Length=510, Percent_Identity=48.4313725490196, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1790195, Length=374, Percent_Identity=33.4224598930481, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1788392, Length=402, Percent_Identity=30.3482587064677, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081983, Length=420, Percent_Identity=24.047619047619, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322958, Length=207, Percent_Identity=23.1884057971014, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=28, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=147, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55461; Mature: 55330

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAPASYPPLQGGQLVLGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVIT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEE
SFAVANAISVPLTGWLTDRFGQVRLFLASIILFVISSWMCGLSPNLPFLLGSRVLQGAVA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
GPMIPLSQALLLSSYPRAKAPMALALWAMTTLIAPVAGPILGGWISDNYSWPWIFYVNIP
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
VGIAAAAATWMIYRNRESAVRRAPIDGVGLALLVVWVGSLQIMLDKGKDLDWFASTTIVV
HHHHHHHHHHHEEECHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
LALTAVIAFAFFVIWELTAEHPVVDLSLFRIRNFTGGTVALSIGYGLYFGNLVLLPLWLQ
HHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHH
TQIGYTATDAGLVMAPVGLFAILLSPLTGKYLPRTDPRFISTASFLTFALCFWMRSRYTT
HHCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GVDEWSLTLPTLVQGIAMAGFFIPLVSITLSGLPGPRIPAASGLSNFVRIMCGGIGTSIF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QTAWDHRNNFHHAQLVEQANPYNPTFNQAVTQMGNLGLSQSQAHGLINNMATQQAAQLGV
HHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
NDLFYISAVIFVLLIALIWITKPERAGGGDAGAAASAAH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AQAPASYPPLQGGQLVLGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVIT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEE
SFAVANAISVPLTGWLTDRFGQVRLFLASIILFVISSWMCGLSPNLPFLLGSRVLQGAVA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
GPMIPLSQALLLSSYPRAKAPMALALWAMTTLIAPVAGPILGGWISDNYSWPWIFYVNIP
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
VGIAAAAATWMIYRNRESAVRRAPIDGVGLALLVVWVGSLQIMLDKGKDLDWFASTTIVV
HHHHHHHHHHHEEECHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
LALTAVIAFAFFVIWELTAEHPVVDLSLFRIRNFTGGTVALSIGYGLYFGNLVLLPLWLQ
HHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHH
TQIGYTATDAGLVMAPVGLFAILLSPLTGKYLPRTDPRFISTASFLTFALCFWMRSRYTT
HHCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GVDEWSLTLPTLVQGIAMAGFFIPLVSITLSGLPGPRIPAASGLSNFVRIMCGGIGTSIF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QTAWDHRNNFHHAQLVEQANPYNPTFNQAVTQMGNLGLSQSQAHGLINNMATQQAAQLGV
HHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
NDLFYISAVIFVLLIALIWITKPERAGGGDAGAAASAAH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]