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The map label for this gene is rluB [H]

Identifier: 116689519

GI number: 116689519

Start: 1649625

End: 1651385

Strand: Direct

Name: rluB [H]

Synonym: Bcen2424_1497

Alternate gene names: 116689519

Gene position: 1649625-1651385 (Clockwise)

Preceding gene: 116689518

Following gene: 116689520

Centisome position: 47.35

GC content: 71.72

Gene sequence:

>1761_bases
TTGACAGATATCCACGATATTGAATCGTCCGCGCCTGCCGTGCAGGCAGCGCGTGCAGACGATGCGCCGGAGCAGGATGC
TTCGGCCGCGGGTGGCGACGAGCGTCCCCGCCGCGGTCTGCGGCGCGGCCCGCGTAGCCTGATCGCGCGGCGTCGCGCGG
CGGCCAAGTCGAAGGGCGCCGAAGGCGAGCAGCAGGGTGCCGAGGGCGCGGATGCGCAGCCGGCCGAGGCTGCCGACGCG
CAGCCGGTGCGTGCGCCGCGCAAGGAAGGCGCGGCCAAGGGCGGTCGCAAGCCGGCCGGCAAGCGCGAAGGCGCGGCGGC
GGCCAAGGGCGGCCAGGCTGGCCAGGGCCGTCGCGGTGCAGCGAAGGCTGAAGGCGGCCCGGCGAAGGCGGCGGAAGGCG
ATGCGTCCCAGGACGAACTGTTCGCATACGTGACGTCGCCGGCCTTCGACGCGGACAACTCCGCGGGCGGCAGCGGCGTG
CGTGCGCCGATGCTGCGCCGCGGCCGCGCCCAGCCGGCCAACAAGCGCGTGCTGTCGCCGGACGACGACGCGCCGAAGCT
CCACAAGGTGCTGGCGGAAGCGGGCATGGGGTCGCGCCGCGAAATGGAAGAGCTGATCGTCGCCGGCCGCGTGTCGGTGA
ACGGCGAACCCGCGCACATCGGCCAGCGGATCATGCCGACCGACCAGGTGCGGATCAACGGCAAGCCGGTCAAGCGCAAG
CTGCCGAACAAGCCGCCGCGCGTGCTGCTGTATCACAAGCCGACCGGCGAAATCGTCAGCCACGCGGATCCGGAAGGCCG
TCCGTCGGTGTTCGACCGTCTGCCGCCGATGAAGACGGCGAAATGGCTTGCGGTGGGCCGCCTCGACTTCAATACCGAAG
GTCTGCTGATGCTGACGACTTCCGGCGATCTCGCGAACCGCTTCATGCATCCGCGTTACAGCGTCGAGCGCGAGTACGCG
GTGCGCGTCGTCGGCGAGCTGGCCGAAGGCATGCGGCAAAAGCTGCTGCACGGCGTCGAGCTCGACGACGGTCCGGCGAA
TTTCCTGCGCATCCGCGATGGCGGCGGCGAAGGCACGAACCACTGGTATCACGTCGCGCTGGCCGAAGGCCGCAACCGAG
AAGTGCGCCGGATGTTCGAGGCGGTCGGCCTGATGGTGAGCCGGCTGATCCGTACGCGTCACGGCCCGATTCCGCTGCCG
CGCGGCCTGAAGCGTGGTCGTTGGGAAGAGCTCGACGACGCACAGGTGCGCAAGCTGATGGCGACCGTCGGCCTGAAGGC
GCCGTCCGAGGAGAAGGGCAAGCGCGGCGGCGCTGCCGGTCCGACCGAGCGTCGCCAGCCCGATCCGATGCAGACGTCGA
TGGGCTTCATCAGCCGCGAGCCGGTGCTGACGACGCACGGTCAACTCGACCAGCCGCGTCGCGGCGGTGGTCGACGCGGC
GGGCCGGGCTTGCCGGGCCTGAGCGGCTACGGCAGCTTGCCGGTCGCGCCGTCGGGTTACGGCAACCGCGCGGGCGGCGG
CCGTGACGGCAATCGTACCGGCGGCGGCCGCGACGGCAACCGTGCAGGTGGCGGTCGCGATGTCGACGGCAATCGCGCGT
CGTATGGCGCGGGCCCCAAGCGCGAAGGCGCTGGCGGCAAGCGCGGCGGCGGCAAGGGCGGTGGCAATCGCAATCCGAAC
GGCAATCGTGCCGACGGCGCCGGCAACGGCGGCCCGCGCGGCGGTCAGCGTCCGCAGCGCAGTCGTACGCGCAGCCGCTA
A

Upstream 100 bases:

>100_bases
GCCGGACGACGAAGCAGAACCCGAACAGCGTCGCGCCTGATCGCGGCCAACTTCTTGCCGCGCCCGCGCCGGGCGCGAGA
CCTGACATTTTGAGGTTGTT

Downstream 100 bases:

>100_bases
GCGTCTGTCGATTGACGTACGGCGGCGCAAAGCCGCCGATACGTCGGCGTGGCCGGCATCCGGCCGGCTTTGTCTGCGCG
ACGCATTGCATTGTGAGTTT

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MTDIHDIESSAPAVQAARADDAPEQDASAAGGDERPRRGLRRGPRSLIARRRAAAKSKGAEGEQQGAEGADAQPAEAADA
QPVRAPRKEGAAKGGRKPAGKREGAAAAKGGQAGQGRRGAAKAEGGPAKAAEGDASQDELFAYVTSPAFDADNSAGGSGV
RAPMLRRGRAQPANKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRK
LPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYA
VRVVGELAEGMRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLP
RGLKRGRWEELDDAQVRKLMATVGLKAPSEEKGKRGGAAGPTERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGGRRG
GPGLPGLSGYGSLPVAPSGYGNRAGGGRDGNRTGGGRDGNRAGGGRDVDGNRASYGAGPKREGAGGKRGGGKGGGNRNPN
GNRADGAGNGGPRGGQRPQRSRTRSR

Sequences:

>Translated_586_residues
MTDIHDIESSAPAVQAARADDAPEQDASAAGGDERPRRGLRRGPRSLIARRRAAAKSKGAEGEQQGAEGADAQPAEAADA
QPVRAPRKEGAAKGGRKPAGKREGAAAAKGGQAGQGRRGAAKAEGGPAKAAEGDASQDELFAYVTSPAFDADNSAGGSGV
RAPMLRRGRAQPANKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRK
LPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYA
VRVVGELAEGMRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLP
RGLKRGRWEELDDAQVRKLMATVGLKAPSEEKGKRGGAAGPTERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGGRRG
GPGLPGLSGYGSLPVAPSGYGNRAGGGRDGNRTGGGRDGNRAGGGRDVDGNRASYGAGPKREGAGGKRGGGKGGGNRNPN
GNRADGAGNGGPRGGQRPQRSRTRSR
>Mature_585_residues
TDIHDIESSAPAVQAARADDAPEQDASAAGGDERPRRGLRRGPRSLIARRRAAAKSKGAEGEQQGAEGADAQPAEAADAQ
PVRAPRKEGAAKGGRKPAGKREGAAAAKGGQAGQGRRGAAKAEGGPAKAAEGDASQDELFAYVTSPAFDADNSAGGSGVR
APMLRRGRAQPANKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRKL
PNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYAV
RVVGELAEGMRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPR
GLKRGRWEELDDAQVRKLMATVGLKAPSEEKGKRGGAAGPTERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGGRRGG
PGLPGLSGYGSLPVAPSGYGNRAGGGRDGNRTGGGRDGNRAGGGRDVDGNRASYGAGPKREGAGGKRGGGKGGGNRNPNG
NRADGAGNGGPRGGQRPQRSRTRSR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=298, Percent_Identity=51.6778523489933, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1790453, Length=253, Percent_Identity=30.0395256916996, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081838, Length=192, Percent_Identity=34.8958333333333, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788510, Length=218, Percent_Identity=29.8165137614679, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 61828; Mature: 61697

Theoretical pI: Translated: 11.29; Mature: 11.29

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIHDIESSAPAVQAARADDAPEQDASAAGGDERPRRGLRRGPRSLIARRRAAAKSKGA
CCCCHHHHCCCCHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCC
EGEQQGAEGADAQPAEAADAQPVRAPRKEGAAKGGRKPAGKREGAAAAKGGQAGQGRRGA
CCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCC
AKAEGGPAKAAEGDASQDELFAYVTSPAFDADNSAGGSGVRAPMLRRGRAQPANKRVLSP
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
DDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRK
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCCHHHHCCCCCCCCEEEECCCCHHHH
LPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTT
CCCCCCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCHHEEEEECEEECCCCEEEEEC
SGDLANRFMHPRYSVEREYAVRVVGELAEGMRQKLLHGVELDDGPANFLRIRDGGGEGTN
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCC
HWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKRGRWEELDDAQVRKLM
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ATVGLKAPSEEKGKRGGAAGPTERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGGRRG
HHHCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCC
GPGLPGLSGYGSLPVAPSGYGNRAGGGRDGNRTGGGRDGNRAGGGRDVDGNRASYGAGPK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
REGAGGKRGGGKGGGNRNPNGNRADGAGNGGPRGGQRPQRSRTRSR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
TDIHDIESSAPAVQAARADDAPEQDASAAGGDERPRRGLRRGPRSLIARRRAAAKSKGA
CCCHHHHCCCCHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCC
EGEQQGAEGADAQPAEAADAQPVRAPRKEGAAKGGRKPAGKREGAAAAKGGQAGQGRRGA
CCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCC
AKAEGGPAKAAEGDASQDELFAYVTSPAFDADNSAGGSGVRAPMLRRGRAQPANKRVLSP
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
DDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRK
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCCHHHHCCCCCCCCEEEECCCCHHHH
LPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTT
CCCCCCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCHHEEEEECEEECCCCEEEEEC
SGDLANRFMHPRYSVEREYAVRVVGELAEGMRQKLLHGVELDDGPANFLRIRDGGGEGTN
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCC
HWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKRGRWEELDDAQVRKLM
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ATVGLKAPSEEKGKRGGAAGPTERRQPDPMQTSMGFISREPVLTTHGQLDQPRRGGGRRG
HHHCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCC
GPGLPGLSGYGSLPVAPSGYGNRAGGGRDGNRTGGGRDGNRAGGGRDVDGNRASYGAGPK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
REGAGGKRGGGKGGGNRNPNGNRADGAGNGGPRGGQRPQRSRTRSR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]