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The map label for this gene is phoB [H]

Identifier: 116689458

GI number: 116689458

Start: 1584414

End: 1585118

Strand: Direct

Name: phoB [H]

Synonym: Bcen2424_1436

Alternate gene names: 116689458

Gene position: 1584414-1585118 (Clockwise)

Preceding gene: 116689457

Following gene: 116689459

Centisome position: 45.48

GC content: 63.69

Gene sequence:

>705_bases
ATGAGAATTGCTGTACTGGATGACGATCCGGCCCAGACGGATTTCGTCAGTCAAACGCTGACGGCCGTTGGCCATACGTG
CTATGCGTTCAAGGAAGGCAAGGCCCTGAAGAAGCGCCTGCAGCGCGAGACGTTCGATCTGCTGGTGCTCGACTGGAACG
TGCCCGACATGTCCGGCGAGGAAGTGCTCAAGTGGGTGCGCGCCAACCAGACCGAGCACAGCCTGCCGATCATCTTCATG
ACGAGCCGCGACGACGAGGCGGGGATCACGCAGATCCTCAACGCCGGCGCCGACGATTACGTGGTCAAGCCGGTGTCCGG
CCCGATCCTGCGCGCGCGCATCGGTTCGCTGCTGCGCCGCGCGTATCCGGTCAACGCCGAGGCGACCGTCCGCGAATTCG
ATCAGTTCCGTTTCGACGTGAACCTGAAGCAGGCGTACGTCGGCGACAAGGCCGTGAGCCTCACGCAAAAGGAATTCGAG
CTCGCGCTGTTGCTGTTCCAGCATCTGGACCGGCCGCTGTCGCGCGCGCACATTCTCGACCTCGTGTGGAAGCAGGCGAC
CGACATTCCGTCGCGCACGATGGATACGCACATTTCGATGCTGCGCACGAAGCTCGGCCTGCGGCCCGAGAACGGCTACC
GTCTCGCGCCGATCTACGGGTACGGCTACCGGCTCGAACGGGTCGGCCAGGGGGAACCGGAGTGA

Upstream 100 bases:

>100_bases
CGACGGCTCGCTGGCCTGTATCCGCCGCCGCGCGCAGGTTGCGCGGCCGGGGCAGGCGCGGGAGCCGAATGGACGAATCG
ACTTCGATTTGCGGGGTGCT

Downstream 100 bases:

>100_bases
CGACGCGAATCACGCAGCGCACGGTGAACCTGCGCACGGCGGCGCTGCGCGCGGCCTGCGCGGTCGCGTTGGCGTTCGCC
GCGCAGCAGGCCGCGGCGCA

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MRIAVLDDDPAQTDFVSQTLTAVGHTCYAFKEGKALKKRLQRETFDLLVLDWNVPDMSGEEVLKWVRANQTEHSLPIIFM
TSRDDEAGITQILNAGADDYVVKPVSGPILRARIGSLLRRAYPVNAEATVREFDQFRFDVNLKQAYVGDKAVSLTQKEFE
LALLLFQHLDRPLSRAHILDLVWKQATDIPSRTMDTHISMLRTKLGLRPENGYRLAPIYGYGYRLERVGQGEPE

Sequences:

>Translated_234_residues
MRIAVLDDDPAQTDFVSQTLTAVGHTCYAFKEGKALKKRLQRETFDLLVLDWNVPDMSGEEVLKWVRANQTEHSLPIIFM
TSRDDEAGITQILNAGADDYVVKPVSGPILRARIGSLLRRAYPVNAEATVREFDQFRFDVNLKQAYVGDKAVSLTQKEFE
LALLLFQHLDRPLSRAHILDLVWKQATDIPSRTMDTHISMLRTKLGLRPENGYRLAPIYGYGYRLERVGQGEPE
>Mature_234_residues
MRIAVLDDDPAQTDFVSQTLTAVGHTCYAFKEGKALKKRLQRETFDLLVLDWNVPDMSGEEVLKWVRANQTEHSLPIIFM
TSRDDEAGITQILNAGADDYVVKPVSGPILRARIGSLLRRAYPVNAEATVREFDQFRFDVNLKQAYVGDKAVSLTQKEFE
LALLLFQHLDRPLSRAHILDLVWKQATDIPSRTMDTHISMLRTKLGLRPENGYRLAPIYGYGYRLERVGQGEPE

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790860, Length=228, Percent_Identity=32.0175438596491, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=28.8888888888889, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=28.9473684210526, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789402, Length=227, Percent_Identity=29.5154185022026, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1790552, Length=225, Percent_Identity=27.1111111111111, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=29.1479820627803, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786784, Length=229, Percent_Identity=27.0742358078603, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788394, Length=232, Percent_Identity=29.3103448275862, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786911, Length=226, Percent_Identity=26.5486725663717, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787229, Length=211, Percent_Identity=28.9099526066351, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787375, Length=227, Percent_Identity=25.1101321585903, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=30.1310043668122, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790863, Length=230, Percent_Identity=24.3478260869565, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=27.2727272727273, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26605; Mature: 26605

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAVLDDDPAQTDFVSQTLTAVGHTCYAFKEGKALKKRLQRETFDLLVLDWNVPDMSGE
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEEECCCCCCCHH
EVLKWVRANQTEHSLPIIFMTSRDDEAGITQILNAGADDYVVKPVSGPILRARIGSLLRR
HHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
AYPVNAEATVREFDQFRFDVNLKQAYVGDKAVSLTQKEFELALLLFQHLDRPLSRAHILD
HCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LVWKQATDIPSRTMDTHISMLRTKLGLRPENGYRLAPIYGYGYRLERVGQGEPE
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCC
>Mature Secondary Structure
MRIAVLDDDPAQTDFVSQTLTAVGHTCYAFKEGKALKKRLQRETFDLLVLDWNVPDMSGE
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEEECCCCCCCHH
EVLKWVRANQTEHSLPIIFMTSRDDEAGITQILNAGADDYVVKPVSGPILRARIGSLLRR
HHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
AYPVNAEATVREFDQFRFDVNLKQAYVGDKAVSLTQKEFELALLLFQHLDRPLSRAHILD
HCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LVWKQATDIPSRTMDTHISMLRTKLGLRPENGYRLAPIYGYGYRLERVGQGEPE
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]