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The map label for this gene is rnhA [H]

Identifier: 116689307

GI number: 116689307

Start: 1413229

End: 1413672

Strand: Reverse

Name: rnhA [H]

Synonym: Bcen2424_1285

Alternate gene names: 116689307

Gene position: 1413672-1413229 (Counterclockwise)

Preceding gene: 116689308

Following gene: 116689306

Centisome position: 40.58

GC content: 64.86

Gene sequence:

>444_bases
ATGACAACCGACACCATCGACATCTATACCGACGGCGCCTGCAAGGGCAACCCCGGCCCCGGCGGCTGGGGCGCCCTACT
GCGCTACGGCAGTCAAGAAAAGGAACTGTTCGGCGGCGAGCCGAACACGACCAACAACCGCATGGAGCTGATGGGCGTGA
TCGCCGCGCTCGAAGCGCTGAAGCGGCCGTGCCGGGTGATCGTGCATACCGACTCGCAATATGTGCAGAAAGGCATCAGC
GAGTGGATTCACGGATGGAAGAAGAAAGGCTGGGTCACCGCGGCGAAGACGCCCGTGAAGAATGCCGACCTGTGGAAGCG
GCTCGACGCGCTCGTCGCTCAGCACGAAATCGAGTGGCGCTGGGTCAAGGGCCACGCGGGCCATCCCGAAAACGAGCGCG
CGGACGCGCTCGCGAACCGCGGCGTCGAATCGCTGGCCGCCTGA

Upstream 100 bases:

>100_bases
TGCGCGGCATGCGCCTCGTCGGCCCGATCAAGATGAAGAAGCCCGTGCTCGCACCGGGCCTGACGCCGGCGGCCTCCCCG
ACCACTCACCAAGAACGTTC

Downstream 100 bases:

>100_bases
ACCCCGGCCGCCCTCTCTTGTTTTCATTGCTTTTCCCGATATGCGCCAGATCATTCTCGATACCGAAACCACCGGCCTGA
ACGCCCGCACGGGCGACCGT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MTTDTIDIYTDGACKGNPGPGGWGALLRYGSQEKELFGGEPNTTNNRMELMGVIAALEALKRPCRVIVHTDSQYVQKGIS
EWIHGWKKKGWVTAAKTPVKNADLWKRLDALVAQHEIEWRWVKGHAGHPENERADALANRGVESLAA

Sequences:

>Translated_147_residues
MTTDTIDIYTDGACKGNPGPGGWGALLRYGSQEKELFGGEPNTTNNRMELMGVIAALEALKRPCRVIVHTDSQYVQKGIS
EWIHGWKKKGWVTAAKTPVKNADLWKRLDALVAQHEIEWRWVKGHAGHPENERADALANRGVESLAA
>Mature_146_residues
TTDTIDIYTDGACKGNPGPGGWGALLRYGSQEKELFGGEPNTTNNRMELMGVIAALEALKRPCRVIVHTDSQYVQKGISE
WIHGWKKKGWVTAAKTPVKNADLWKRLDALVAQHEIEWRWVKGHAGHPENERADALANRGVESLAA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=151, Percent_Identity=35.0993377483444, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1786408, Length=133, Percent_Identity=66.9172932330827, Blast_Score=197, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI115532542, Length=146, Percent_Identity=39.7260273972603, Blast_Score=96, Evalue=5e-21,
Organism=Drosophila melanogaster, GI17137476, Length=146, Percent_Identity=33.5616438356164, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45552509, Length=146, Percent_Identity=33.5616438356164, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16261; Mature: 16129

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDTIDIYTDGACKGNPGPGGWGALLRYGSQEKELFGGEPNTTNNRMELMGVIAALEAL
CCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KRPCRVIVHTDSQYVQKGISEWIHGWKKKGWVTAAKTPVKNADLWKRLDALVAQHEIEWR
HCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEE
WVKGHAGHPENERADALANRGVESLAA
EEECCCCCCCCHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
TTDTIDIYTDGACKGNPGPGGWGALLRYGSQEKELFGGEPNTTNNRMELMGVIAALEAL
CCCEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KRPCRVIVHTDSQYVQKGISEWIHGWKKKGWVTAAKTPVKNADLWKRLDALVAQHEIEWR
HCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEE
WVKGHAGHPENERADALANRGVESLAA
EEECCCCCCCCHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]