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The map label for this gene is proP [H]

Identifier: 116689303

GI number: 116689303

Start: 1410073

End: 1411563

Strand: Reverse

Name: proP [H]

Synonym: Bcen2424_1281

Alternate gene names: 116689303

Gene position: 1411563-1410073 (Counterclockwise)

Preceding gene: 116689304

Following gene: 116689300

Centisome position: 40.52

GC content: 68.08

Gene sequence:

>1491_bases
ATGACCTTGACCGCAACACACGTCAGCCCGACCGCTGTCTCTTCCCCCGCTTCGTCGTCCGGCGAAGCCCCGCTCGCCGC
GGACGACATCACCGTCGTCGACCAGAGCCTGCTCAAGCGCGCCGTCAGCGCAATGGCCATCGGCAATGCGATGGAATGGT
TCGACTTCGGCGTCTACAGCTATATCGCCGTCACGCTCGGCAAGGTGTTCTTTCCGTCCAGCAGCCCGTCCGCGCAGTTG
CTGGCCACCTTCGGCACGTTCGCGGCCGCGTTCCTCGTGCGCCCGCTCGGCGGCATGGTGTTCGGCCCGCTCGGCGACCG
CATCGGCCGCCAGCGCGTGCTCGCCGCCACGATGATCATGATGGCCGTCGGCACCTTCGCGATCGGCCTGATCCCGAGCT
ATGCGTCGATCGGCATCATGGCCCCGATCCTGCTGCTCGTCGCCCGCCTCGTGCAGGGCTTCTCGACCGGCGGCGAGTAC
GGCGGCGCCGCCACTTTCATCGCCGAGTTCTCGACCGACAAGCGCCGCGGCTTCATGGGCAGCTTCCTCGAATTCGGCAC
GCTGATCGGCTATGTGCTGGGCGCGGGCGTCGTCGCACTGCTCACCGCGTCGCTGTCGCAGGAAGCGCTGCTGTCGTGGG
GCTGGCGCGTGCCGTTCCTGATCGCCGGGCCGCTCGGCCTGATCGGTCTGTACATCCGGATGAAGCTCGAGGAAACGCCG
GCGTTCAAGCGCCAGGCCGAAGAGCGCGAAGCGCAGGACAAGGCCGTGCCGAAGGCGCGCTTTCGCGAGACGCTGCTGCG
CAACTGGCGTGCGCTGCTGCTGTGCGTCGGCCTCGTGCTGATCTTCAACGTGACCGACTACATGGTGCTGTCTTACCTGC
CGAGCTTCATGTCGTCGACGCTGCACTTCGACGAATCGCACAGCCTCGTGCTGGTGCTGATCGTGATGGTGCTGATGATG
CCGATGACGCTGTACGCCGGCCGCCTGTCGGACAAGGTCGGCCGCAAGCCGGTGATGCTCGCCGGCTGCGTCGGCCTGCT
GGTGCTGTCGATCCCGTCGCTGATGCTGATCCACACGGGCACCACGGCGTCGGTGTTCGGCGGCCTGCTGATCCTCGGCG
TGCTGCTGTCGTGCTTCACCGGCGTGATGCCGTCGGCGCTGCCCGCGCTGTTCCCGACCGAGATCCGCTACGGCGCGCTC
GCGATCGGCTTCAACGTGTCGGTGTCGCTGTTCGGCGGCACGACGCCGCTGGTGACCGCGTGGCTCGTCGACGTGACGCA
CAACCTGATGATGCCCGCGTACTACATGATGGGCGCCGCGCTGATCGGCATCGTGTCGGTCGTCGCGCTCGCCGAAACCG
CGCGCCAGCCGCTGAAGGGCTCGCCGCCGGCCGTCGCGACGCGCCGCGAAGCGCACCAGCTGGCGCGCCAGTTGCGCGAG
GACGACGACGAATCGGAGGTCTACGGCGTCGCGACGCCCGCCCGCGCGTAA

Upstream 100 bases:

>100_bases
AAGTGCTTAATGGTCTGCCCCGACGCGCAGCACACGACACCGCGCTCGCCGGCAAACCGTGTCGGACGGCCTGCCCGTTC
CGGCGCTGTCAAAGGAGTGC

Downstream 100 bases:

>100_bases
GCGTCGCGCAATGAAAAAGCCCCGGCCGAAGCCGGGGCCCGATCGAACCCGATCTGCCCGACCGGCTTGCGCCGGCCGGG
CTTTTTTCATTCAGTCGGCA

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MTLTATHVSPTAVSSPASSSGEAPLAADDITVVDQSLLKRAVSAMAIGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQL
LATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAATMIMMAVGTFAIGLIPSYASIGIMAPILLLVARLVQGFSTGGEY
GGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVLGAGVVALLTASLSQEALLSWGWRVPFLIAGPLGLIGLYIRMKLEETP
AFKRQAEEREAQDKAVPKARFRETLLRNWRALLLCVGLVLIFNVTDYMVLSYLPSFMSSTLHFDESHSLVLVLIVMVLMM
PMTLYAGRLSDKVGRKPVMLAGCVGLLVLSIPSLMLIHTGTTASVFGGLLILGVLLSCFTGVMPSALPALFPTEIRYGAL
AIGFNVSVSLFGGTTPLVTAWLVDVTHNLMMPAYYMMGAALIGIVSVVALAETARQPLKGSPPAVATRREAHQLARQLRE
DDDESEVYGVATPARA

Sequences:

>Translated_496_residues
MTLTATHVSPTAVSSPASSSGEAPLAADDITVVDQSLLKRAVSAMAIGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQL
LATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAATMIMMAVGTFAIGLIPSYASIGIMAPILLLVARLVQGFSTGGEY
GGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVLGAGVVALLTASLSQEALLSWGWRVPFLIAGPLGLIGLYIRMKLEETP
AFKRQAEEREAQDKAVPKARFRETLLRNWRALLLCVGLVLIFNVTDYMVLSYLPSFMSSTLHFDESHSLVLVLIVMVLMM
PMTLYAGRLSDKVGRKPVMLAGCVGLLVLSIPSLMLIHTGTTASVFGGLLILGVLLSCFTGVMPSALPALFPTEIRYGAL
AIGFNVSVSLFGGTTPLVTAWLVDVTHNLMMPAYYMMGAALIGIVSVVALAETARQPLKGSPPAVATRREAHQLARQLRE
DDDESEVYGVATPARA
>Mature_495_residues
TLTATHVSPTAVSSPASSSGEAPLAADDITVVDQSLLKRAVSAMAIGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQLL
ATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAATMIMMAVGTFAIGLIPSYASIGIMAPILLLVARLVQGFSTGGEYG
GAATFIAEFSTDKRRGFMGSFLEFGTLIGYVLGAGVVALLTASLSQEALLSWGWRVPFLIAGPLGLIGLYIRMKLEETPA
FKRQAEEREAQDKAVPKARFRETLLRNWRALLLCVGLVLIFNVTDYMVLSYLPSFMSSTLHFDESHSLVLVLIVMVLMMP
MTLYAGRLSDKVGRKPVMLAGCVGLLVLSIPSLMLIHTGTTASVFGGLLILGVLLSCFTGVMPSALPALFPTEIRYGALA
IGFNVSVSLFGGTTPLVTAWLVDVTHNLMMPAYYMMGAALIGIVSVVALAETARQPLKGSPPAVATRREAHQLARQLRED
DDESEVYGVATPARA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=474, Percent_Identity=50.42194092827, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1788292, Length=433, Percent_Identity=31.4087759815243, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1788942, Length=405, Percent_Identity=34.5679012345679, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789941, Length=426, Percent_Identity=33.3333333333333, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI87082231, Length=430, Percent_Identity=23.4883720930233, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082404, Length=365, Percent_Identity=23.2876712328767, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 52838; Mature: 52707

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTATHVSPTAVSSPASSSGEAPLAADDITVVDQSLLKRAVSAMAIGNAMEWFDFGVYS
CCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YIAVTLGKVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAATMIM
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHH
MAVGTFAIGLIPSYASIGIMAPILLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHH
SFLEFGTLIGYVLGAGVVALLTASLSQEALLSWGWRVPFLIAGPLGLIGLYIRMKLEETP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEECHHHHHHHHHHEEECCCC
AFKRQAEEREAQDKAVPKARFRETLLRNWRALLLCVGLVLIFNVTDYMVLSYLPSFMSST
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHFDESHSLVLVLIVMVLMMPMTLYAGRLSDKVGRKPVMLAGCVGLLVLSIPSLMLIHTG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEECC
TTASVFGGLLILGVLLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLVTA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECEEEEEEECCCHHHHHH
WLVDVTHNLMMPAYYMMGAALIGIVSVVALAETARQPLKGSPPAVATRREAHQLARQLRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
DDDESEVYGVATPARA
CCCCCCEEECCCCCCC
>Mature Secondary Structure 
TLTATHVSPTAVSSPASSSGEAPLAADDITVVDQSLLKRAVSAMAIGNAMEWFDFGVYS
CEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YIAVTLGKVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAATMIM
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHH
MAVGTFAIGLIPSYASIGIMAPILLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHH
SFLEFGTLIGYVLGAGVVALLTASLSQEALLSWGWRVPFLIAGPLGLIGLYIRMKLEETP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEECHHHHHHHHHHEEECCCC
AFKRQAEEREAQDKAVPKARFRETLLRNWRALLLCVGLVLIFNVTDYMVLSYLPSFMSST
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHFDESHSLVLVLIVMVLMMPMTLYAGRLSDKVGRKPVMLAGCVGLLVLSIPSLMLIHTG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEECC
TTASVFGGLLILGVLLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLVTA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECEEEEEEECCCHHHHHH
WLVDVTHNLMMPAYYMMGAALIGIVSVVALAETARQPLKGSPPAVATRREAHQLARQLRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
DDDESEVYGVATPARA
CCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]