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The map label for this gene is ynfL [H]

Identifier: 116689278

GI number: 116689278

Start: 1389235

End: 1390218

Strand: Reverse

Name: ynfL [H]

Synonym: Bcen2424_1256

Alternate gene names: 116689278

Gene position: 1390218-1389235 (Counterclockwise)

Preceding gene: 116689280

Following gene: 116689277

Centisome position: 39.9

GC content: 70.22

Gene sequence:

>984_bases
ATGCCTTCCAATACCATCGAATGGCGTTTGCTATGCTTTACAGGCATATCCATCCATCGAACCGGACACCGCATGCGCCA
GATCGAATTGCGTCACCTTCGCTACTTCGTCGCCGTCGCCGAGGCCGGCAGCGTGATGGCCGGCGCCCGCGCGGCCGGCA
TCGTGCAGCCCGCGCTGTCGCGGCAGATCCGCGAGCTGGAGGACGCGATCGGCACGCCGCTGCTGATCCGTCGCGCGACG
GGCGTCACGCTCACCGCGGCCGGCACGAGCTTTCTGCACGATGCCGCCGGCCTGCTCGCGATGCTGCAGGACAGCCGTGA
GCGCGCGCTGCGCAGCGCGGCCGGCCAGCTCGGCGAACTGCGGCTTGGCGCGCTGCCGAACTGCCTGCCGTTGCCGGTCG
TCGCGAACGTCCTCAAAACATTTCGCGATGCGTGCCCGGACGTGAAACTGTCGATCGCGCCGATGCTGTCCGCCGAGCAG
GCCAGCGCGCTGATGCGCGGCCAGCTCGACGCCGGAATCATGGCGTGGCGTCGCGAGGAAGCGCCGCACCTGACGGGCGT
GCGGCTGCTGAGCGACCGCTTCGTGCTCGCGATGCCCGCACCGTCGGGCGGCCGCTTCGTCGCGCCGCGCACGCTGGCCG
ACGTCGCGAACGAGCCGTTCGTCTGGTTCGATGCGCAACGTTCCGCCGCGCACCACCGGTTCCTGATGACGCAGTGCCAG
CAGGCCGGTTTCACGCCGCGCATCGCGCAGGTCGGCAGCGATATCCCGACGCTGATCGGCCTCGTCGCGGTGGGGATGGG
CTGCGCGTTCGTGCCGGAAAGCGCATCGCCGACCTGCCCGAGCACGGTTCGGCTCGTCGCGCTCGACGAACTCGCGAGCC
GCTTCGACATCGAGTTCGTGTTCGACGGCGCGGCGACGACGCCGTCGCCCGTCGTCGCGCGGTTTCTCGCGGCGGTCCGC
GATGCGGCCGGCGAACCGGGCTGA

Upstream 100 bases:

>100_bases
TGAAGCGGCATGGGGCGGGACGCAGTGGGGACGGAGGAAACACACGCGACTCCGGAGGAAGCGAGCGCGGCGATGACGGT
CATCGTACGGCAGGCCGGCC

Downstream 100 bases:

>100_bases
ACGCACGCTGGACGCATCGGCGCGCGGTCGGGCGATGCGACTTGCCGCGCAGACCGACGTGCGGAATTCACTTCCGCGAT
CGCGTCGCTTTCGATGTCTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPSNTIEWRLLCFTGISIHRTGHRMRQIELRHLRYFVAVAEAGSVMAGARAAGIVQPALSRQIRELEDAIGTPLLIRRAT
GVTLTAAGTSFLHDAAGLLAMLQDSRERALRSAAGQLGELRLGALPNCLPLPVVANVLKTFRDACPDVKLSIAPMLSAEQ
ASALMRGQLDAGIMAWRREEAPHLTGVRLLSDRFVLAMPAPSGGRFVAPRTLADVANEPFVWFDAQRSAAHHRFLMTQCQ
QAGFTPRIAQVGSDIPTLIGLVAVGMGCAFVPESASPTCPSTVRLVALDELASRFDIEFVFDGAATTPSPVVARFLAAVR
DAAGEPG

Sequences:

>Translated_327_residues
MPSNTIEWRLLCFTGISIHRTGHRMRQIELRHLRYFVAVAEAGSVMAGARAAGIVQPALSRQIRELEDAIGTPLLIRRAT
GVTLTAAGTSFLHDAAGLLAMLQDSRERALRSAAGQLGELRLGALPNCLPLPVVANVLKTFRDACPDVKLSIAPMLSAEQ
ASALMRGQLDAGIMAWRREEAPHLTGVRLLSDRFVLAMPAPSGGRFVAPRTLADVANEPFVWFDAQRSAAHHRFLMTQCQ
QAGFTPRIAQVGSDIPTLIGLVAVGMGCAFVPESASPTCPSTVRLVALDELASRFDIEFVFDGAATTPSPVVARFLAAVR
DAAGEPG
>Mature_326_residues
PSNTIEWRLLCFTGISIHRTGHRMRQIELRHLRYFVAVAEAGSVMAGARAAGIVQPALSRQIRELEDAIGTPLLIRRATG
VTLTAAGTSFLHDAAGLLAMLQDSRERALRSAAGQLGELRLGALPNCLPLPVVANVLKTFRDACPDVKLSIAPMLSAEQA
SALMRGQLDAGIMAWRREEAPHLTGVRLLSDRFVLAMPAPSGGRFVAPRTLADVANEPFVWFDAQRSAAHHRFLMTQCQQ
AGFTPRIAQVGSDIPTLIGLVAVGMGCAFVPESASPTCPSTVRLVALDELASRFDIEFVFDGAATTPSPVVARFLAAVRD
AAGEPG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=265, Percent_Identity=36.6037735849057, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1788887, Length=249, Percent_Identity=32.9317269076305, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI2367136, Length=302, Percent_Identity=29.4701986754967, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787601, Length=250, Percent_Identity=28, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI157672245, Length=236, Percent_Identity=28.8135593220339, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789506, Length=236, Percent_Identity=25.8474576271186, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=27.0588235294118, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081904, Length=148, Percent_Identity=32.4324324324324, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=26.984126984127, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790208, Length=265, Percent_Identity=26.4150943396226, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34967; Mature: 34836

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSNTIEWRLLCFTGISIHRTGHRMRQIELRHLRYFVAVAEAGSVMAGARAAGIVQPALS
CCCCCEEEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RQIRELEDAIGTPLLIRRATGVTLTAAGTSFLHDAAGLLAMLQDSRERALRSAAGQLGEL
HHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RLGALPNCLPLPVVANVLKTFRDACPDVKLSIAPMLSAEQASALMRGQLDAGIMAWRREE
HHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHCHHHHHHHHHCCCCHHHHHHHHCC
APHLTGVRLLSDRFVLAMPAPSGGRFVAPRTLADVANEPFVWFDAQRSAAHHRFLMTQCQ
CCCHHHHHEECCCEEEEECCCCCCCEECCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
QAGFTPRIAQVGSDIPTLIGLVAVGMGCAFVPESASPTCPSTVRLVALDELASRFDIEFV
HCCCCCHHHHHCCCHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEHHHHHHHCCEEEE
FDGAATTPSPVVARFLAAVRDAAGEPG
ECCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PSNTIEWRLLCFTGISIHRTGHRMRQIELRHLRYFVAVAEAGSVMAGARAAGIVQPALS
CCCCEEEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RQIRELEDAIGTPLLIRRATGVTLTAAGTSFLHDAAGLLAMLQDSRERALRSAAGQLGEL
HHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RLGALPNCLPLPVVANVLKTFRDACPDVKLSIAPMLSAEQASALMRGQLDAGIMAWRREE
HHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHCHHHHHHHHHCCCCHHHHHHHHCC
APHLTGVRLLSDRFVLAMPAPSGGRFVAPRTLADVANEPFVWFDAQRSAAHHRFLMTQCQ
CCCHHHHHEECCCEEEEECCCCCCCEECCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
QAGFTPRIAQVGSDIPTLIGLVAVGMGCAFVPESASPTCPSTVRLVALDELASRFDIEFV
HCCCCCHHHHHCCCHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEHHHHHHHCCEEEE
FDGAATTPSPVVARFLAAVRDAAGEPG
ECCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]