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The map label for this gene is fliM [H]

Identifier: 116624443

GI number: 116624443

Start: 6726330

End: 6727211

Strand: Reverse

Name: fliM [H]

Synonym: Acid_5365

Alternate gene names: 116624443

Gene position: 6727211-6726330 (Counterclockwise)

Preceding gene: 116624453

Following gene: 116624439

Centisome position: 67.5

GC content: 60.54

Gene sequence:

>882_bases
GTGCGCGCCATCCACCTGCTGCACGACACGTTCGTGCGCAATCTGGTATCCAGCCTGTCGGCGTACCTGCGCTCCTATCT
CACGGTGAACCTGGTCAGCGTGGAGCAGCTGTCCTACGCCGAATTCCTCGACGGCCTGCCGTCACCCACCTGTATTGTTT
CGCTGGGGCTTAGCCCGTACGATGGCAATGGTGTGCTCGAACTCAATCCGAGCCTGGTTTTCCCGATTCTGGAGATGCTG
CTGGGCGGCACAGGCAAGAGTTCGGCCACGATCCAGCGCGATATCACGGAAATCGAACAGCGCCTGCTTGACGGTTTATT
TCGCATCATCCTTCAGGACCTGCGCGAGGCCTGGAAGGCCGTCACTAATGTGGATTTCACGATCGAGAGCATGGAGACGG
AGCCTCAGTTGCTGCACCTGCTAGCCCCCAACGAAGCGGTGGTGTCGATCGGTATCGAAGTCCGCATCGGCGAGACCGTC
GGAATGATGAACATCGCGATGCCCTCGATCGTGATCAAGATGATGCGGCAGAAGTTCGATCAGCAGTGGTCGGTTCGTAA
GACGCACGCCAGCGAGGCCGAACAATTCCGCGTATTACGTGTGCTGCGACAGGCGACTCTTACCGTAGAGGCGCTGATGG
AAGGTCCGACGCTCTCCGTCGGCGACCTGTTGAGTTTACGGGAAGGCAACCTGTTGACCTTCGACTTTCCGGTGGAACGC
CCGATCGAGCTGGTGGTAAACGGAGCGTGCAAGTTCACCGGACAGGTGGTGAGCACAGGGCGGAAACGCGCCTGCCAGAT
CGGCACGGTGCGGCCGCTTCCCTATCAGGGCAGGATCAGCGACGACGCGGGCGCAGGCTCGGGCTCGGGGCCGCCGGAAT
AG

Upstream 100 bases:

>100_bases
GGAAATCGACGCAGTTTTCCAGAACATCCAGGAGCGGAAGCGCGAAGCGCCGGCGGTCAAGTTCGACTTCCGTCGTCCTG
ACCGCATCCCGAAATCGCAG

Downstream 100 bases:

>100_bases
AAGCGGCGCAGGGCGTCGATGAAGGCGCGGCCGCGGCGGCGGCCGTTGTTTTGATGGAGTTCGAAATGTTGAAGAAAGAC
GAGCATTCCGAGAACGGCGG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MRAIHLLHDTFVRNLVSSLSAYLRSYLTVNLVSVEQLSYAEFLDGLPSPTCIVSLGLSPYDGNGVLELNPSLVFPILEML
LGGTGKSSATIQRDITEIEQRLLDGLFRIILQDLREAWKAVTNVDFTIESMETEPQLLHLLAPNEAVVSIGIEVRIGETV
GMMNIAMPSIVIKMMRQKFDQQWSVRKTHASEAEQFRVLRVLRQATLTVEALMEGPTLSVGDLLSLREGNLLTFDFPVER
PIELVVNGACKFTGQVVSTGRKRACQIGTVRPLPYQGRISDDAGAGSGSGPPE

Sequences:

>Translated_293_residues
MRAIHLLHDTFVRNLVSSLSAYLRSYLTVNLVSVEQLSYAEFLDGLPSPTCIVSLGLSPYDGNGVLELNPSLVFPILEML
LGGTGKSSATIQRDITEIEQRLLDGLFRIILQDLREAWKAVTNVDFTIESMETEPQLLHLLAPNEAVVSIGIEVRIGETV
GMMNIAMPSIVIKMMRQKFDQQWSVRKTHASEAEQFRVLRVLRQATLTVEALMEGPTLSVGDLLSLREGNLLTFDFPVER
PIELVVNGACKFTGQVVSTGRKRACQIGTVRPLPYQGRISDDAGAGSGSGPPE
>Mature_293_residues
MRAIHLLHDTFVRNLVSSLSAYLRSYLTVNLVSVEQLSYAEFLDGLPSPTCIVSLGLSPYDGNGVLELNPSLVFPILEML
LGGTGKSSATIQRDITEIEQRLLDGLFRIILQDLREAWKAVTNVDFTIESMETEPQLLHLLAPNEAVVSIGIEVRIGETV
GMMNIAMPSIVIKMMRQKFDQQWSVRKTHASEAEQFRVLRVLRQATLTVEALMEGPTLSVGDLLSLREGNLLTFDFPVER
PIELVVNGACKFTGQVVSTGRKRACQIGTVRPLPYQGRISDDAGAGSGSGPPE

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=271, Percent_Identity=24.7232472324723, Blast_Score=98, Evalue=6e-22,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 32195; Mature: 32195

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAIHLLHDTFVRNLVSSLSAYLRSYLTVNLVSVEQLSYAEFLDGLPSPTCIVSLGLSPY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCCCCEEEEECCCCC
DGNGVLELNPSLVFPILEMLLGGTGKSSATIQRDITEIEQRLLDGLFRIILQDLREAWKA
CCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTNVDFTIESMETEPQLLHLLAPNEAVVSIGIEVRIGETVGMMNIAMPSIVIKMMRQKFD
HHCCCEEEECCCCCCCEEEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
QQWSVRKTHASEAEQFRVLRVLRQATLTVEALMEGPTLSVGDLLSLREGNLLTFDFPVER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCC
PIELVVNGACKFTGQVVSTGRKRACQIGTVRPLPYQGRISDDAGAGSGSGPPE
CHHHHCCCCHHHHHHHHHCCCHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRAIHLLHDTFVRNLVSSLSAYLRSYLTVNLVSVEQLSYAEFLDGLPSPTCIVSLGLSPY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCCCCEEEEECCCCC
DGNGVLELNPSLVFPILEMLLGGTGKSSATIQRDITEIEQRLLDGLFRIILQDLREAWKA
CCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTNVDFTIESMETEPQLLHLLAPNEAVVSIGIEVRIGETVGMMNIAMPSIVIKMMRQKFD
HHCCCEEEECCCCCCCEEEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
QQWSVRKTHASEAEQFRVLRVLRQATLTVEALMEGPTLSVGDLLSLREGNLLTFDFPVER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCC
PIELVVNGACKFTGQVVSTGRKRACQIGTVRPLPYQGRISDDAGAGSGSGPPE
CHHHHCCCCHHHHHHHHHCCCHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]