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The map label for this gene is afr [H]

Identifier: 116622291

GI number: 116622291

Start: 4034280

End: 4035254

Strand: Direct

Name: afr [H]

Synonym: Acid_3185

Alternate gene names: 116622291

Gene position: 4034280-4035254 (Clockwise)

Preceding gene: 116622289

Following gene: 116622295

Centisome position: 40.48

GC content: 63.9

Gene sequence:

>975_bases
ATGCTCTCTTGGCTGGTGGTTGGCATCGGGGATATCACCCGGAAACGAGTGTTGCCGGCAATCCTGGCGGAGCGGCGGAG
CCGGCTGGCGGGCATCGTAACGCGCGATTCCCTGAAAGCCGCGACCTACGGAGTGCCGTCATGGACGGACCTGGATAGCG
CCCTCGATGCCTGCGAGTGCGAGGCGGTCTACATCGCGACGCCGGTGTTTCTGCACTCTCAGCAAACCATCGCCGCGCTG
CGAGCGGGCCGCCACGTGATCTGCGAAAAGCCGATGGCGCTCGATTACCCGCAAGCAGAGGAAATGAACAGAACTGCGCA
GGCGAGCGTCCGAAAACTTGCAATCGCCTACTACCGGCGCCTTTATCCAAAGGTGGAGCGAGCGCGCCACCTGATCGAAA
GAGGCGTAATCGGTCGGCCCCTGATCGCGGAAGCGACGGCGCATGATTGGTTCAACCCGGTGGGCACCTCGCGGGCCTGG
CTGGCCGACCCCGCGCAGGCCGGTCACGGACCGCTGCGCGACGTTGCCTCGCACCGTATCGACCTGATGAATTACCTGTT
CGGAAAGCCGGTGCGGGCGTCGGGTTTTCTCTCTACGCTGGTGCAGCCGCTGCCGGTAGAAGACAGCGCGACGGTCATGA
TCGATTACCAGGGAGGGGTCCGGGCCGTAGTGGACGCTCGGTGGAACTCGCGCGTCGCGCGCGACGAATTCCGGGTCCGC
GGCACGGATGGCGAAATCGATCTCTCGCCGCTCAACGGCCCGGAATTACGGTATCCGGGCGGGCAGGAGTCCATTCCGGC
GCCGGCGAACCTGCACTACCCTTTCGTGGAAGATTTTGTCACGTCGGTGCTGCGCGGCATCGATCCGGTCTCTAATGGCG
CGAGCGCGCTGGCGGCGGAATGGGTGATGGACGAGGCCGCCGCTACTTCACGATTGTCCAATGCAGAAGAAAGTACGATA
GCCAGGCGATCGTAG

Upstream 100 bases:

>100_bases
TTGATCAAAGGAAACTGTTGCATAGTTTACCTCTGAACGCGGTGTGTGGTAGCACGTTCTCTGCCAAAACTGGTACTAGT
ATGGATGATACGGTAAGAAG

Downstream 100 bases:

>100_bases
CCGCCGCCGGGATGGTCAGGATCCACGCCCACACGATATTGGTGGCGATTCCCCAACGTACGGCCTTCATGCGCTGGATG
CTGCCCACGCCCGCGATGGC

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MLSWLVVGIGDITRKRVLPAILAERRSRLAGIVTRDSLKAATYGVPSWTDLDSALDACECEAVYIATPVFLHSQQTIAAL
RAGRHVICEKPMALDYPQAEEMNRTAQASVRKLAIAYYRRLYPKVERARHLIERGVIGRPLIAEATAHDWFNPVGTSRAW
LADPAQAGHGPLRDVASHRIDLMNYLFGKPVRASGFLSTLVQPLPVEDSATVMIDYQGGVRAVVDARWNSRVARDEFRVR
GTDGEIDLSPLNGPELRYPGGQESIPAPANLHYPFVEDFVTSVLRGIDPVSNGASALAAEWVMDEAAATSRLSNAEESTI
ARRS

Sequences:

>Translated_324_residues
MLSWLVVGIGDITRKRVLPAILAERRSRLAGIVTRDSLKAATYGVPSWTDLDSALDACECEAVYIATPVFLHSQQTIAAL
RAGRHVICEKPMALDYPQAEEMNRTAQASVRKLAIAYYRRLYPKVERARHLIERGVIGRPLIAEATAHDWFNPVGTSRAW
LADPAQAGHGPLRDVASHRIDLMNYLFGKPVRASGFLSTLVQPLPVEDSATVMIDYQGGVRAVVDARWNSRVARDEFRVR
GTDGEIDLSPLNGPELRYPGGQESIPAPANLHYPFVEDFVTSVLRGIDPVSNGASALAAEWVMDEAAATSRLSNAEESTI
ARRS
>Mature_324_residues
MLSWLVVGIGDITRKRVLPAILAERRSRLAGIVTRDSLKAATYGVPSWTDLDSALDACECEAVYIATPVFLHSQQTIAAL
RAGRHVICEKPMALDYPQAEEMNRTAQASVRKLAIAYYRRLYPKVERARHLIERGVIGRPLIAEATAHDWFNPVGTSRAW
LADPAQAGHGPLRDVASHRIDLMNYLFGKPVRASGFLSTLVQPLPVEDSATVMIDYQGGVRAVVDARWNSRVARDEFRVR
GTDGEIDLSPLNGPELRYPGGQESIPAPANLHYPFVEDFVTSVLRGIDPVSNGASALAAEWVMDEAAATSRLSNAEESTI
ARRS

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=279, Percent_Identity=26.1648745519713, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787574, Length=199, Percent_Identity=30.1507537688442, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082405, Length=207, Percent_Identity=26.5700483091787, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789848, Length=254, Percent_Identity=25.9842519685039, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 35554; Mature: 35554

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSWLVVGIGDITRKRVLPAILAERRSRLAGIVTRDSLKAATYGVPSWTDLDSALDACEC
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHCC
EAVYIATPVFLHSQQTIAALRAGRHVICEKPMALDYPQAEEMNRTAQASVRKLAIAYYRR
CEEEEECCCEECCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LYPKVERARHLIERGVIGRPLIAEATAHDWFNPVGTSRAWLADPAQAGHGPLRDVASHRI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHH
DLMNYLFGKPVRASGFLSTLVQPLPVEDSATVMIDYQGGVRAVVDARWNSRVARDEFRVR
HHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCCCHHCCHHEEE
GTDGEIDLSPLNGPELRYPGGQESIPAPANLHYPFVEDFVTSVLRGIDPVSNGASALAAE
CCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHH
WVMDEAAATSRLSNAEESTIARRS
HHHHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MLSWLVVGIGDITRKRVLPAILAERRSRLAGIVTRDSLKAATYGVPSWTDLDSALDACEC
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHCC
EAVYIATPVFLHSQQTIAALRAGRHVICEKPMALDYPQAEEMNRTAQASVRKLAIAYYRR
CEEEEECCCEECCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LYPKVERARHLIERGVIGRPLIAEATAHDWFNPVGTSRAWLADPAQAGHGPLRDVASHRI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHH
DLMNYLFGKPVRASGFLSTLVQPLPVEDSATVMIDYQGGVRAVVDARWNSRVARDEFRVR
HHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCCCCCHHCCHHEEE
GTDGEIDLSPLNGPELRYPGGQESIPAPANLHYPFVEDFVTSVLRGIDPVSNGASALAAE
CCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHH
WVMDEAAATSRLSNAEESTIARRS
HHHHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA