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The map label for this gene is rhmD [H]

Identifier: 116622211

GI number: 116622211

Start: 3929371

End: 3930570

Strand: Reverse

Name: rhmD [H]

Synonym: Acid_3103

Alternate gene names: 116622211

Gene position: 3930570-3929371 (Counterclockwise)

Preceding gene: 116622212

Following gene: 116622210

Centisome position: 39.44

GC content: 60.17

Gene sequence:

>1200_bases
ATGGCATTCGCGGATTCACCCCAGGACTCCAGGAAGGCCCCCAGAAGACTTCCCAATCCGGCGGATTTGGACCGCGCCGC
GGCTCTCCCGGTGTTGAAGGTGGACGGCTTGAAATCGCCCGTCATCATTGACTCCATGAAGCTCTTGAAGAGCGGCAATG
ATTACCTGGTGCATGTGCGCTCTAAAGACGGCGCTGAAGGCGTCTCCATCACCAATCCTCCTCGTGCGCAATATCTGGAC
CACGTCTTCCAGCAGCTGGTGGCGCCGTTCTTCATCGGCAAGGACGCGCGCGACCTGGATTCCCTGCTGCGCGGTCTCTA
CCGCTGGAACAGCAATTACAAGATGTACGGGCTCGGGTTCTGGAGCGCTCAGGCCTGGGCCGAGTTCGCCATCCTCGACA
TGCTGGGCAGAGTCGCCAATAAACCGATCGGTGCCTTGCTGGGCGGCGTTGTGCGTAAGGACATGCCGTACTACGTGGCC
AGCGGACGCCGCGATACCACGCCCGAACAGGAGATCGAATACCTGCAAAAGCTGCTGTCGGATTCCGGCGCCAAGGCGCT
GAAGTTCCGCGTGGGCGGCCGCATGAGCGAGAACGAAGATGCCATGCCGCAGCGGACCGAGAAGCTCATCCCGCTGGTCC
GAAAAACCTTCGGCGACGCTATGGATATCCACGCAGATGCCAACAGCTCCTACGATCCCAAGGAAGCCATCCGCGTGGGA
CACCTCCTCGAAGAAATCAAAGCCGTGCATTACGAAGAACCGTGCGAGTTCGACCACTTCGATGAAATGAAGACAGTGGC
CGACGCCCTGACGGTGCCGGTCGCCTGCGGCGAACAGGAATTCAGCCAGTGGCGATTCCGCTGGATGATCGCCAACCACG
GTGCCGACATCGTGCAGCCCGACCTGTTCTACTACGGCGGCTTGATCCGCTCCATCCGCGTGGCGCGCATGGCCGGAGTG
GCCGGTTTGCCGATCGCACTGCACGTTTCGGGCGGCTTCGGCTTTGTGTACGTACTCCATTTCGCCTCCTGCACGCCAAA
CCTCGCGCGCTATCAGGAATACAAGCTGGGCATGGAAAAATACGGGAAGTGGCTGGACCCGGGGATTCAGGTCAAGGATG
GCAAAATGAGCATCCCGACCGGTCCAGGCGTCGGAATCAAAGACCTCGCCGGCCTCCTGAAAGACGCCAAAGAAGTTTAG

Upstream 100 bases:

>100_bases
GCAGGTGCAGTTCGGTCTGAAGTTCGTCTACTAACGGAACAACCATATGAAAGAAATCGGTCGCAGAAGTTTGCTCGCTG
GTGTCGTAGGCGCGGGCGGC

Downstream 100 bases:

>100_bases
GTCTCCGACGGCGAGGTTTTTGCGTGATTCGCCAGGACGCTCGCGGCTCCGATCTGATCCTTGTTTTCGTTAACCGTGCT
GCTACCGTTACGCCCGGATT

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAFADSPQDSRKAPRRLPNPADLDRAAALPVLKVDGLKSPVIIDSMKLLKSGNDYLVHVRSKDGAEGVSITNPPRAQYLD
HVFQQLVAPFFIGKDARDLDSLLRGLYRWNSNYKMYGLGFWSAQAWAEFAILDMLGRVANKPIGALLGGVVRKDMPYYVA
SGRRDTTPEQEIEYLQKLLSDSGAKALKFRVGGRMSENEDAMPQRTEKLIPLVRKTFGDAMDIHADANSSYDPKEAIRVG
HLLEEIKAVHYEEPCEFDHFDEMKTVADALTVPVACGEQEFSQWRFRWMIANHGADIVQPDLFYYGGLIRSIRVARMAGV
AGLPIALHVSGGFGFVYVLHFASCTPNLARYQEYKLGMEKYGKWLDPGIQVKDGKMSIPTGPGVGIKDLAGLLKDAKEV

Sequences:

>Translated_399_residues
MAFADSPQDSRKAPRRLPNPADLDRAAALPVLKVDGLKSPVIIDSMKLLKSGNDYLVHVRSKDGAEGVSITNPPRAQYLD
HVFQQLVAPFFIGKDARDLDSLLRGLYRWNSNYKMYGLGFWSAQAWAEFAILDMLGRVANKPIGALLGGVVRKDMPYYVA
SGRRDTTPEQEIEYLQKLLSDSGAKALKFRVGGRMSENEDAMPQRTEKLIPLVRKTFGDAMDIHADANSSYDPKEAIRVG
HLLEEIKAVHYEEPCEFDHFDEMKTVADALTVPVACGEQEFSQWRFRWMIANHGADIVQPDLFYYGGLIRSIRVARMAGV
AGLPIALHVSGGFGFVYVLHFASCTPNLARYQEYKLGMEKYGKWLDPGIQVKDGKMSIPTGPGVGIKDLAGLLKDAKEV
>Mature_398_residues
AFADSPQDSRKAPRRLPNPADLDRAAALPVLKVDGLKSPVIIDSMKLLKSGNDYLVHVRSKDGAEGVSITNPPRAQYLDH
VFQQLVAPFFIGKDARDLDSLLRGLYRWNSNYKMYGLGFWSAQAWAEFAILDMLGRVANKPIGALLGGVVRKDMPYYVAS
GRRDTTPEQEIEYLQKLLSDSGAKALKFRVGGRMSENEDAMPQRTEKLIPLVRKTFGDAMDIHADANSSYDPKEAIRVGH
LLEEIKAVHYEEPCEFDHFDEMKTVADALTVPVACGEQEFSQWRFRWMIANHGADIVQPDLFYYGGLIRSIRVARMAGVA
GLPIALHVSGGFGFVYVLHFASCTPNLARYQEYKLGMEKYGKWLDPGIQVKDGKMSIPTGPGVGIKDLAGLLKDAKEV

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=290, Percent_Identity=25.5172413793103, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787864, Length=367, Percent_Identity=23.7057220708447, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 44353; Mature: 44222

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFADSPQDSRKAPRRLPNPADLDRAAALPVLKVDGLKSPVIIDSMKLLKSGNDYLVHVR
CCCCCCCCHHHHCHHCCCCCCCCHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEE
SKDGAEGVSITNPPRAQYLDHVFQQLVAPFFIGKDARDLDSLLRGLYRWNSNYKMYGLGF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
WSAQAWAEFAILDMLGRVANKPIGALLGGVVRKDMPYYVASGRRDTTPEQEIEYLQKLLS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHC
DSGAKALKFRVGGRMSENEDAMPQRTEKLIPLVRKTFGDAMDIHADANSSYDPKEAIRVG
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
HLLEEIKAVHYEEPCEFDHFDEMKTVADALTVPVACGEQEFSQWRFRWMIANHGADIVQP
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEEECCCCCCCCC
DLFYYGGLIRSIRVARMAGVAGLPIALHVSGGFGFVYVLHFASCTPNLARYQEYKLGMEK
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCHHH
YGKWLDPGIQVKDGKMSIPTGPGVGIKDLAGLLKDAKEV
CCCCCCCCCEEECCEEECCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFADSPQDSRKAPRRLPNPADLDRAAALPVLKVDGLKSPVIIDSMKLLKSGNDYLVHVR
CCCCCCCHHHHCHHCCCCCCCCHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEE
SKDGAEGVSITNPPRAQYLDHVFQQLVAPFFIGKDARDLDSLLRGLYRWNSNYKMYGLGF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
WSAQAWAEFAILDMLGRVANKPIGALLGGVVRKDMPYYVASGRRDTTPEQEIEYLQKLLS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHC
DSGAKALKFRVGGRMSENEDAMPQRTEKLIPLVRKTFGDAMDIHADANSSYDPKEAIRVG
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHH
HLLEEIKAVHYEEPCEFDHFDEMKTVADALTVPVACGEQEFSQWRFRWMIANHGADIVQP
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEEECCCCCCCCC
DLFYYGGLIRSIRVARMAGVAGLPIALHVSGGFGFVYVLHFASCTPNLARYQEYKLGMEK
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCHHH
YGKWLDPGIQVKDGKMSIPTGPGVGIKDLAGLLKDAKEV
CCCCCCCCCEEECCEEECCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA