Click here to switch to the map view.

The map label for this gene is ilvB [H]

Identifier: 116622207

GI number: 116622207

Start: 3923562

End: 3925328

Strand: Direct

Name: ilvB [H]

Synonym: Acid_3099

Alternate gene names: 116622207

Gene position: 3923562-3925328 (Clockwise)

Preceding gene: 116622206

Following gene: 116622208

Centisome position: 39.37

GC content: 64.52

Gene sequence:

>1767_bases
ATGAAGACCGCGGATTTGATCGTTCGCACGCATATCAGTGAGGGTGAAAAGCGGATTGCGGCTTACCAACTGGTGGATTA
CCTGGAGAGGCTCGGTGTCGAAGTAATTTTCGGCCTCTGCGGCCATACCGTCATCGCCTTCCTGGATGCGCTGCGCGGCA
GCCGGATCAAGTTCATCTCTACCCGACACGAACAGGTGGCGGCGCACGCGGCCGATGGTTACGCGCGCGCCTCGGGCAAG
CCCGGCGTACTGATGACTCACCTGGGTCCGGGACTTACCAATGCCGCAACCGGCGTGGCCAACGCGGCGCTCGATTCAAT
CCCGATGGTGGTCATCGCGGGCGATGTTCCGTCGCACTACTTTGGGCGGCATCCACACCAGGAAGTCAACCTGCACATGG
ATGCGGACCAGTACGAGATCTACCGGCCGTTCTGCAAACGCATCTACCGCGTGGACCGCGTGGCGGACCTGCCGCGCATT
GTGGAGCGGGCATTCCACCTCTCGCAGGCCGGCCGTCCCGGACCGGTGCTGGTGGATGTGCCGATGGACATCTTCTCCGC
CGATCTTCCGGCGGGCGCGTTCTCGCAGATGCCGGCACCAATCGTGCGGCCCACGATCGATGCTTCCACGGCGGAGCGGA
TCGCCCAGGCTCTGGCGGAGGCCGAGCGTCCGGTGTTGTACGTGGGCGGCGGAGTGCTCGGGGGGAATGCGACTAACGAA
CTGCTGGCGCTTGCCGAGGCGCTGGAGGTTCCGGTGGCGCATACCCTGATGGGCAAGGGGTGCATGCGCGAGGACCATCC
GCTGCTGCTCGGCATGACCGGCTTCTGGGGTACGCCGATCGCCAACGAGACTTGCCGCACGGCGGACCTGATCGTCGCCG
TCGGGACCCGGCTGGCGGAGGCGAACTCGAGTTCGTGGGATCCGCGGTTCACGTTCGATATTCCACCGACACGCCTGATT
CACATCGATATCGACGCGGCCGAGATCGGGCGGAATTTCGCCACGGAACTCGGCGTGGTCGCGGACTCGCGGATGGCGCT
GGGCGCAATCGCGCATGCCGCACGCGGAAAGTACCGTTCGGAACGCGGCGACCTGCGCGAGCGAATCGCCGCCGGACGCG
CCGCATTCGCGTCGACTTGGGCGGACCAATGGAGTTCGGATCAGTACCCGATGCGGCCCGAGCGGATTCTCAGTGAATTG
AAGAAGGCTATTCCCGAGGACGGCTTCATCGTGACTGATGTTGGTTGGAACAAGAACGGAGTGGGCCAGCAGTATCCGAT
CACGGTTCCCGGTACGTTCGTCACGCCGAGCGGACTGGCGACGATGGGCTTCGGACCCTCGGCGGTGCTGGGCGTCAAAA
TGGCGCAGCCGCAGCGCGCGGCGGTCGCGCTGATCGGGGACGGCGGGTTCGGCAGCAATCCTTCGGTGGTGGCGACGGCG
ATGGAGGCCGAGCTCGCGGTAGTGTGGCTGGTGATGGACAACTCCGCGTACGGTACGATTGCGGGGCTGGAAAAGGCGCA
CTACGGCACCAGCTTCGGGTGCCTGTTTGAACGCCAGGGCCGGCCGTACAGCGTGGATTACGCCGCGCTCGCGCGCTCTT
ATGGCGCCGGGGGAATTCGGATTAGCGCTGCGGACGAACTTGGCCCGGCGCTGCGCGAGGCGCTCGCGTCCGGCATGCCG
ACGGTGATTCAGGCTGAGATGGAGAATGCTCCCACGCCTACACCGGGGCACTGGAATATTAACGATATTTATCGTAGAGG
AGACTAA

Upstream 100 bases:

>100_bases
CAAGAACTTCGAGGAAGTGCGCAAAGTGCCGGGCCTGGATGCCGCCAAGATCGGCGACAAGCGCGGCTGGATGTCATTTG
AGTGAAATGGGGGTATTGAG

Downstream 100 bases:

>100_bases
CGTGGCGGCGTGGCCAGTGCGCGGAAAGGAGGGGCGCGCGGTCGGCGGGGTGCCGAGCATGAACGGAGAAGCCGCCTGAA
AGGCGGCTGCAGGCAGGATT

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MKTADLIVRTHISEGEKRIAAYQLVDYLERLGVEVIFGLCGHTVIAFLDALRGSRIKFISTRHEQVAAHAADGYARASGK
PGVLMTHLGPGLTNAATGVANAALDSIPMVVIAGDVPSHYFGRHPHQEVNLHMDADQYEIYRPFCKRIYRVDRVADLPRI
VERAFHLSQAGRPGPVLVDVPMDIFSADLPAGAFSQMPAPIVRPTIDASTAERIAQALAEAERPVLYVGGGVLGGNATNE
LLALAEALEVPVAHTLMGKGCMREDHPLLLGMTGFWGTPIANETCRTADLIVAVGTRLAEANSSSWDPRFTFDIPPTRLI
HIDIDAAEIGRNFATELGVVADSRMALGAIAHAARGKYRSERGDLRERIAAGRAAFASTWADQWSSDQYPMRPERILSEL
KKAIPEDGFIVTDVGWNKNGVGQQYPITVPGTFVTPSGLATMGFGPSAVLGVKMAQPQRAAVALIGDGGFGSNPSVVATA
MEAELAVVWLVMDNSAYGTIAGLEKAHYGTSFGCLFERQGRPYSVDYAALARSYGAGGIRISAADELGPALREALASGMP
TVIQAEMENAPTPTPGHWNINDIYRRGD

Sequences:

>Translated_588_residues
MKTADLIVRTHISEGEKRIAAYQLVDYLERLGVEVIFGLCGHTVIAFLDALRGSRIKFISTRHEQVAAHAADGYARASGK
PGVLMTHLGPGLTNAATGVANAALDSIPMVVIAGDVPSHYFGRHPHQEVNLHMDADQYEIYRPFCKRIYRVDRVADLPRI
VERAFHLSQAGRPGPVLVDVPMDIFSADLPAGAFSQMPAPIVRPTIDASTAERIAQALAEAERPVLYVGGGVLGGNATNE
LLALAEALEVPVAHTLMGKGCMREDHPLLLGMTGFWGTPIANETCRTADLIVAVGTRLAEANSSSWDPRFTFDIPPTRLI
HIDIDAAEIGRNFATELGVVADSRMALGAIAHAARGKYRSERGDLRERIAAGRAAFASTWADQWSSDQYPMRPERILSEL
KKAIPEDGFIVTDVGWNKNGVGQQYPITVPGTFVTPSGLATMGFGPSAVLGVKMAQPQRAAVALIGDGGFGSNPSVVATA
MEAELAVVWLVMDNSAYGTIAGLEKAHYGTSFGCLFERQGRPYSVDYAALARSYGAGGIRISAADELGPALREALASGMP
TVIQAEMENAPTPTPGHWNINDIYRRGD
>Mature_588_residues
MKTADLIVRTHISEGEKRIAAYQLVDYLERLGVEVIFGLCGHTVIAFLDALRGSRIKFISTRHEQVAAHAADGYARASGK
PGVLMTHLGPGLTNAATGVANAALDSIPMVVIAGDVPSHYFGRHPHQEVNLHMDADQYEIYRPFCKRIYRVDRVADLPRI
VERAFHLSQAGRPGPVLVDVPMDIFSADLPAGAFSQMPAPIVRPTIDASTAERIAQALAEAERPVLYVGGGVLGGNATNE
LLALAEALEVPVAHTLMGKGCMREDHPLLLGMTGFWGTPIANETCRTADLIVAVGTRLAEANSSSWDPRFTFDIPPTRLI
HIDIDAAEIGRNFATELGVVADSRMALGAIAHAARGKYRSERGDLRERIAAGRAAFASTWADQWSSDQYPMRPERILSEL
KKAIPEDGFIVTDVGWNKNGVGQQYPITVPGTFVTPSGLATMGFGPSAVLGVKMAQPQRAAVALIGDGGFGSNPSVVATA
MEAELAVVWLVMDNSAYGTIAGLEKAHYGTSFGCLFERQGRPYSVDYAALARSYGAGGIRISAADELGPALREALASGMP
TVIQAEMENAPTPTPGHWNINDIYRRGD

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=26.1565836298932, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI21361361, Length=570, Percent_Identity=24.9122807017544, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI87081685, Length=536, Percent_Identity=33.5820895522388, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1790104, Length=569, Percent_Identity=32.1616871704745, Blast_Score=255, Evalue=7e-69,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=29.4117647058824, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1787096, Length=556, Percent_Identity=26.978417266187, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788716, Length=568, Percent_Identity=23.943661971831, Blast_Score=132, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17542570, Length=598, Percent_Identity=26.5886287625418, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17531299, Length=579, Percent_Identity=26.2521588946459, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17531301, Length=579, Percent_Identity=26.2521588946459, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=563, Percent_Identity=29.4849023090586, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6320816, Length=556, Percent_Identity=23.5611510791367, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323073, Length=535, Percent_Identity=25.4205607476636, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6323163, Length=490, Percent_Identity=22.8571428571429, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922626, Length=583, Percent_Identity=25.557461406518, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62986; Mature: 62986

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTADLIVRTHISEGEKRIAAYQLVDYLERLGVEVIFGLCGHTVIAFLDALRGSRIKFIS
CCCHHEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
TRHEQVAAHAADGYARASGKPGVLMTHLGPGLTNAATGVANAALDSIPMVVIAGDVPSHY
CHHHHHHHHHCCCHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHH
FGRHPHQEVNLHMDADQYEIYRPFCKRIYRVDRVADLPRIVERAFHLSQAGRPGPVLVDV
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PMDIFSADLPAGAFSQMPAPIVRPTIDASTAERIAQALAEAERPVLYVGGGVLGGNATNE
CHHHHHCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEECCCCHHH
LLALAEALEVPVAHTLMGKGCMREDHPLLLGMTGFWGTPIANETCRTADLIVAVGTRLAE
HHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
ANSSSWDPRFTFDIPPTRLIHIDIDAAEIGRNFATELGVVADSRMALGAIAHAARGKYRS
CCCCCCCCEEEEECCCCEEEEEECCHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCH
ERGDLRERIAAGRAAFASTWADQWSSDQYPMRPERILSELKKAIPEDGFIVTDVGWNKNG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
VGQQYPITVPGTFVTPSGLATMGFGPSAVLGVKMAQPQRAAVALIGDGGFGSNPSVVATA
CCCCCCEECCCCEECCCCCEECCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCEEEEE
MEAELAVVWLVMDNSAYGTIAGLEKAHYGTSFGCLFERQGRPYSVDYAALARSYGAGGIR
EECCEEEEEEEECCCCCEEHHCCHHHCCCCCEEEEEECCCCCEECHHHHHHHHCCCCCEE
ISAADELGPALREALASGMPTVIQAEMENAPTPTPGHWNINDIYRRGD
EECHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MKTADLIVRTHISEGEKRIAAYQLVDYLERLGVEVIFGLCGHTVIAFLDALRGSRIKFIS
CCCHHEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
TRHEQVAAHAADGYARASGKPGVLMTHLGPGLTNAATGVANAALDSIPMVVIAGDVPSHY
CHHHHHHHHHCCCHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHH
FGRHPHQEVNLHMDADQYEIYRPFCKRIYRVDRVADLPRIVERAFHLSQAGRPGPVLVDV
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PMDIFSADLPAGAFSQMPAPIVRPTIDASTAERIAQALAEAERPVLYVGGGVLGGNATNE
CHHHHHCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEECCCCHHH
LLALAEALEVPVAHTLMGKGCMREDHPLLLGMTGFWGTPIANETCRTADLIVAVGTRLAE
HHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
ANSSSWDPRFTFDIPPTRLIHIDIDAAEIGRNFATELGVVADSRMALGAIAHAARGKYRS
CCCCCCCCEEEEECCCCEEEEEECCHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCH
ERGDLRERIAAGRAAFASTWADQWSSDQYPMRPERILSELKKAIPEDGFIVTDVGWNKNG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
VGQQYPITVPGTFVTPSGLATMGFGPSAVLGVKMAQPQRAAVALIGDGGFGSNPSVVATA
CCCCCCEECCCCEECCCCCEECCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCEEEEE
MEAELAVVWLVMDNSAYGTIAGLEKAHYGTSFGCLFERQGRPYSVDYAALARSYGAGGIR
EECCEEEEEEEECCCCCEEHHCCHHHCCCCCEEEEEECCCCCEECHHHHHHHHCCCCCEE
ISAADELGPALREALASGMPTVIQAEMENAPTPTPGHWNINDIYRRGD
EECHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]