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The map label for this gene is cysS [H]

Identifier: 116517189

GI number: 116517189

Start: 527164

End: 528507

Strand: Direct

Name: cysS [H]

Synonym: SPD_0515

Alternate gene names: 116517189

Gene position: 527164-528507 (Clockwise)

Preceding gene: 116515456

Following gene: 116515371

Centisome position: 25.76

GC content: 44.35

Gene sequence:

>1344_bases
ATGATTAAAATCTATGACACCATGTCTCGTGATTTGCGAGAATTTGTCCCGATTGAGGACGGCAAGATCAAGATGTATGT
TTGTGGGCCAACGGTGTACAACTATATCCACGTAGGAAACGCCCGTTCGACGGTAGCTTTTGATACGATTCGTCGCTATT
TTGAGTACCGTGGGTATAAGGTTGCCTATATTTCCAATTTTACAGATGTGGATGATAAGATTATCAACCGTGCCAGGGAA
GAAGGTATCACGCCTCAGGAGGTTGCGGATAAGTACATCGCTGCCTTTCGTGAGGATGTGACGGCCTTAGGCGTGAAACC
TGCGACTCGCCATCCGCGTGTAGTGGAGTTTATGGCAGACATCATCCGTTTTGTGGAAGACTTGATCGAGAGAGGCTTTG
CCTATGAGAGTCAAGGGGATGTCTATTTCCGTGTAGAAAAATCCCACAACTATGCTAAATTGGCTAATAAAACCTTGGAA
GATTTGGAGCTAGGTGCTTCAGGTCGTACCGATGAAGAAACGGCTCGTAAGGAAAATCCTGTAGATTTTGCTCTATGGAA
ATCTTCCAAACCAGGTGAGATTTCTTGGGATAGTCCTTGGGGACCTGGACGTCCGGGTTGGCATATTGAGTGTTCAGTCA
TGTCGACAGAGATTTTAGGTGATACCATTGATATCCACGGTGGTGGAGCTGACCTAGAGTTTCCACACCACACCAATGAA
ATTGCCCAATCTGAAGCTAAAACAGGCAAGGCTTTTGCTAACTACTGGATGCACAATGGCTTTGTCAATATCGACAATGT
CAAAATGTCTAAGTCCTTGGGTAACTTCATTACGGTACACGATGCCCTTAAAACTCTTGATGGGCAAGTACTTCGTTTCT
TCTTTGCGACCCAGCACTACCGTAAGCCTATCAACTTCACGGAAAAGGCAGTTCGCGATGCCGAGACCAATCTCAAGTAT
CTGAAGAATACTTACGAACAACCATTTACAGGAAACGTGGATGCCCAAGAGTTACAAAACTTTAAAGATAAGTTTGTAGC
AGCTATGGATGAAGATTTTAATGCTGCCAACGGTATCACAGTTGTCTTTGAAATGGCCAAATGGATCAACTCAGGGAACT
ATGATGCAAGTGTCAAGCAAGCTCTTGCAGATATGTTAGAAATTTTTGGAATTGTCTTTGTTGAGGAAGTTTTGGATGCA
GAGATTGAAGACTTGATTCAAAAACGCCAAGAGGCGCGTGCCAATCGAGACTTTGAGACAGCAGACCAAATCCGTGACCA
ATTGGTTACTCAAGGAATTAAGCTCCTTGATACCAAGGATGGAGTGAGGTGGACACGTGATTGA

Upstream 100 bases:

>100_bases
AAGGGATTCCGAAGTGTAGAGATATTCGGACTGAAATTTACTTGAACTTTTAGTGATGAAACTAATTGTTCTTGGATTTC
AGCTTTCCTGATTATGATTT

Downstream 100 bases:

>100_bases
TGTCAATCTCATTAACGGGATTGCGCTAGCCTTTGAGGGGGATGCGGTGTATTCTATGTATATTCGCCGTCACCTCATCC
TCAAAGGTATGACCAAACCC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARSTVAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRARE
EGITPQEVADKYIAAFREDVTALGVKPATRHPRVVEFMADIIRFVEDLIERGFAYESQGDVYFRVEKSHNYAKLANKTLE
DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEILGDTIDIHGGGADLEFPHHTNE
IAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNFITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKY
LKNTYEQPFTGNVDAQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIVFVEEVLDA
EIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD

Sequences:

>Translated_447_residues
MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARSTVAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRARE
EGITPQEVADKYIAAFREDVTALGVKPATRHPRVVEFMADIIRFVEDLIERGFAYESQGDVYFRVEKSHNYAKLANKTLE
DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEILGDTIDIHGGGADLEFPHHTNE
IAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNFITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKY
LKNTYEQPFTGNVDAQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIVFVEEVLDA
EIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD
>Mature_447_residues
MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARSTVAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRARE
EGITPQEVADKYIAAFREDVTALGVKPATRHPRVVEFMADIIRFVEDLIERGFAYESQGDVYFRVEKSHNYAKLANKTLE
DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEILGDTIDIHGGGADLEFPHHTNE
IAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNFITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKY
LKNTYEQPFTGNVDAQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIVFVEEVLDA
EIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI13375695, Length=491, Percent_Identity=34.2158859470468, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI21269877, Length=339, Percent_Identity=36.283185840708, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI62240992, Length=339, Percent_Identity=36.283185840708, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI10835051, Length=339, Percent_Identity=36.283185840708, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI303304987, Length=339, Percent_Identity=36.283185840708, Blast_Score=208, Evalue=7e-54,
Organism=Escherichia coli, GI1786737, Length=464, Percent_Identity=42.8879310344828, Blast_Score=355, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI17509795, Length=245, Percent_Identity=41.2244897959184, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17509797, Length=245, Percent_Identity=41.2244897959184, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324082, Length=232, Percent_Identity=46.1206896551724, Blast_Score=203, Evalue=6e-53,
Organism=Drosophila melanogaster, GI19922218, Length=506, Percent_Identity=32.6086956521739, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI19922364, Length=237, Percent_Identity=40.9282700421941, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e [H]

EC number: =6.1.1.16 [H]

Molecular weight: Translated: 51082; Mature: 51082

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARSTVAFDTIRRYFEYRGYK
CEEEHHHHHHHHHHHCCCCCCEEEEEEECCHHHHEEEECCCCCHHHHHHHHHHHHHCCEE
VAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTALGVKPATRHPRVVEFMAD
EEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
IIRFVEDLIERGFAYESQGDVYFRVEKSHNYAKLANKTLEDLELGASGRTDEETARKENP
HHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCC
VDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEILGDTIDIHGGGADLEFPHHTNE
CEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHCCCEEEECCCCCCCCCCCCHHH
IAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNFITVHDALKTLDGQVLRFFFATQHY
HHHHHHHHHHHHHHHHHHCCEEEECCEEHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHH
RKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVDAQELQNFKDKFVAAMDEDFNAANGIT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
VVFEMAKWINSGNYDASVKQALADMLEIFGIVFVEEVLDAEIEDLIQKRQEARANRDFET
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ADQIRDQLVTQGIKLLDTKDGVRWTRD
HHHHHHHHHHCCHHEEECCCCCCCCCC
>Mature Secondary Structure
MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARSTVAFDTIRRYFEYRGYK
CEEEHHHHHHHHHHHCCCCCCEEEEEEECCHHHHEEEECCCCCHHHHHHHHHHHHHCCEE
VAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTALGVKPATRHPRVVEFMAD
EEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
IIRFVEDLIERGFAYESQGDVYFRVEKSHNYAKLANKTLEDLELGASGRTDEETARKENP
HHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCC
VDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEILGDTIDIHGGGADLEFPHHTNE
CEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHCCCEEEECCCCCCCCCCCCHHH
IAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNFITVHDALKTLDGQVLRFFFATQHY
HHHHHHHHHHHHHHHHHHCCEEEECCEEHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHH
RKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVDAQELQNFKDKFVAAMDEDFNAANGIT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
VVFEMAKWINSGNYDASVKQALADMLEIFGIVFVEEVLDAEIEDLIQKRQEARANRDFET
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ADQIRDQLVTQGIKLLDTKDGVRWTRD
HHHHHHHHHHCCHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]