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The map label for this gene is gdhA [H]

Identifier: 116516965

GI number: 116516965

Start: 1187003

End: 1188349

Strand: Direct

Name: gdhA [H]

Synonym: SPD_1158

Alternate gene names: 116516965

Gene position: 1187003-1188349 (Clockwise)

Preceding gene: 116515764

Following gene: 116516155

Centisome position: 58.01

GC content: 42.91

Gene sequence:

>1347_bases
ATGACATCTGCTAAAGAATATATCCAAAGCGTGTTTGAAACTGTAAAAGCTCGTAACGGGCACGAGGCTGAATTCCTCCA
AGCTGTTGAAGAATTTTTCAACACTTTGGAACCTGTATTTGAAAAACACCCTGAGTATATCGAAGAAAATATCTTGGCAC
GTATTACTGAGCCTGAGCGCGTGATTTCTTTCCGTGTTCCTTGGGTTGACCGTGATGGAAAAGTTCAAGTAAACCGCGGC
TACCGTGTTCAATTCAACTCAGCTGTTGGACCATATAAAGGCGGACTTCGTTTCCACCCAACTGTAAACCAAGGGATTTT
GAAATTCCTCGGATTTGAACAAATCTTTAAAAACGTCTTGACTGGACTTCCTATCGGTGGAGGTAAAGGTGGATCAGACT
TCGATCCTAAAGGTAAAACAGATGCTGAAGTGATGCGCTTCTGCCAAAGCTTCATGACTGAATTGCAAAAACACATTGGA
CCATCACTTGACGTACCTGCTGGTGATATCGGTGTTGGTGGACGTGAGATTGGTTACCTTTACGGTCAATACAAGCGTCT
TAACCAATTTGATGCTGGTGTCTTGACTGGTAAACCTCTTGGATTTGGTGGTAGCTTGATTCGTCCAGAAGCAACTGGTT
ACGGTTTGGTTTACTATACTGAAGAAATGCTCAAAGCTAACGGTAACAGCTTTGCTGGTAAGAAAGTGGTTATTTCAGGT
TCTGGTAACGTTGCTCAATACGCTCTTCAAAAAGCAACCGAACTTGGTGCAACAGTTATCTCTGTTTCTGACTCAAACGG
CTATGTTATCGATGAAAACGGTATCGACTTCGATCTTTTGGTTGATGTTAAAGAAAAACGTCGCGCTCGTTTGACTGAGT
ATGCAGCTGAGAAAGCAACTGCTACTTATCATGAAGGTTCTGTATGGACTTACGCTGGAAACTATGACATTGCGCTTCCA
TGTGCTACTCAAAACGAAATCAACGGTGAAGCAGCTAAACGTTTGGTTGCTCAAGGCGTTATCTGTGTATCTGAAGGTGC
CAACATGCCAAGCAACCTTGATGCCATCAAAGTTTACAAAGAAAATGGTATCTTCTACGGACCTGCCAAAGCTGCCAACG
CTGGTGGTGTAGCCGTTTCAGCCCTTGAAATGAGCCAAAACAGCCTTCGCCTCTCATGGACTCGTGAAGAAGTTGATGGA
CGTCTCAAAGACATCATGACCAACATCTTTAACACAGCTAAAACAACTTCAGAAACATATGGTCTTGATAAAGACTACCT
TGCAGGAGCTAACATTGCTGCCTTTGAAAATGTAGCAAACGCTATGATTGCACAAGGTATTGTTTAA

Upstream 100 bases:

>100_bases
TGAAAGAATTTTTAGAAAATTTCTGTTTTTTCTTTACAGTGAAAAAAAATTCTGCTATAATGTCTTATGTAATAAAATAT
GACAATAAAAGGAGAACGAT

Downstream 100 bases:

>100_bases
GATTTATTTGCTTAATCCTCAATCACTACGATTGGGGATTTTTAGTTGAGTTAGACAGTTGGGAAATTCTAATTAAATAA
AATAAAAACTGAACAAATTA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPERVISFRVPWVDRDGKVQVNRG
YRVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISG
SGNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALP
CATQNEINGEAAKRLVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDG
RLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV

Sequences:

>Translated_448_residues
MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPERVISFRVPWVDRDGKVQVNRG
YRVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISG
SGNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALP
CATQNEINGEAAKRLVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDG
RLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV
>Mature_447_residues
TSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPERVISFRVPWVDRDGKVQVNRGY
RVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIGP
SLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISGS
GNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPC
ATQNEINGEAAKRLVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDGR
LKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI4885281, Length=398, Percent_Identity=29.6482412060301, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI31377775, Length=412, Percent_Identity=29.3689320388349, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1788059, Length=444, Percent_Identity=55.1801801801802, Blast_Score=503, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17544676, Length=360, Percent_Identity=29.1666666666667, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319256, Length=455, Percent_Identity=53.4065934065934, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6324951, Length=453, Percent_Identity=53.4216335540839, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24649283, Length=407, Percent_Identity=29.2383292383292, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45549226, Length=359, Percent_Identity=31.7548746518106, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI45553475, Length=359, Percent_Identity=31.7548746518106, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24649460, Length=294, Percent_Identity=32.6530612244898, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48797; Mature: 48665

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPER
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCC
VISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVL
EEEEECCEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHHH
TGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIGPSLDVPAGDIGVGGREIGYL
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
YGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISG
HHHHHHHHHCCCCEECCCCCCCCCCEECCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEEC
SGNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKAT
CCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHH
ATYHEGSVWTYAGNYDIALPCATQNEINGEAAKRLVAQGVICVSEGANMPSNLDAIKVYK
CEEECCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEE
ENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDGRLKDIMTNIFNTAKTTSETY
CCCEEECCCCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHC
GLDKDYLAGANIAAFENVANAMIAQGIV
CCCHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPER
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCC
VISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVL
EEEEECCEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHHH
TGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIGPSLDVPAGDIGVGGREIGYL
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
YGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISG
HHHHHHHHHCCCCEECCCCCCCCCCEECCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEEC
SGNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKAT
CCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHH
ATYHEGSVWTYAGNYDIALPCATQNEINGEAAKRLVAQGVICVSEGANMPSNLDAIKVYK
CEEECCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEE
ENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDGRLKDIMTNIFNTAKTTSETY
CCCEEECCCCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHC
GLDKDYLAGANIAAFENVANAMIAQGIV
CCCHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]