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The map label for this gene is trmD

Identifier: 116516895

GI number: 116516895

Start: 696448

End: 697167

Strand: Direct

Name: trmD

Synonym: SPD_0679

Alternate gene names: 116516895

Gene position: 696448-697167 (Clockwise)

Preceding gene: 116516249

Following gene: 116515795

Centisome position: 34.04

GC content: 42.92

Gene sequence:

>720_bases
ATGAAGATTGATATTTTAACCCTCTTTCCAGAGATGTTTTCTCCACTGGAGCACTCAATCGTTGGAAAGGCTCGAGAAAA
AGGGCTCTTGGATATCCAGTATCATAATTTTCGAGAAAATGCTGAAAAGGCCCGTCATGTAGATGATGAGCCCTACGGAG
GCGGTCAGGGCATGTTGCTCAGAGTACAACCTATTTTCGATTCCTTTGATGCTATTGAAAAGAAAAATCCGCGCGTTATT
CTCCTCGATCCTGCTGGAAAGCAGTTTGATCAGGCTTATGCTGAAGATTTGGCTCAAGAGGAAGAGCTAATCTTTATCTG
TGGGCACTATGAGGGTTATGATGAGCGCATTAAGACCTTGGTAACAGATGAGATTTCCCTAGGCGACTATGTTCTCACTG
GTGGAGAATTGGCAGCTATGACCATGATTGATGCTACAGTTCGCCTGATTCCAGAAGTGATTGGCAAGGAGTCTAGCCAC
CAAGATGATAGTTTTTCTTCAGGTCTTTTAGAATATCCTCAGTACACACGTCCCTATGATTATCGAGGCATGGTCGTGCC
AGATGTATTGATGAGTGGCCACCATGAAAAGATTCGTCAGTGGCGATTGTACGAGAGTTTAAAGAAAACCTACGAGCGCA
GACCGGATTTACTTGAACATTATCAACTGACAGTAGAAGAAGAAAAAATGCTGGCAGAAATCAAAGAAAACAAAGAATAA

Upstream 100 bases:

>100_bases
GGCAAACGTGATTTGCTTTTACCTTATATCCCACCAGTGGTTCTCAATGTTGATATTCCAAATAAACGGGTCGATGTGGA
AATCTTAGAAGGGTTAGACG

Downstream 100 bases:

>100_bases
AGGAGAAACCTATGCAAGTAATCAAACGTAATGGCGAAATTGCTGAATTTAATCCAGATAAGATTTACCAAGCCATCTTG
AAGGCAGCCCAAACTGTTTA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKARHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPRVI
LLDPAGKQFDQAYAEDLAQEEELIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLLEHYQLTVEEEKMLAEIKENKE

Sequences:

>Translated_239_residues
MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKARHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPRVI
LLDPAGKQFDQAYAEDLAQEEELIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLLEHYQLTVEEEKMLAEIKENKE
>Mature_239_residues
MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKARHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPRVI
LLDPAGKQFDQAYAEDLAQEEELIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLLEHYQLTVEEEKMLAEIKENKE

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=49.792531120332, Blast_Score=227, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_STRP2 (Q04LC5)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816173.1
- ProteinModelPortal:   Q04LC5
- SMR:   Q04LC5
- STRING:   Q04LC5
- EnsemblBacteria:   EBSTRT00000020656
- GeneID:   4442080
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0679
- eggNOG:   COG0336
- GeneTree:   EBGT00050000027798
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27662; Mature: 27662

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKARHVDDEPYGGGQGMLL
CEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCCCCCCCCCEEEE
RVQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEELIFICGHYEGYDERIKTL
EEECHHCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
VTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSHQDDSFSSGLLEYPQYTRPYD
HHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCC
YRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLLEHYQLTVEEEKMLAEIKENKE
CCCEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKARHVDDEPYGGGQGMLL
CEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCCCCCCCCCEEEE
RVQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEELIFICGHYEGYDERIKTL
EEECHHCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
VTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSHQDDSFSSGLLEYPQYTRPYD
HHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCC
YRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLLEHYQLTVEEEKMLAEIKENKE
CCCEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA