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The map label for this gene is gidB

Identifier: 116516833

GI number: 116516833

Start: 1173732

End: 1174445

Strand: Direct

Name: gidB

Synonym: SPD_1140

Alternate gene names: 116516833

Gene position: 1173732-1174445 (Clockwise)

Preceding gene: 116517191

Following gene: 116516034

Centisome position: 57.36

GC content: 41.88

Gene sequence:

>714_bases
ATGAAACCAGAAACATTTTACAACTTGCTTGCCGAGCAGAATCTTCCACTTTCGAACCAGCAAAAAGAACAATTTGAACG
TTATTTTGAGCTCTTGGTCGAGTGGAATGAGAAGATTAATTTGACGGCGATTACGGACAAGGAAGAAGTTTATCTCAAAC
ATTTTTACGATTCGATTGCACCCATTCTTCAAGGTTTGATTCCCAATGAAACTATCAAACTTCTTGATATCGGGGCTGGG
GCAGGATTTCCTAGTCTACCAATGAAAATTCTCTATCCGGAGTTAGATGTGACCATTATTGATTCACTCAATAAGCGCAT
CAACTTCCTACAACTCTTGGCTCAAGAACTGGATTTGAACGGAGTTCATTTCTACCACGGACGTGCCGAAGATTTTGCCC
AAGACAAGAACTTCCGTGCTCAATATGATTTTGTAACAGCTCGTGCGGTTGCCCGTATGCAGGTCCTATCTGAATTGACT
ATTCCCTACCTTAAGGTTGGTGGAAAACTATTGGCACTCAAGGCCAGCAATGCGCCTGAGGAGTTGTTAGAAGCTAAGAA
TGCCCTCAATCTCCTTTTTAGTAAAGTCGAAGACAATCTCAGCTACGCCCTACCAAATAGAGATCCGCGTTACATCACAG
TGGTAGAAAAGAAAAAAGAAACACCAAATAAATATCCACGTAAGGCTGGTATGCCAAATAAACGCCCGCTTTAA

Upstream 100 bases:

>100_bases
AAGTCATGATATAAGTCCTTTCTACTTTTAGATTAGTACCAGTATATCAAATTTTTAATGATTGTGGTAAAATAAGATGA
TACTAAAGAAGGAAATAACT

Downstream 100 bases:

>100_bases
ATTTTTAAGTAAAAAAATATTTACAAAATCAACCTCGCTCTCTTATTTTTAGGCTCGGGAAAAAATGATTTACAAAATCA
GCCTCGCTCTTTTATTTCTA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MKPETFYNLLAEQNLPLSNQQKEQFERYFELLVEWNEKINLTAITDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAG
AGFPSLPMKILYPELDVTIIDSLNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKKKETPNKYPRKAGMPNKRPL

Sequences:

>Translated_237_residues
MKPETFYNLLAEQNLPLSNQQKEQFERYFELLVEWNEKINLTAITDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAG
AGFPSLPMKILYPELDVTIIDSLNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKKKETPNKYPRKAGMPNKRPL
>Mature_237_residues
MKPETFYNLLAEQNLPLSNQQKEQFERYFELLVEWNEKINLTAITDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAG
AGFPSLPMKILYPELDVTIIDSLNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKKKETPNKYPRKAGMPNKRPL

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=176, Percent_Identity=32.9545454545455, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_STRP2 (Q04K39)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816609.1
- ProteinModelPortal:   Q04K39
- SMR:   Q04K39
- STRING:   Q04K39
- EnsemblBacteria:   EBSTRT00000019612
- GeneID:   4442151
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1140
- eggNOG:   COG0357
- GeneTree:   EBGT00050000027008
- HOGENOM:   HBG686577
- OMA:   YELLVEW
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 27199; Mature: 27199

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPETFYNLLAEQNLPLSNQQKEQFERYFELLVEWNEKINLTAITDKEEVYLKHFYDSIA
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHH
PILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDSLNKRINFLQLLAQELDLN
HHHHCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELTIPYLKVGGKLLALKASNAPE
CEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCHH
ELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKKKETPNKYPRKAGMPNKRPL
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure
MKPETFYNLLAEQNLPLSNQQKEQFERYFELLVEWNEKINLTAITDKEEVYLKHFYDSIA
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHH
PILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDSLNKRINFLQLLAQELDLN
HHHHCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELTIPYLKVGGKLLALKASNAPE
CEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCHH
ELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKKKETPNKYPRKAGMPNKRPL
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA