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The map label for this gene is proB

Identifier: 116516764

GI number: 116516764

Start: 840495

End: 841604

Strand: Direct

Name: proB

Synonym: SPD_0822

Alternate gene names: 116516764

Gene position: 840495-841604 (Clockwise)

Preceding gene: 116516225

Following gene: 116516154

Centisome position: 41.08

GC content: 44.14

Gene sequence:

>1110_bases
ATGAAATACAAACGGATTGTCTTTAAGGTGGGTACTTCTTCTCTGACAAATGAGGATGGAAGTTTATCACGTAGTAAGGT
AAAGGATATTACCCAGCAGTTGGCTATGCTGCACGAGGCTGGTCATGAGTTGATTTTGGTGTCTTCAGGTGCCATTGCGG
CTGGTTTTGGAGCCTTAGGATTTAAAAAGCGTCCGACTAAAATTGCTGATAAACAGGCTTCAGCAGCGGTAGGGCAGGGA
CTTTTGTTGGAAGAATATACGACCAATCTTCTCTTGCGTCAAATCGTTTCTGCACAAATCTTGCTGACCCAAGATGACTT
TGTGGATAAGCGTCGTTATAAAAATGCCCATCAGGCTTTGTCGGTTTTGCTCAACCGTGGGGCAATTCCTATCATCAATG
AGAATGATAGTGTCGTTATTGATGAGCTCAAGGTTGGGGACAATGACACTCTAAGTGCTCAAGTAGCGGCGATGGTCCAA
GCAGACCTTTTAGTTTTCTTGACAGATGTGGACGGTCTCTATACTGGAAATCCTAATTCAGATCCAAGAGCCAAACGCTT
GGAGAGAATCGAGACCATCAATCGTGAGATTATTGATATGGCTGGTGGAGCTGGTTCGTCAAACGGAACTGGGGGTATGT
TAACCAAAATCAAGGCTGCAACTATCGCGACGGAATCAGGAGTTCCTGTTTATATCTGCTCATCCTTGAAATCAGATTCC
ATGATTGAGGCGGCAGAGGAGACCGAGGATGGTTCTTACTTTGTTGCTCAAGAGAAGGGGCTTCGTACCCAGAAACAATG
GCTTGCCTTCTATGCTCAGAGTCAAGGTTCTATTTGGGTTGATAAAGGGGCTGCGGAAGCTCTCTCTCAACATGGAAAGA
GTCTTCTCTTATCTGGTATCGTTGAAGCAGAAGGAGTCTTTTCTTACGGTGATATCGTGACAGTATTTGATAAGGAAAGT
GGAAAATCACTTGGAAAAGGACGCGTGCAATTTGGAGCATCTGCTTTGGAGGATATATTGCGTTCTCAAAAAGCCAAGGG
TGTCTTGATTTACCGTGACGACTGGATTTCCATTACTCCTGAAATCCAACTACTTTTTACAGAATTTTAG

Upstream 100 bases:

>100_bases
TAGAAAAGAAGGTTTTAGGGCCTTCTTTTTCCTATCAACTCTTTTCTATTTCCTGTTATTCATGTTATAATGGATAAATA
TGAATAATCGGAGTGAGACT

Downstream 100 bases:

>100_bases
AGGTAAACTATGGTGAGTAGACAAGAACAATTTGAACAGGTACAGGCTGTTAAAAAATCGATTAACACAGCTAGTGAAGA
AGTGAAAAACCAAGCCTTGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQG
LLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQ
ADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICSSLKSDS
MIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKES
GKSLGKGRVQFGASALEDILRSQKAKGVLIYRDDWISITPEIQLLFTEF

Sequences:

>Translated_369_residues
MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQG
LLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQ
ADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICSSLKSDS
MIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKES
GKSLGKGRVQFGASALEDILRSQKAKGVLIYRDDWISITPEIQLLFTEF
>Mature_369_residues
MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQG
LLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQ
ADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICSSLKSDS
MIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKES
GKSLGKGRVQFGASALEDILRSQKAKGVLIYRDDWISITPEIQLLFTEF

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=260, Percent_Identity=32.3076923076923, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI62912457, Length=258, Percent_Identity=32.5581395348837, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1786437, Length=362, Percent_Identity=38.3977900552486, Blast_Score=232, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17569967, Length=270, Percent_Identity=34.0740740740741, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17569969, Length=270, Percent_Identity=34.0740740740741, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320506, Length=410, Percent_Identity=34.6341463414634, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321822, Length=268, Percent_Identity=38.8059701492537, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI21357643, Length=287, Percent_Identity=33.4494773519164, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_STRP2 (Q04KZ3)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816305.1
- ProteinModelPortal:   Q04KZ3
- SMR:   Q04KZ3
- STRING:   Q04KZ3
- EnsemblBacteria:   EBSTRT00000020255
- GeneID:   4442275
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0822
- eggNOG:   COG0263
- GeneTree:   EBGT00050000027969
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39884; Mature: 39884

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALG
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCC
FKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQAL
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHH
SVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQADLLVFLTDVDGLYTGNPNS
HHHHHCCCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCEEEEEECCCCEECCCCCC
DPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICSSLKSDS
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEEEECCCCCEEEECCCCCCC
MIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWVDKGAAEALSQHGKSLLLSGI
HHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHCCHHHHHHHH
VEAEGVFSYGDIVTVFDKESGKSLGKGRVQFGASALEDILRSQKAKGVLIYRDDWISITP
HHHCCEEECCCEEEEEECCCCCCCCCCHHHCCHHHHHHHHHHHCCCEEEEEECCCEEECC
EIQLLFTEF
CEEEEEECC
>Mature Secondary Structure
MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALG
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCC
FKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQAL
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHH
SVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQADLLVFLTDVDGLYTGNPNS
HHHHHCCCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCEEEEEECCCCEECCCCCC
DPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICSSLKSDS
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEEEECCCCCEEEECCCCCCC
MIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWVDKGAAEALSQHGKSLLLSGI
HHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHCCHHHHHHHH
VEAEGVFSYGDIVTVFDKESGKSLGKGRVQFGASALEDILRSQKAKGVLIYRDDWISITP
HHHCCEEECCCEEEEEECCCCCCCCCCHHHCCHHHHHHHHHHHCCCEEEEEECCCEEECC
EIQLLFTEF
CEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA