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The map label for this gene is gmk [H]

Identifier: 116516658

GI number: 116516658

Start: 1571421

End: 1572047

Strand: Reverse

Name: gmk [H]

Synonym: SPD_1548

Alternate gene names: 116516658

Gene position: 1572047-1571421 (Counterclockwise)

Preceding gene: 116516869

Following gene: 116515579

Centisome position: 76.83

GC content: 44.02

Gene sequence:

>627_bases
ATGGCAGACCGAGGCTTACTAATCGTTTTTTCTGGTCCTTCAGGGGTTGGAAAAGGAACGGTTAGAAGAGAGATTTTTGA
GAGTTCTGAAAACCAATTTCAATATTCTGTATCGATGACGACACGCGCACAACGTCCTGGAGAAGTGGACGGTGTTGACT
ATTTCTTCCGTACTCGTGAAGAATTTGAAGAGCTGATTCGTCAAGGACAGATGTTGGAATACGCAGAATATGTCGGTAAC
TACTATGGAACTCCTCTGACCTATGTCAATGAAACCTTGGACAAGGGAATCGATGTTTTCCTTGAAATTGAAGTTCAGGG
TGCTCTTCAGGTCAAGAAAAAGGTTCCAGATGCTGTCTTTATCTTCCTGACACCACCAGATTTGGATGAATTGCAAGATC
GCTTGGTAGGTCGTGGAACAGATAGTGCAGAAGTGATTGCCCAACGAATCGAAAAGGCCAAGGAAGAAATTGCCCTCATG
CGTGAGTATGATTATGCCATTGTCAACGATCAGGTGTCCCTAGCTGCTGAACGTGTCAAATGTGTGATTGAAGCAGAACA
CTTCTGTGTGGATCGTGTCATTGGTCACTATCAGGAGATGTTACCAAAATCTCCAACTACCCGATAA

Upstream 100 bases:

>100_bases
TTTTTTGTTACACTAGATAGAAAGACTGTAAAGGATAATCTGGCTTTTTGCCATTATTCTAGAGAAATGTTATTTCACAG
ATTGACTAAAAGGAGACACA

Downstream 100 bases:

>100_bases
AATTTAGAAAATAGGTACAAGCAAATGATGTTAAAACCCTCTATTGATACCTTGCTCGACAAGGTTCCTTCAAAATATTC
ACTCGTAATCTTGGAAGCAA

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQRPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGN
YYGTPLTYVNETLDKGIDVFLEIEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
REYDYAIVNDQVSLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR

Sequences:

>Translated_208_residues
MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQRPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGN
YYGTPLTYVNETLDKGIDVFLEIEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
REYDYAIVNDQVSLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR
>Mature_207_residues
ADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQRPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNY
YGTPLTYVNETLDKGIDVFLEIEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALMR
EYDYAIVNDQVSLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR

Specific function: Essential for recycling GMP and indirectly, cGMP [H]

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain [H]

Homologues:

Organism=Homo sapiens, GI226874833, Length=182, Percent_Identity=34.0659340659341, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI4504221, Length=182, Percent_Identity=34.0659340659341, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI226874831, Length=182, Percent_Identity=34.0659340659341, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI14780902, Length=198, Percent_Identity=30.3030303030303, Blast_Score=91, Evalue=6e-19,
Organism=Homo sapiens, GI38570142, Length=176, Percent_Identity=30.1136363636364, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI21361598, Length=187, Percent_Identity=28.8770053475936, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI111154074, Length=129, Percent_Identity=28.6821705426357, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI4505237, Length=167, Percent_Identity=28.1437125748503, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI262118259, Length=167, Percent_Identity=28.1437125748503, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI262118255, Length=167, Percent_Identity=28.1437125748503, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI262118263, Length=167, Percent_Identity=28.1437125748503, Blast_Score=68, Evalue=5e-12,
Organism=Escherichia coli, GI1790080, Length=201, Percent_Identity=43.2835820895522, Blast_Score=162, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17508835, Length=172, Percent_Identity=37.2093023255814, Blast_Score=120, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI193203132, Length=172, Percent_Identity=37.2093023255814, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17556244, Length=121, Percent_Identity=35.5371900826446, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71988041, Length=127, Percent_Identity=32.2834645669291, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71988048, Length=127, Percent_Identity=32.2834645669291, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320662, Length=183, Percent_Identity=39.8907103825137, Blast_Score=138, Evalue=6e-34,
Organism=Drosophila melanogaster, GI21356141, Length=150, Percent_Identity=38, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161077626, Length=176, Percent_Identity=30.6818181818182, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45554489, Length=176, Percent_Identity=30.6818181818182, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640542, Length=176, Percent_Identity=30.6818181818182, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI85724806, Length=176, Percent_Identity=30.6818181818182, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI116007136, Length=176, Percent_Identity=30.6818181818182, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45554481, Length=171, Percent_Identity=30.9941520467836, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221330166, Length=125, Percent_Identity=32.8, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI221330162, Length=125, Percent_Identity=32.8, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI85816281, Length=127, Percent_Identity=31.496062992126, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI85816106, Length=127, Percent_Identity=31.496062992126, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45551002, Length=127, Percent_Identity=31.496062992126, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI221330164, Length=125, Percent_Identity=32.8, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665 [H]

Pfam domain/function: PF00625 Guanylate_kin [H]

EC number: =2.7.4.8 [H]

Molecular weight: Translated: 23676; Mature: 23545

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00856 GUANYLATE_KINASE_1 ; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQRPGEVDGVDYFFRTRE
CCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHH
EFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLEIEVQGALQVKKKVPDAVF
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCCEEE
IFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALMREYDYAIVNDQVSLAAERVK
EEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHH
CVIEAEHFCVDRVIGHYQEMLPKSPTTR
HHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQRPGEVDGVDYFFRTRE
CCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHH
EFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLEIEVQGALQVKKKVPDAVF
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCCEEE
IFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALMREYDYAIVNDQVSLAAERVK
EEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHH
CVIEAEHFCVDRVIGHYQEMLPKSPTTR
HHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]