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The map label for this gene is topA [H]

Identifier: 116516615

GI number: 116516615

Start: 1149238

End: 1151325

Strand: Reverse

Name: topA [H]

Synonym: SPD_1120

Alternate gene names: 116516615

Gene position: 1151325-1149238 (Counterclockwise)

Preceding gene: 116515556

Following gene: 116515673

Centisome position: 56.27

GC content: 42.58

Gene sequence:

>2088_bases
GTGGCTACGGCAACAAAAAAGAAAAAATCAACAGTTAAAAAAAATCTAGTCATCGTGGAGTCGCCTGCTAAGGCCAAGAC
GATTGAAAAATATCTAGGCAGAAACTACAAGGTTTTAGCCAGTGTCGGGCATATCCGTGATTTGAAGAAATCCAGTATGT
CCGTCGATATTGAAAATAATTATGAACCGCAATATATTAATATCCGAGGAAAAGGCCCTCTTATCAATGACTTGAAAAAA
GAAGCTAAAAAAGCTAATAAAGTTTTTCTCGCGAGTGACCCGGACCGTGAAGGAGAAGCGATTTCTTGGCATTTGGCCCA
TATTCTCAACTTGGATGAAAATGATGCCAACCGTGTGGTCTTCAATGAAATCACCAAGGATGCAGTCAAAAATGCTTTTA
AAGAACCTCGTAAGATCGATATGGACTTGGTCGATGCCCAACAAGCTCGTCGGATCTTGGATCGCTTGGTAGGGTATTCG
ATTTCGCCTATTTTGTGGAAGAAGGTCAAGAAGGGCTTGTCAGCAGGTCGCGTTCAGTCCATTGCCCTTAAACTCATCAT
TGACCGTGAAAATGAAATCAATGCCTTCCAGCCAGAAGAATACTGGACAGTTGATGCTGTCTTTAAAAAGGGAACCAAAC
AATTTCATGCTTCCTTCTATGGAGTAGATGGTAAAAAGATGAAACTGACCAGCAATAACGAAGTCAAGGAAGTCTTGTCT
CGTCTGACGAGTAAAGACTTTTCAGTAGATCAGGTGGATAAGAAAGAGCGTAAGCGCAATGCTCCTTTACCCTATACCAC
TTCATCTATGCAGATGGATGCTGCCAATAAAATCAATTTCCGTACTCGAAAAACCATGATGGTTGCCCAACAGCTCTATG
AAGGAATTAATATCGGTTCTGGTGTTCAAGGTTTGATTACCTATATGCGTACCGATTCGACTCGTATCAGTCCTGTAGCG
CAAAATGAGGCGGCAAGCTTCATTACGGATCGTTTTGGTAGCAAGTATTCTAAGCACGGTAGCAAGGTCAAAAACGCATC
AGGTGCTCAGGATGCCCATGAGGCTATTCGTCCGTCAAGTGTCTTTAATACACCAGAAAGCATCGCTAAGTATCTGGACA
AGGATCAGCTCAAGCTATATACCCTTATCTGGAATCGTTTTGTGGCTAGCCAGATGACAGCGGCCGTTTTTGATACCATG
GCTGTTAAATTGTCTCAAAAAGGGGTTCAATTTGCTGCCAATGGTAGTCAGGTTAAGTTTGATGGTTATCTTGCCATTTA
TAATGATTCTGACAAGAATAAGATGTTACCGGACATGGTTGTTGGAGATGTGGTCAAACAGGTCAATAGCAAACCAGAGC
AACATTTCACCCAACCGCCTGCCCGTTATTCTGAAGCAACACTGATTAAAACCTTAGAGGAAAATGGGGTTGGACGTCCA
TCAACCTACGCGCCAACCATTGAAACCATTCAGAAACGTTATTATGTTCGCCTGGCAGCCAAACGTTTTGAACCGACAGA
GTTGGGAGAAATTGTCAATAAGCTCATCGTTGAATATTTCCCAGATATCGTAAACGTGACCTTCACAGCTGAAATGGAAG
GTAAACTGGATGATGTCGAAGTTGGAAAAGAGCAGTGGCGACGGGTCATTGATGCCTTTTACAAACCATTCTCTAAAGAA
GTTGCCAAGGCTGAAGAAGAAATGGAAAAAATCCAGATTAAGGATGAACCAGCTGGATTTGACTGTGAAGTGTGTGGTAG
TCCAATGGTCATTAAACTTGGTCGTTTTGGTAAATTCTACGCTTGTAGCAATTTCCCAGATTGCCATCATACCCAAGCAA
TCGTGAAAGAGATTGGTGTTGAGTGTCCAAGCTGTCATCAGGGACAAATTATTGAGCGAAAAACCAAGCGTAATCGCCTA
TTCTATGGTTGCAATCGCTATCCAGAATGTGAATTTACCTCTTGGGACAAGCCTGTTGGTCGTGACTGTCCAAAATGTGG
CAACTTCCTCATGGAGAAAAAAGTCCGTGGTGGTGGCAAGCAGGTTGTTTGTAGCAAAGGCGACTACGAGGAAGAAAAGA
TTAAATAA

Upstream 100 bases:

>100_bases
AGTATAAAACTTTTCTTCTATTATAGTAGCTAAGTCTTGACAGGGTTCAAAAAATGGTTTACACTTTATAAAGTTTATTA
CTTTGAAAAGGTGTGATACT

Downstream 100 bases:

>100_bases
GAGGAGGGGCCCGAAAGTAAATTTCAGGCTCCTTTTGGTTAGAACTTGACAAAATACATCATTTTATGCGAAACTAGAAG
AAGATTATTTTAACTAGGAG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGHIRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKK
EAKKANKVFLASDPDREGEAISWHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHASFYGVDGKKMKLTSNNEVKEVLS
RLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAANKINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVA
QNEAASFITDRFGSKYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQMTAAVFDTM
AVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNSKPEQHFTQPPARYSEATLIKTLEENGVGRP
STYAPTIETIQKRYYVRLAAKRFEPTELGEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKE
VAKAEEEMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCHHTQAIVKEIGVECPSCHQGQIIERKTKRNRL
FYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQVVCSKGDYEEEKIK

Sequences:

>Translated_695_residues
MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGHIRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKK
EAKKANKVFLASDPDREGEAISWHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHASFYGVDGKKMKLTSNNEVKEVLS
RLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAANKINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVA
QNEAASFITDRFGSKYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQMTAAVFDTM
AVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNSKPEQHFTQPPARYSEATLIKTLEENGVGRP
STYAPTIETIQKRYYVRLAAKRFEPTELGEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKE
VAKAEEEMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCHHTQAIVKEIGVECPSCHQGQIIERKTKRNRL
FYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQVVCSKGDYEEEKIK
>Mature_694_residues
ATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGHIRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKE
AKKANKVFLASDPDREGEAISWHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYSI
SPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHASFYGVDGKKMKLTSNNEVKEVLSR
LTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAANKINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQ
NEAASFITDRFGSKYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQMTAAVFDTMA
VKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNSKPEQHFTQPPARYSEATLIKTLEENGVGRPS
TYAPTIETIQKRYYVRLAAKRFEPTELGEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEV
AKAEEEMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCHHTQAIVKEIGVECPSCHQGQIIERKTKRNRLF
YGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQVVCSKGDYEEEKIK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=617, Percent_Identity=25.9319286871961, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI4507635, Length=530, Percent_Identity=23.0188679245283, Blast_Score=109, Evalue=1e-23,
Organism=Escherichia coli, GI1787529, Length=745, Percent_Identity=38.5234899328859, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI48994931, Length=108, Percent_Identity=40.7407407407407, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1788061, Length=514, Percent_Identity=22.7626459143969, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32563869, Length=650, Percent_Identity=25.8461538461538, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=452, Percent_Identity=25.2212389380531, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=493, Percent_Identity=23.3265720081136, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24585251, Length=628, Percent_Identity=22.7707006369427, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24640096, Length=505, Percent_Identity=22.7722772277228, Blast_Score=101, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 78664; Mature: 78533

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGHIRDLKKSSMSVDIENN
CCCCCHHHHHHHHHCEEEEECCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCEEEECCC
YEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAISWHLAHILNLDENDANRVV
CCCCEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEHHHCCCCCCCCCCHH
FNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYSISPILWKKVKKGLSAGRVQS
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHH
IALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHASFYGVDGKKMKLTSNNEVKEVLS
EEEEEEEECCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHH
RLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAANKINFRTRKTMMVAQQLYEGINIGS
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCC
GVQGLITYMRTDSTRISPVAQNEAASFITDRFGSKYSKHGSKVKNASGAQDAHEAIRPSS
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHCCCHH
VFNTPESIAKYLDKDQLKLYTLIWNRFVASQMTAAVFDTMAVKLSQKGVQFAANGSQVKF
HCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEE
DGYLAIYNDSDKNKMLPDMVVGDVVKQVNSKPEQHFTQPPARYSEATLIKTLEENGVGRP
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCC
STYAPTIETIQKRYYVRLAAKRFEPTELGEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVE
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHEEEEEEECCCCCCHHH
VGKEQWRRVIDAFYKPFSKEVAKAEEEMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFY
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEECCCCCCCCHHCCCCCEEEEECCCCCEE
ACSNFPDCHHTQAIVKEIGVECPSCHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVG
EECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCC
RDCPKCGNFLMEKKVRGGGKQVVCSKGDYEEEKIK
CCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHCCC
>Mature Secondary Structure 
ATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGHIRDLKKSSMSVDIENN
CCCCHHHHHHHHHCEEEEECCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCEEEECCC
YEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAISWHLAHILNLDENDANRVV
CCCCEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEHHHCCCCCCCCCCHH
FNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYSISPILWKKVKKGLSAGRVQS
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHH
IALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHASFYGVDGKKMKLTSNNEVKEVLS
EEEEEEEECCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHH
RLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAANKINFRTRKTMMVAQQLYEGINIGS
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCC
GVQGLITYMRTDSTRISPVAQNEAASFITDRFGSKYSKHGSKVKNASGAQDAHEAIRPSS
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHCCCHH
VFNTPESIAKYLDKDQLKLYTLIWNRFVASQMTAAVFDTMAVKLSQKGVQFAANGSQVKF
HCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEE
DGYLAIYNDSDKNKMLPDMVVGDVVKQVNSKPEQHFTQPPARYSEATLIKTLEENGVGRP
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCC
STYAPTIETIQKRYYVRLAAKRFEPTELGEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVE
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHEEEEEEECCCCCCHHH
VGKEQWRRVIDAFYKPFSKEVAKAEEEMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFY
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEECCCCCCCCHHCCCCCEEEEECCCCCEE
ACSNFPDCHHTQAIVKEIGVECPSCHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVG
EECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCCCC
RDCPKCGNFLMEKKVRGGGKQVVCSKGDYEEEKIK
CCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]