Click here to switch to the map view.

The map label for this gene is thiN [H]

Identifier: 116516589

GI number: 116516589

Start: 1775644

End: 1776306

Strand: Reverse

Name: thiN [H]

Synonym: SPD_1779

Alternate gene names: 116516589

Gene position: 1776306-1775644 (Counterclockwise)

Preceding gene: 116515540

Following gene: 116516808

Centisome position: 86.81

GC content: 45.1

Gene sequence:

>663_bases
ATGAGCGAGTACAAACTCTCAGAAAACAACTGGACTAGGGTTGCCGTTTTTGCAGGCGGAAACCGCGGTCATTATCGGAC
AGATTTTGATGCTTTTGTTGGGGTGGATCGAGGCTCGCTCTGGGTCTTGGAAGAAGACTTACCTCTTGCTCTAGCAGTCG
GAGATTTTGATTCTGTGACGGAAGAAGAGCGACAGGTGATTCAAAAACGTGCCCAGTATTTTGTCCAAGCACGACCAGAA
AAGGATGATACAGATCTGGAATTGGCTCTCTTAACCATCTTTGAACAAAATCCTCAGGCTCAGGTCACTATTTTCGGTGC
CTTGGGTGGCCGTATTGACCATATGTTGGCCAATGTCTTTCTACCTAGCAATCCTAAGTTGGCACCCTATATGCGTCAAA
TAGAAATTGAGGATGGGCAAAATTTGATTGCCTATTGTTCAGAAGGGACCAGTCAGCTAGAACCTCGTTCAGACTACGAC
TATCTAGCCTTTATGCCAGTTCGGGATAGCCAGCTGACTATTCTTGGAGCTAAGTATGAGTTGACAGAGGAGAATTTTTT
CTTTAAAAAAGTGTACGCTTCTAACGAATATATAGATAGGGAAGTGTCGGTAACTTGCCCAGATGGTTATGTGGTCGTAC
TGCATAGCAAGGACAGGAGGTAG

Upstream 100 bases:

>100_bases
GAAGTGGATGGTGGGATTGATGACCAAACTATTGCTCAAGCCAAAGAAGCTGGTGCGACTGTTTTTGTAGCAGGTTCCTA
TGTCTTTAAGGGAGAAGTCA

Downstream 100 bases:

>100_bases
GATGGAAAGTTTACTTATTCTATTATTAATTGCCAACCTAGCTGGTCTCTTTCTGATTTGGCAAAGGCAGGATAGGCAGG
AGAAACACTTAAGTAAGAGC

Product: hypothetical protein

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPE
KDDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR

Sequences:

>Translated_220_residues
MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPE
KDDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR
>Mature_219_residues
SEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEK
DDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYDY
LAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 25140; Mature: 25009

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVT
CCCEEECCCCCEEEEEEECCCCCCCCCCCHHEEECCCCEEEEEECCCCEEEEECCCCCCC
EEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVF
HHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHEE
LPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYDYLAFMPVRDSQLTILGAKYE
CCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCEEEEEEEEEE
LTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR
ECCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCC
>Mature Secondary Structure 
SEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVT
CCEEECCCCCEEEEEEECCCCCCCCCCCHHEEECCCCEEEEEECCCCEEEEECCCCCCC
EEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVF
HHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHEE
LPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYDYLAFMPVRDSQLTILGAKYE
CCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCEEEEEEEEEE
LTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR
ECCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]