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The map label for this gene is tag [H]

Identifier: 116516468

GI number: 116516468

Start: 174766

End: 175329

Strand: Direct

Name: tag [H]

Synonym: SPD_0171

Alternate gene names: 116516468

Gene position: 174766-175329 (Clockwise)

Preceding gene: 116515482

Following gene: 116515703

Centisome position: 8.54

GC content: 46.63

Gene sequence:

>564_bases
ATGACAAAACGTTGTTCGTGGGTCAAGATGACCAACCCACTCTACATCGCCTATCATGATGAGGAGTGGGGCCAGCCCCT
CCATGATGACCAAGCGTTGTTTGAGTTGTTGTGTATGGAAACCTATCAGGCAGGCCTGTCTTGGGAAACGGTACTCAACA
AACGCCAAGCTTTCCGAGAAGCCTTTCATAGCTATCAAATTCACTCAGTCGCAGAGATGACTGACACTGAATTGGAAGCC
ATGCTGGAGAATCCAGCTATCATTCGAAATAGAGCCAAGATTTTTGCTACACGCGCTAACGCCCAAGCCTTTCTACAGTT
ACAGGCAGAGTACGGCTCTTTTGATGCCTATCTTTGGTCTTTTGTTGAGGGGAAAACTGTCGTTAACGATGTTCCTGATT
ATCGCCAAGCCCCAGCTAAAACACCCTTATCTGAGAAATTAGCCAAAGATCTCAAAAAACGAGGCTTCAAGTTCACAGGC
CCAGTCGCCGTATTGTCTTTTCTACAGGCTGCAGGGCTAGTTGATGACCACGAGAATGATTGTGAGTGGAAAGGTCTTAA
ATGA

Upstream 100 bases:

>100_bases
AGAGCTCAAGAAAATCAAGAAATTCTTTGAAGGAACGACAGATACAGCTGAGAACTATATCAAGTCGGCCCTTAAAATGT
TGGTCAAATAGGAGCAGAGA

Downstream 100 bases:

>100_bases
TGTCTAACAAAAATAAGGGAATTCTGATTTTTGCGATTCTCTATACAGTCCTCTTTGTGTTTGATGGCGTTAAATTGCTG
GCTTCTTTAATGCCATCTGC

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase; TAG [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQAGLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEA
MLENPAIIRNRAKIFATRANAQAFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
PVAVLSFLQAAGLVDDHENDCEWKGLK

Sequences:

>Translated_187_residues
MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQAGLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEA
MLENPAIIRNRAKIFATRANAQAFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
PVAVLSFLQAAGLVDDHENDCEWKGLK
>Mature_186_residues
TKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQAGLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAM
LENPAIIRNRAKIFATRANAQAFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTGP
VAVLSFLQAAGLVDDHENDCEWKGLK

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=179, Percent_Identity=45.8100558659218, Blast_Score=170, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 21457; Mature: 21325

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQAGLSWETVLNKRQAFRE
CCCCCCEEEECCCEEEEEECHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQAFLQLQAEYGSFDAYLWS
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCEEEEEEHHCCCCHHHHHHH
FVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTGPVAVLSFLQAAGLVDDHEND
HHCCCHHHHCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCC
CEWKGLK
CCCCCCC
>Mature Secondary Structure 
TKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQAGLSWETVLNKRQAFRE
CCCCCEEEECCCEEEEEECHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQAFLQLQAEYGSFDAYLWS
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCEEEEEEHHCCCCHHHHHHH
FVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTGPVAVLSFLQAAGLVDDHEND
HHCCCHHHHCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCC
CEWKGLK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]