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The map label for this gene is ruvA

Identifier: 116515482

GI number: 116515482

Start: 174163

End: 174756

Strand: Direct

Name: ruvA

Synonym: SPD_0170

Alternate gene names: 116515482

Gene position: 174163-174756 (Clockwise)

Preceding gene: 116516396

Following gene: 116516468

Centisome position: 8.51

GC content: 43.43

Gene sequence:

>594_bases
ATGTACGCATATTTAAAAGGAATCATTACCAAAATTACTGCCAAATACATTGTTCTTGAAACCAATGGTATTGGTTATAT
CCTGCATGTGGCCAATCCTTATGCCTATTCAGGTCAGGTTAATCAGGAGGCTCAGATTTATGTGCATCAGGTTGTGCGTG
AGGACGCCCATTTGCTTTATGGATTTCGCTCAGAGGATGAGAAAAAGCTCTTTCTTAGTCTAATTTCGGTCTCTGGGATT
GGTCCTGTATCAGCTCTTGCTATTATCGCTGCTGATGACAATGCTGGCTTGGTTCAAGCCATTGAAACCAAGAACATTAC
CTACTTGACCAAGTTCCCTAAAATTGGCAAGAAAACAGCCCAGCAGATGGTGCTGGACTTGGAAGGCAAGGTAGTAGTTG
CAGGAGATGGCCTTCCTGCCAAGGTCGCAGTGCAAGCAAGTGCTGAAAACCAAGAATTGGAAGAAGCTATGGAAGCCATG
TTGGCTCTGGGCTACAAGGCAACAGAGCTCAAGAAAATCAAGAAATTCTTTGAAGGAACGACAGATACAGCTGAGAACTA
TATCAAGTCGGCCCTTAAAATGTTGGTCAAATAG

Upstream 100 bases:

>100_bases
GATTGTTTTTGATATACTGTATTTGTGATAATCGATTCTCGCTCCTCGGATAAAGAAAATATGATATACTAGATAAACGA
AATAAGAGAGAAGGAACACT

Downstream 100 bases:

>100_bases
GAGCAGAGAATGACAAAACGTTGTTCGTGGGTCAAGATGACCAACCCACTCTACATCGCCTATCATGATGAGGAGTGGGG
CCAGCCCCTCCATGATGACC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEAQIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGI
GPVSALAIIAADDNAGLVQAIETKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK

Sequences:

>Translated_197_residues
MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEAQIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGI
GPVSALAIIAADDNAGLVQAIETKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK
>Mature_197_residues
MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEAQIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGI
GPVSALAIIAADDNAGLVQAIETKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=200, Percent_Identity=35, Blast_Score=114, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_STRP2 (Q04MQ9)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_815689.1
- ProteinModelPortal:   Q04MQ9
- SMR:   Q04MQ9
- STRING:   Q04MQ9
- EnsemblBacteria:   EBSTRT00000019951
- GeneID:   4442652
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0170
- eggNOG:   COG0632
- GeneTree:   EBGT00050000028402
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   PRK00116
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 21548; Mature: 21548

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEAQIYVHQVVREDAHLLY
CHHHHHHHHHHHHEEEEEEEECCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHCHHEEE
GFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIETKNITYLTKFPKIGKKTA
EECCCCHHHHHHHHHHHCCCCCHHEEEEEEECCCCCEEEEEECCCEEEEECCCCHHHHHH
QQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAMLALGYKATELKKIKKFFEGT
HHHHHCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
TDTAENYIKSALKMLVK
CHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEAQIYVHQVVREDAHLLY
CHHHHHHHHHHHHEEEEEEEECCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHCHHEEE
GFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIETKNITYLTKFPKIGKKTA
EECCCCHHHHHHHHHHHCCCCCHHEEEEEEECCCCCEEEEEECCCEEEEECCCCHHHHHH
QQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAMLALGYKATELKKIKKFFEGT
HHHHHCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
TDTAENYIKSALKMLVK
CHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA