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The map label for this gene is ilvB [H]

Identifier: 116516357

GI number: 116516357

Start: 404886

End: 406586

Strand: Direct

Name: ilvB [H]

Synonym: SPD_0404

Alternate gene names: 116516357

Gene position: 404886-406586 (Clockwise)

Preceding gene: 116516992

Following gene: 116515671

Centisome position: 19.79

GC content: 44.21

Gene sequence:

>1701_bases
ATGGAGAAAATCAGTTTAGAATCTCCTAAGACGGGGTCGGACCTAGTTTTGGAAACACTTCGTGATTTAGGAGTTGATAC
CATCTTTGGTTATCCTGGTGGTGCGGTTTTGCCTTTTTATGATGCGATATATAATTTTAAAGGCATTCGCCACATTCTAG
GGCGCCATGAGCAAGGTTGTTTGCATGAAGCTGAAGGCTATGCCAAATCAACTGGAAAGTTGGGTGTTGCCGTCGTCACT
AGTGGACCAGGAGCAACAAATGCCATTACAGGGATTGCGGATGCTATGAGCGATAGCGTTCCCCTTTTGGTCTTTACAGG
TCAGGTGGCGCGAGCAGGGATTGGGAAGGATGCCTTTCAGGAGGCAGACATCGTAGGAATTACCATGCCAATCACTAAGT
ACAATTACCAAGTTCGTGAGACAGCTGATATTCCGCGTATCATTACGGAAGCTGTCCATATCGCAACTACAGGCCGTCCA
GGGCCAGTTGTAATTGACCTACCAAAAGACATATCTGCTTTAGAAACAGACTTCATTTATTCATCAGAAGTGAATTTACC
AAGTTATCAGCCGACTCTTGAGCCGAATGATATGCAAATCAAGAAAATCTTGAAGCAATTGTCCAAGGCTAAAAAGCCAG
TCTTGTTAGCTGGTGGTGGAATTAGTTATGCTGAGGCTGCTACGGAACTAAATGAATTTGCAGAACGCTATCAAATTCCA
GTGGTAACCAGTCTTTTGGGACAAGGAACGATTGCAACGAGTCACCCACTCTTTCTTGGAATGGGAGGCATGCACGGGTC
ATTCGCAGCAAATATTGCCATGACGGAAGCGGACTTTATGATTAGTATTGGTTCTCGTTTCGATGACCGTTTGACGGGGA
ATCCTAAGACTTTCGCTAAGAATGCTAAGGTTGCCCACATTGATATTGACCCAGCTGAGATTGGCAAGATTATCAGTGCA
GACATTCCTGTAGTTGGAGATGCTAAGAAGGCCTTGCAAATGTTGCTAGCAGAACCAACAGTTCACAACAATACTGAAAA
GTGGATTGAGAAAGTCACTAAAGACAAGAATCGTGTTCGTTCGTATGACAAGAAAGAGCGTGTGGTTCAACCGCAAGCAG
TTATTGAACGAATTGGTGAATTGACGAATGGAGATGCCATTGTGGTAACAGACGTTGGTCAACACCAAATGTGGACAGCT
CAGTATTATCCCTACCAAAATGAACGTCAGTTAGTGACTTCAGATGGTTTGGGAACAATGGGCTTTGGAATTCCAGCAGC
AATCGGTGCTAAAATTGCTAACCCAGATAAGGAAGTAGTCTTGTTTGTTGGGGATGGTGGTTTCCAAATGACCAACCAGG
AGTTGGCTATTTTGAATATTTACAAGGTGCCAATCAAGGTGGTTATGCTGAACAATCATTCACTCGGAATGGTTCGCCAG
TGGCAGGAATCCTTCTATGAAGGCAGAACATCAGAGTCGGTCTTTGATACCCTTCCTGATTTCCAATTGATGGCGCAGGC
TTATGGTATTAAAAACTATAAGTTTGACAATCCTGAGACCTTGGCTCAAGACCTTGAAGTCATCACTGAGGATGTTCCTA
TGCTAATTGAGGTAGATATTTCTCGTAAGGAACAGGTGTTACCAATGGTACCGGCTGGTAAGAGTAATCATGAGATGTTG
GGGGTGCAGTTCCATGCGTAG

Upstream 100 bases:

>100_bases
AAAATAGAAAATTCAGAAAATTTCTTCTTTTATCTTGAAAAATTTTGAAAAAATGGTATGATAGTAACAAGTTATTTTTA
AGAGGAAAGAAAGGGGAATA

Downstream 100 bases:

>100_bases
AATGTTAACAGCAAAACTACAAAATCGTTCAGGAGTCCTCAATCGCTTTACAGGTGTCCTATCTCGTCGTCAGGTTAATA
TTGAAAGCATCTCTGTTGGA

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIYNFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVT
SGPGATNAITGIADAMSDSVPLLVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
GPVVIDLPKDISALETDFIYSSEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAGGGISYAEAATELNEFAERYQIP
VVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTEADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISA
DIPVVGDAKKALQMLLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDVGQHQMWTA
QYYPYQNERQLVTSDGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQMTNQELAILNIYKVPIKVVMLNNHSLGMVRQ
WQESFYEGRTSESVFDTLPDFQLMAQAYGIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEML
GVQFHA

Sequences:

>Translated_566_residues
MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIYNFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVT
SGPGATNAITGIADAMSDSVPLLVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
GPVVIDLPKDISALETDFIYSSEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAGGGISYAEAATELNEFAERYQIP
VVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTEADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISA
DIPVVGDAKKALQMLLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDVGQHQMWTA
QYYPYQNERQLVTSDGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQMTNQELAILNIYKVPIKVVMLNNHSLGMVRQ
WQESFYEGRTSESVFDTLPDFQLMAQAYGIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEML
GVQFHA
>Mature_566_residues
MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIYNFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVT
SGPGATNAITGIADAMSDSVPLLVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
GPVVIDLPKDISALETDFIYSSEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAGGGISYAEAATELNEFAERYQIP
VVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTEADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISA
DIPVVGDAKKALQMLLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDVGQHQMWTA
QYYPYQNERQLVTSDGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQMTNQELAILNIYKVPIKVVMLNNHSLGMVRQ
WQESFYEGRTSESVFDTLPDFQLMAQAYGIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEML
GVQFHA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=569, Percent_Identity=25.6590509666081, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI21361361, Length=519, Percent_Identity=24.8554913294798, Blast_Score=150, Evalue=4e-36,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=46.085409252669, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI1790104, Length=556, Percent_Identity=40.8273381294964, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1786717, Length=552, Percent_Identity=35.3260869565217, Blast_Score=339, Evalue=4e-94,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=28.021978021978, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788716, Length=480, Percent_Identity=27.2916666666667, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17531299, Length=487, Percent_Identity=29.5687885010267, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=487, Percent_Identity=29.5687885010267, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=531, Percent_Identity=24.6704331450094, Blast_Score=134, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=575, Percent_Identity=42.2608695652174, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320816, Length=475, Percent_Identity=24, Blast_Score=120, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6320123, Length=540, Percent_Identity=23.1481481481481, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=561, Percent_Identity=24.0641711229947, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323073, Length=476, Percent_Identity=24.1596638655462, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=560, Percent_Identity=24.1071428571429, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19922626, Length=568, Percent_Identity=26.7605633802817, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61740; Mature: 61740

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIYNFKGIRHILGRHEQGC
CCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
LHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPLLVFTGQVARAGIGKDAFQ
HHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHC
EADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRPGPVVIDLPKDISALETDFIY
CCCEEEEEEEEEECCEEEECCCCHHHHHHHHHHEEECCCCCCEEEECCCCCHHHHHCEEE
SSEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAGGGISYAEAATELNEFAERYQIP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
VVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTEADFMISIGSRFDDRLTGNPKTFAK
HHHHHHCCCCEEECCCEEEEECCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCHHHHC
NAKVAHIDIDPAEIGKIISADIPVVGDAKKALQMLLAEPTVHNNTEKWIEKVTKDKNRVR
CCEEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SYDKKERVVQPQAVIERIGELTNGDAIVVTDVGQHQMWTAQYYPYQNERQLVTSDGLGTM
CCCHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEECCCCCCC
GFGIPAAIGAKIANPDKEVVLFVGDGGFQMTNQELAILNIYKVPIKVVMLNNHSLGMVRQ
CCCCHHHHCCCCCCCCCEEEEEEECCCCEECCCCEEEEEEEECCEEEEEECCCCCHHHHH
WQESFYEGRTSESVFDTLPDFQLMAQAYGIKNYKFDNPETLAQDLEVITEDVPMLIEVDI
HHHHHHCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
SRKEQVLPMVPAGKSNHEMLGVQFHA
CCCCCCCEECCCCCCCCEEEEEEECC
>Mature Secondary Structure
MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIYNFKGIRHILGRHEQGC
CCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
LHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPLLVFTGQVARAGIGKDAFQ
HHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHC
EADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRPGPVVIDLPKDISALETDFIY
CCCEEEEEEEEEECCEEEECCCCHHHHHHHHHHEEECCCCCCEEEECCCCCHHHHHCEEE
SSEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAGGGISYAEAATELNEFAERYQIP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
VVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTEADFMISIGSRFDDRLTGNPKTFAK
HHHHHHCCCCEEECCCEEEEECCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCHHHHC
NAKVAHIDIDPAEIGKIISADIPVVGDAKKALQMLLAEPTVHNNTEKWIEKVTKDKNRVR
CCEEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SYDKKERVVQPQAVIERIGELTNGDAIVVTDVGQHQMWTAQYYPYQNERQLVTSDGLGTM
CCCHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEECCCCCCC
GFGIPAAIGAKIANPDKEVVLFVGDGGFQMTNQELAILNIYKVPIKVVMLNNHSLGMVRQ
CCCCHHHHCCCCCCCCCEEEEEEECCCCEECCCCEEEEEEEECCEEEEEECCCCCHHHHH
WQESFYEGRTSESVFDTLPDFQLMAQAYGIKNYKFDNPETLAQDLEVITEDVPMLIEVDI
HHHHHHCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
SRKEQVLPMVPAGKSNHEMLGVQFHA
CCCCCCCEECCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400210; 11337471 [H]