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The map label for this gene is pyrF

Identifier: 116516349

GI number: 116516349

Start: 631944

End: 632645

Strand: Direct

Name: pyrF

Synonym: SPD_0608

Alternate gene names: 116516349

Gene position: 631944-632645 (Clockwise)

Preceding gene: 116515841

Following gene: 116515534

Centisome position: 30.89

GC content: 45.87

Gene sequence:

>702_bases
ATGCGAGAACATCGTCCAATCATTGCTCTTGATTTTCCTAGTTTTGAGGCGGTCAAGGAATTTTTAGCTCTTTTCCCAGC
AGAAGAAAGCCTTTATCTCAAGGTAGGGATGGAGCTTTATTACGCAGCGGGGCCTGAGATTGTGTCCTACTTAAAAGGTT
TGGGTCATAGTGTCTTTTTGGATCTCAAACTTCATGACATTCCTAATACAGTCAAGTCAGCCATGAAGATCCTATCTCAG
CTTGGTGTGGATATGACCAATGTTCATGCGGCTGGTGGTGTAGAGATGATGAAGGCTGCGCGTGAAGGTCTTGGAAGTCA
AGCTAAATTGATCGCTGTAACTCAGCTCACATCAACGTCAGAAGCTCAGATGCAGGAGTTTCAAAATATCCAAACCAGTC
TGCAAGAGTCTGTGATTCACTATGCCAAGAAGACAGCTGAAGCTGGCTTGGATGGTGTTGTTTGCTCGGCTCAGGAAGTA
CAAGTCATCAAGCAGGCTACCAATCCAGATTTTATCTGTCTGACACCAGGGATTCGTCCAGCTGGTGTTGCAGTTGGAGA
TCAAAAACGAGTCATGACACCTGCTGATGCCTATCAAATCGGCAGTGACTATATCGTAGTGGGACGTCCCATTACCCAAG
CTGAGGAGCCTGTTGCAGCTTATCATGCCATCAAGGATGAATGGACACAGGACTGGAATTAA

Upstream 100 bases:

>100_bases
GACTACCCATTTTTCGTGGAACCTTGCTGTTGGCAGGTTCCTTTTTTCGTGGCTTCTGTTGGCCAGACTCTCTCACTAGT
AAAGGTAAAAGGAGAAACCT

Downstream 100 bases:

>100_bases
AGAACTAGATTAGAAAAATAAAAGGAGAATACCATGACACTTGCTAAAGATATCGCTAGCCACCTCTTGAAAATCCAAGC
CGTTTACCTCAAACCAGAGG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAGPEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQ
LGVDMTNVHAAGGVEMMKAAREGLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEEPVAAYHAIKDEWTQDWN

Sequences:

>Translated_233_residues
MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAGPEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQ
LGVDMTNVHAAGGVEMMKAAREGLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEEPVAAYHAIKDEWTQDWN
>Mature_233_residues
MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAGPEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQ
LGVDMTNVHAAGGVEMMKAAREGLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEEPVAAYHAIKDEWTQDWN

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=223, Percent_Identity=43.4977578475336, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_STRP2 (Q04LJ3)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816105.1
- ProteinModelPortal:   Q04LJ3
- SMR:   Q04LJ3
- STRING:   Q04LJ3
- EnsemblBacteria:   EBSTRT00000018841
- GeneID:   4442951
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0608
- eggNOG:   COG0284
- GeneTree:   EBGT00050000027603
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25437; Mature: 25437

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 63-63 BINDING 11-11 BINDING 34-34 BINDING 117-117 BINDING 179-179 BINDING 188-188 BINDING 208-208 BINDING 209-209

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAGPEIVSYLKGLGHSVFL
CCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEECEEEEECCCHHHHHHHHHCCCEEEE
DLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAAREGLGSQAKLIAVTQLTSTS
EEEECCCCHHHHHHHHHHHHHCCCHHHEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCC
EAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEVQVIKQATNPDFICLTPGIRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCEEEECCCCCC
AGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEEPVAAYHAIKDEWTQDWN
CCEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAGPEIVSYLKGLGHSVFL
CCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEECEEEEECCCHHHHHHHHHCCCEEEE
DLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAAREGLGSQAKLIAVTQLTSTS
EEEECCCCHHHHHHHHHHHHHCCCHHHEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCC
EAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEVQVIKQATNPDFICLTPGIRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCEEEECCCCCC
AGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEEPVAAYHAIKDEWTQDWN
CCEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA