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The map label for this gene is pyrE

Identifier: 116515534

GI number: 116515534

Start: 632679

End: 633311

Strand: Direct

Name: pyrE

Synonym: SPD_0609

Alternate gene names: 116515534

Gene position: 632679-633311 (Clockwise)

Preceding gene: 116516349

Following gene: 116516064

Centisome position: 30.92

GC content: 44.08

Gene sequence:

>633_bases
ATGACACTTGCTAAAGATATCGCTAGCCACCTCTTGAAAATCCAAGCCGTTTACCTCAAACCAGAGGAACCCTTCACTTG
GGCATCTGGTATCAAGTCACCGATTTACACTGATAATCGTGTGACACTAGCCTATCCAGAAACTCGTACCCTAATTGAAA
ATGGTTTTGTGGAAGCTATCAAAGAAGCCTTTCCTGAAGTAGAAGTGATTGCAGGAACTGCAACAGCAGGGATTCCACAC
GGAGCCATTATTGCTGATAAGATGGACTTGCCTTTTGCCTACATCCGTAGCAAACCAAAAGACCACGGAGCTGGTAATCA
AATCGAAGGTCGCGTAGCTCAAGGTCAAAAAATGGTAGTGGTTGAAGACCTTATTTCAACGGGTGGTTCAGTTCTTGAAG
CTGTAGCAGCAGCCAAGCGAGAAGGAGCAGATGTGCTTGGAGTTGTAGCGATTTTCAGCTACCAATTGCCAAAAGCAGAT
AAGAACTTTGCAGATGCTGGTGTTAAACTTGTGACGCTTTCAAACTATAGCGATCTTATCCATCTAGCCCAAGAAGAAGG
TTACATCACGCCAGAGGGCCTTTATCTTCTAAAACGCTTTAAAGAAGACCAAGAAAATTGGCAAGAAGGTTAG

Upstream 100 bases:

>100_bases
CCAAGCTGAGGAGCCTGTTGCAGCTTATCATGCCATCAAGGATGAATGGACACAGGACTGGAATTAAAGAACTAGATTAG
AAAAATAAAAGGAGAATACC

Downstream 100 bases:

>100_bases
GTCAGTAAGATAAAGAGAGACGAGGCTACCGAGCCTCTTTTACCATTTTGTTTAAAATATGACAATATTTTAAACAAAAT
AATTGACAACGCTTTCTTTT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLAYPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPH
GAIIADKMDLPFAYIRSKPKDHGAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
KNFADAGVKLVTLSNYSDLIHLAQEEGYITPEGLYLLKRFKEDQENWQEG

Sequences:

>Translated_210_residues
MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLAYPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPH
GAIIADKMDLPFAYIRSKPKDHGAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
KNFADAGVKLVTLSNYSDLIHLAQEEGYITPEGLYLLKRFKEDQENWQEG
>Mature_209_residues
TLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLAYPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHG
AIIADKMDLPFAYIRSKPKDHGAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKADK
NFADAGVKLVTLSNYSDLIHLAQEEGYITPEGLYLLKRFKEDQENWQEG

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=182, Percent_Identity=25.2747252747253, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1790073, Length=191, Percent_Identity=27.2251308900524, Blast_Score=62, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17933654, Length=190, Percent_Identity=27.3684210526316, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_STRP2 (Q04LJ2)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816106.1
- ProteinModelPortal:   Q04LJ2
- SMR:   Q04LJ2
- STRING:   Q04LJ2
- EnsemblBacteria:   EBSTRT00000019105
- GeneID:   4442899
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0609
- eggNOG:   COG0461
- GeneTree:   EBGT00050000027746
- HOGENOM:   HBG404341
- OMA:   LPMTYVR
- ProtClustDB:   PRK00455
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 22875; Mature: 22744

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 96-96 BINDING 100-100 BINDING 102-102 BINDING 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLAYPETRTLIENGFVEAI
CCHHHHHHHHHHHHEEEEECCCCCCHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHH
KEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDHGAGNQIEGRVAQGQKMVV
HHHCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHCCCCCCCCCCCCCCHHCCCCEEEE
VEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKADKNFADAGVKLVTLSNYSDLI
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHCCEEEEEECCHHHHH
HLAQEEGYITPEGLYLLKRFKEDQENWQEG
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLAYPETRTLIENGFVEAI
CHHHHHHHHHHHHEEEEECCCCCCHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHH
KEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDHGAGNQIEGRVAQGQKMVV
HHHCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHCCCCCCCCCCCCCCHHCCCCEEEE
VEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKADKNFADAGVKLVTLSNYSDLI
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHCCEEEEEECCHHHHH
HLAQEEGYITPEGLYLLKRFKEDQENWQEG
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA