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The map label for this gene is glpF [H]

Identifier: 116516308

GI number: 116516308

Start: 1987074

End: 1987778

Strand: Reverse

Name: glpF [H]

Synonym: SPD_2011

Alternate gene names: 116516308

Gene position: 1987778-1987074 (Counterclockwise)

Preceding gene: 116516239

Following gene: 116515697

Centisome position: 97.15

GC content: 46.1

Gene sequence:

>705_bases
ATGATGAATGAATTATTTGGAGAATTTCTAGGGACTTTAATCCTGATTCTTCTAGGAAATGGTGTTGTTGCAGGTGTGGT
TCTTCCTAAAACCAAGAGCAATAGCTCAGGTTGGATTGTGATTACTATGGGTTGGGGGATTGCAGTTGCGGTTGCAGTCT
TTGTATCTGGCAAGCTCAGTCCAGCTCATTTAAACCCAGCTGTGACCATCGGTGTGGCCTTAAAAGGTGGTTTGCCTTGG
GCTTCCGTTTTGCCTTATATCTTAGCCCAGTTCGCAGGGGCCATGCTGGGTCAGATTTTGGTTTGGTTGCAATTCAAACC
TCACTATGAGGCAGAAGAAAATGCAGGCAATATCCTGGCAACCTTCAGTACTGGACCAGCCATCAAGGATACTGTATCAA
ACTTGATTAGCGAAATCCTTGGAACCTTTGTTTTGGTGTTGACAATCTTTGCTTTGGGTCTTTACGATTTTCAGGCAGGT
ATCGGAACCTTTGCAGTGGGAACTTTGATTGTCGGTATCGGTCTATCACTAGGTGGGACAACAGGTTATGCCTTGAACCC
AGCTCGTGACCTTGGACCTCGTATCATGCACAGCATCTTGCCAATTCCAAACAAGGGAGACGGAGACTGGTCTTACGCTT
GGATTCCTGTTGTAGGCCCTGTTATCGGAGCAGCCTTGGCCGTGCTTGTATTCTCACTTTTCTAA

Upstream 100 bases:

>100_bases
TAACAACGATTTAGCAGAATTAAAAAATTAAGAAAAAATAAAAGAGGTGGAGGGCAGCATTCCTTGTCGCCCGTCCCTTC
TTTTTAATGGAGACAGAAAG

Downstream 100 bases:

>100_bases
TCTAGAAAACAATTATGTTGATAGAGCTTGGGCAAGAGCCCAATTTCAGCAAAAAATGAAGTAAATCTTCTCATAATAAA
ACGCATCATATCAAGCACGA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMGWGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPW
ASVLPYILAQFAGAMLGQILVWLQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVVGPVIGAALAVLVFSLF

Sequences:

>Translated_234_residues
MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMGWGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPW
ASVLPYILAQFAGAMLGQILVWLQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVVGPVIGAALAVLVFSLF
>Mature_234_residues
MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMGWGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPW
ASVLPYILAQFAGAMLGQILVWLQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVVGPVIGAALAVLVFSLF

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=256, Percent_Identity=34.375, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI4826645, Length=250, Percent_Identity=32.4, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI22538420, Length=255, Percent_Identity=32.5490196078431, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI4502187, Length=251, Percent_Identity=31.8725099601594, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI4755125, Length=228, Percent_Identity=29.8245614035088, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI4502181, Length=228, Percent_Identity=29.8245614035088, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI37694062, Length=233, Percent_Identity=27.8969957081545, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI6912506, Length=243, Percent_Identity=28.8065843621399, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1790362, Length=251, Percent_Identity=32.2709163346614, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71994009, Length=250, Percent_Identity=32, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17544068, Length=258, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531429, Length=241, Percent_Identity=30.2904564315353, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17531431, Length=241, Percent_Identity=30.2904564315353, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17558372, Length=238, Percent_Identity=30.2521008403361, Blast_Score=94, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71992966, Length=251, Percent_Identity=27.8884462151394, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17533613, Length=237, Percent_Identity=29.1139240506329, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI32564052, Length=237, Percent_Identity=29.1139240506329, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71992961, Length=239, Percent_Identity=26.3598326359833, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71993722, Length=235, Percent_Identity=28.5106382978723, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=210, Percent_Identity=35.7142857142857, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322985, Length=284, Percent_Identity=26.056338028169, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24652747, Length=232, Percent_Identity=30.6034482758621, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI45551084, Length=232, Percent_Identity=30.6034482758621, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24762342, Length=239, Percent_Identity=27.6150627615063, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24345; Mature: 24345

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMGWGIAVAVAVFVSGKLS
CCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHCCCCC
PAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVWLQFKPHYEAEENAGNILA
CCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
TFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAGIGTFAVGTLIVGIGLSLGGT
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
TGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVVGPVIGAALAVLVFSLF
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMGWGIAVAVAVFVSGKLS
CCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHCCCCC
PAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVWLQFKPHYEAEENAGNILA
CCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
TFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAGIGTFAVGTLIVGIGLSLGGT
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
TGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVVGPVIGAALAVLVFSLF
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7565084; 11463916 [H]