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The map label for this gene is pmrA

Identifier: 116516183

GI number: 116516183

Start: 873741

End: 874940

Strand: Direct

Name: pmrA

Synonym: SPD_0860

Alternate gene names: 116516183

Gene position: 873741-874940 (Clockwise)

Preceding gene: 116516997

Following gene: 116516845

Centisome position: 42.7

GC content: 43.17

Gene sequence:

>1200_bases
ATGACAGAGATTAACTGGAAGGATAATCTGCGTATTGCCTGGTTTGGTAATTTTCTGACAGGAGCCAGTATTTCTTTGGT
TGTACCTTTTATGCCCATCTTCGTGGAAAATCTAGGTGTAGGGAGTCAGCAAGTCGCTTTTTATGCAGGCTTAGCAATTT
CTGTCTCTGCTATTTCCGCGGCGCTCTTTTCTCCTATTTGGGGTATTCTTGCTGACAAATACGGCCGAAAACCCATGATG
ATTCGGGCAGGTCTTGCTATGACTATCACTATGGGAGGCTTGGCCTTTGTCCCAAATATCTATTGGTTAATCTTTCTTCG
TTTACTAAACGGTGTATTTGCAGGTTTTGTTCCTAATGCAACGGCACTGATAGCCAGTCAGGTTCCAAAGGAGAAATCAG
GCTCTGCCTTAGGTACTTTGTCTACAGGCGTAGTTGCAGGTACTCTAACCGGTCCCTTTATTGGTGGCTTTATCGCAGAA
TTATTTGGCATTCGTACAGTTTTCTTACTGGTTGGTAGTTTTCTATTTTTAGCTGCTATTTTGACTATTTGCTTTATCAA
GGAAGATTTTCAACCAGTAGCCAAGGAAAAGGCTATTCCAACAAAGGAATTATTTACCTCGGTTAAATATCCCTATCTTT
TGCTCAATCTCTTTTTAACCAGTTTTGTCATCCAATTTTCAGCTCAATCGATCGGCCCTATTTTGGCTCTTTATGTACGC
GACTTAGGGCAGACAGAGAATCTTCTTTTTGTCTCTGGTTTGATTGTGTCCAGTATGGGCTTTTCCAGTATGATGAGTGC
AGGAGTCATGGGCAAGCTAGGTGACAAGGTGGGCAATCATCGTCTCTTGGTTGTCGCCCAGTTTTATTCAGTCATCATCT
ATCTCCTCTGTGCCAATGCCTCTAGCCCCCTTCAACTAGGACTCTATCGTTTCCTCTTTGGCTTGGGAACCGGTGCCTTG
ATTCCAGGGGTTAATGCCCTACTCAGCAAAATGACTCCCAAAGCCGGCATTTCGAGGGTCTTTGCCTTCAATCAGGTATT
CTTTTATCTGGGAGGTGTTGTTGGTCCCATGGCAGGTTCTGCAGTAGCAGGTCAATTTGGCTACCATGCTGTCTTTTATG
CGACAAGCCTTTGTGTTGCCTTTAGTTGTCTCTTTAACCTGATTCAATTTCGAACATTATTAAAAGTAAAGGAAATCTAG

Upstream 100 bases:

>100_bases
GAAAAAAAGAAAGATTTGGCCAGCCAGGTTCTTGATAATAATGGCAATCAGAACCAGCTTCTTAATCAAGTGCATATCCT
TCTTGAGGGAGGTAGGCAAG

Downstream 100 bases:

>100_bases
TGCGAGTAAAAATTAATCTCAAATGCTCCTCTTGTGACAGTATCAATTACCTAACCAGTAAAAATTCAAAAACCCATCCA
GACAAGATTGAGGTTTTAAA

Product: multi-drug resistance efflux pump

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQVAFYAGLAISVSAISAALFSPIWGILADKYGRKPMM
IRAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLFLTSFVIQFSAQSIGPILALYVR
DLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGAL
IPGVNALLSKMTPKAGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRTLLKVKEI

Sequences:

>Translated_399_residues
MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQVAFYAGLAISVSAISAALFSPIWGILADKYGRKPMM
IRAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLFLTSFVIQFSAQSIGPILALYVR
DLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGAL
IPGVNALLSKMTPKAGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRTLLKVKEI
>Mature_398_residues
TEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQVAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMI
RAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAEL
FGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLFLTSFVIQFSAQSIGPILALYVRD
LGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALI
PGVNALLSKMTPKAGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRTLLKVKEI

Specific function: Efflux pump for various substrates

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family

Homologues:

Organism=Escherichia coli, GI1787291, Length=392, Percent_Identity=44.8979591836735, Blast_Score=326, Evalue=1e-90,
Organism=Escherichia coli, GI87082404, Length=389, Percent_Identity=24.9357326478149, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PMRA_STRPN (P0A4K4)

Other databases:

- EMBL:   AJ007367
- EMBL:   AE005672
- PIR:   D95112
- RefSeq:   NP_345453.1
- ProteinModelPortal:   P0A4K4
- EnsemblBacteria:   EBSTRT00000026594
- GeneID:   931481
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_0972
- TIGR:   SP_0972
- GeneTree:   EBGT00050000026899
- HOGENOM:   HBG404070
- OMA:   ASYLIFM
- ProtClustDB:   CLSK625886
- BioCyc:   SPNE170187-1:SP_0972-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR001958
- PRINTS:   PR01035

Pfam domain/function: PF07690 MFS_1; PF00083 Sugar_tr; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 42841; Mature: 42709

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b4aafc0)-; HASH(0x1b4edba4)-; HASH(0x1acd61f0)-; HASH(0x1b1790e0)-; HASH(0x1b4548b0)-; HASH(0x1ac1cbe4)-; HASH(0x19cbb3f4)-; HASH(0x1b28352c)-; HASH(0x1ac91e38)-; HASH(0x1ae5d16c)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQVAFYAGLAISVSAISA
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGFVPNA
HHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
TALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAELFGIRTVFLLVGSFLFLAAI
HHHHHHHCCCCCCCCCCEEHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLFLTSFVIQFSAQSIGPILALYVR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCANA
HCCCCCCCHHHHHHHHHHCCHHHHHHHCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCC
SSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPKAGISRVFAFNQVFFYLGGVVGPMAGS
CCHHHHHHHHHHHHCCCCCHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AVAGQFGYHAVFYATSLCVAFSCLFNLIQFRTLLKVKEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
TEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQVAFYAGLAISVSAISA
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGFVPNA
HHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
TALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAELFGIRTVFLLVGSFLFLAAI
HHHHHHHCCCCCCCCCCEEHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLFLTSFVIQFSAQSIGPILALYVR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCANA
HCCCCCCCHHHHHHHHHHCCHHHHHHHCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCC
SSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPKAGISRVFAFNQVFFYLGGVVGPMAGS
CCHHHHHHHHHHHHCCCCCHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AVAGQFGYHAVFYATSLCVAFSCLFNLIQFRTLLKVKEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9869592; 11463916