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The map label for this gene is aroE

Identifier: 116516036

GI number: 116516036

Start: 1238964

End: 1239818

Strand: Reverse

Name: aroE

Synonym: SPD_1210

Alternate gene names: 116516036

Gene position: 1239818-1238964 (Counterclockwise)

Preceding gene: 116517065

Following gene: 116516860

Centisome position: 60.59

GC content: 43.63

Gene sequence:

>855_bases
ATGAAGCTTGATGGCTATACACGTTTAGCTGCAGTTGTTGCCAATCCTATTAAGCATTCTATTTCTCCCTTCATCCACAA
TAGAGCCTTTGAGGCGACAGCTACCAACGGTGCTTATGTGGCTTGGGAGATTGAAGCGAGTGACTTGGTAGAAACAGTGG
CCAATATTCGTCGCTACCAGATGTTTGGCATCAATCTGTCCATGCCCTATAAGGAGCAGGTGATTCCTTATTTGGATAAG
CTGAGCGATGAAGCGCGCTTGATTGGTGCGGTTAATACGGTTGTCAATGAGAATGGCAATTTAATTGGATATAATACAGA
TGGCAAGGGATTTTTTAAGTGCTTGCCTTCTTTTACAATTTCAGGTAAAAAGATGACCCTGCTGGGTGCAGGTGGTGCGG
CTAAATCAATCTTGGCACAGGCTATTTTGGATGGCGTCAGTCAGATTTCGGTCTTTGTTCGTTCCGTTTCTATGGAAAAA
ACAAGACCTTACCTAGACAAGTTACAGGAGCAGACAGGCTTTAAAGTGGATTTGTGTGCTTTAGAATATGTTTCTGAACT
GCAAGCAAGGATTGCCGAGTCGGATTTGCTAGTTAATGCCACCAGTGTGGGCATGGATGGCCAATCCTCTCCTGTTCCTG
AAAACATAGTCTTACCAGAAACTCTCTTAGTTGCAGATATCATATACCAACCCTTTGAAACACCGTTTTTGAAATGGGCT
AGAAGTCAGGGCAATCCAGCAGTCAATGGTCTGGGAATGTTGCTCTATCAAGCTGCAGAAGCTTTTCAACTGTGGACAGG
CAAGGAAATGCCGACAGAAGAGATTTGGCAGTCTTTAACAGAAAAATACCAATAA

Upstream 100 bases:

>100_bases
GATGTGACTGGTTCGAGTTGGTCTTTTGCCAGTCTGGATGAGGTTAGTGCCCCTGGGCAGATTTCTCTAGCTAGCATGAA
AAAAATCAGGGAGATTTTGG

Downstream 100 bases:

>100_bases
AGAAAGGGAGACTCTCCTATGAAAATCAGAATCGATATTCCTCATCATCCTTATGATATTCAGATTGAAAAAGGTTGTAT
GGCCCAGGCTGGTCAGTGGT

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEIEASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDK
LSDEARLIGAVNTVVNENGNLIGYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVLPETLLVADIIYQPFETPFLKWA
RSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIWQSLTEKYQ

Sequences:

>Translated_284_residues
MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEIEASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDK
LSDEARLIGAVNTVVNENGNLIGYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVLPETLLVADIIYQPFETPFLKWA
RSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIWQSLTEKYQ
>Mature_284_residues
MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEIEASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDK
LSDEARLIGAVNTVVNENGNLIGYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVLPETLLVADIIYQPFETPFLKWA
RSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIWQSLTEKYQ

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787983, Length=284, Percent_Identity=35.9154929577465, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1789675, Length=276, Percent_Identity=35.5072463768116, Blast_Score=138, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6320332, Length=284, Percent_Identity=26.7605633802817, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_STRP2 (Q04JX1)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816677.1
- ProteinModelPortal:   Q04JX1
- SMR:   Q04JX1
- STRING:   Q04JX1
- EnsemblBacteria:   EBSTRT00000020682
- GeneID:   4441696
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1210
- eggNOG:   COG0169
- GeneTree:   EBGT00050000027337
- HOGENOM:   HBG553408
- OMA:   NARYLAF
- ProtClustDB:   PRK00258
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 31270; Mature: 31270

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: ACT_SITE 71-71

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEIEASDLVETVANIRRYQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECHHHHHHHHHHHHHHE
MFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLIGYNTDGKGFFKCLPSFTI
EEECEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCEE
SGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEKTRPYLDKLQEQTGFKVDLCA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHH
LEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVLPETLLVADIIYQPFETPFLKWA
HHHHHHHHHHHHHHHHEEEEHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
RSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIWQSLTEKYQ
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEIEASDLVETVANIRRYQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEECHHHHHHHHHHHHHHE
MFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLIGYNTDGKGFFKCLPSFTI
EEECEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCEE
SGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEKTRPYLDKLQEQTGFKVDLCA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHH
LEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVLPETLLVADIIYQPFETPFLKWA
HHHHHHHHHHHHHHHHEEEEHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
RSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIWQSLTEKYQ
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA