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The map label for this gene is nth [H]

Identifier: 116516035

GI number: 116516035

Start: 1168572

End: 1169201

Strand: Reverse

Name: nth [H]

Synonym: SPD_1135

Alternate gene names: 116516035

Gene position: 1169201-1168572 (Counterclockwise)

Preceding gene: 116516761

Following gene: 116515708

Centisome position: 57.14

GC content: 44.6

Gene sequence:

>630_bases
ATGGTCTTGTCAAAAAAACGAGCACGAAAGGTGCTAGAAGAAATCATTGCCCTCTTCCCAGATGCCAAGCCTAGTCTTGA
TTTTACCAATCATTTTGAACTCCTGGTTGCGGTCATGTTATCAGCCCAGACGACAGATGCAGCGGTAAATAAGGCCACAC
CAGGTCTCTTTGTTGCCTTTCCAACACCCCAAGCTATGTCTGTAGCGACAGAGAGTGAGATTGCTTCACATATTTCTCGC
TTGGGATTGTATCGGAATAAAGCTAAATTCCTTAAAAAATGTGCCCAACAGTTACTAGACGATTTTGATGGTCAAGTCCC
TCAGACACGTGAAGAATTGGAGAGTTTGGCAGGTGTTGGTCGCAAGACAGCCAATGTTGTCATGAGTGTAGGATTTGGGA
TTCCAGCCTTTGCAGTGGATACTCATGTGGAGCGTATTTGCAAACACCACGATATTGTCAAAAAATCAGCGACGCCACTT
GAGGTGGAAAAGCGGGTCATGGATATCTTGCCGCCTGAGCAGTGGTTAGCTGCCCATCAGGCCATGATTTATTTTGGAAG
AGCCATTTGCCATCCAAAAAATCCAGAGTGTGACCAGTACCCACAATTATATGATTTTAGCAATTTGTAA

Upstream 100 bases:

>100_bases
AAATCATGACAGAGGAAGAATACCAAGCTCAAAAAGCAGTAAAGAAAGAAGAAAACAGTCCTTTTGCTGGCTTACAAGGA
CTATTTGACGGAGATGAATA

Downstream 100 bases:

>100_bases
GATGCTACATTGAAACTTTTTGCTTGATTTTAAGCATGCTTTGTGCTATAGTAAATGATAACTAAATATTCCTTTAAACC
TGTCCCGTGAGGCAGGCAAG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQTTDAAVNKATPGLFVAFPTPQAMSVATESEIASHISR
LGLYRNKAKFLKKCAQQLLDDFDGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL

Sequences:

>Translated_209_residues
MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQTTDAAVNKATPGLFVAFPTPQAMSVATESEIASHISR
LGLYRNKAKFLKKCAQQLLDDFDGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL
>Mature_209_residues
MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQTTDAAVNKATPGLFVAFPTPQAMSVATESEIASHISR
LGLYRNKAKFLKKCAQQLLDDFDGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=168, Percent_Identity=30.952380952381, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI115298648, Length=175, Percent_Identity=27.4285714285714, Blast_Score=71, Evalue=5e-13,
Organism=Homo sapiens, GI190358497, Length=175, Percent_Identity=27.4285714285714, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI6912520, Length=175, Percent_Identity=27.4285714285714, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI115298654, Length=175, Percent_Identity=27.4285714285714, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI115298652, Length=175, Percent_Identity=27.4285714285714, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI115298650, Length=175, Percent_Identity=27.4285714285714, Blast_Score=71, Evalue=7e-13,
Organism=Escherichia coli, GI1787920, Length=195, Percent_Identity=37.9487179487179, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1789331, Length=182, Percent_Identity=31.3186813186813, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=34.6368715083799, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=180, Percent_Identity=30.5555555555556, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319304, Length=176, Percent_Identity=27.8409090909091, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550361, Length=173, Percent_Identity=31.2138728323699, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23277; Mature: 23277

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQTTDAAVNKATPGLFVAF
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEE
PTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDFDGQVPQTREELESLAGVG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
RKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPLEVEKRVMDILPPEQWLAAHQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHH
AMIYFGRAICHPKNPECDQYPQLYDFSNL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQTTDAAVNKATPGLFVAF
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEE
PTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDFDGQVPQTREELESLAGVG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
RKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPLEVEKRVMDILPPEQWLAAHQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHH
AMIYFGRAICHPKNPECDQYPQLYDFSNL
HHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]