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The map label for this gene is ksgA

Identifier: 116515767

GI number: 116515767

Start: 1777816

End: 1778688

Strand: Reverse

Name: ksgA

Synonym: SPD_1782

Alternate gene names: 116515767

Gene position: 1778688-1777816 (Counterclockwise)

Preceding gene: 116517198

Following gene: 116516502

Centisome position: 86.93

GC content: 46.39

Gene sequence:

>873_bases
ATGAGAATTGCAGATTATAGCGTGACCAAGGCAGTGCTGGAGCGTCACGGTTTTACCTTTAAAAAGTCCTTCGGGCAAAA
TTTCCTGACGGATACCAATATCCTTCAAAAAATTGTGGATACGGCTGAAATTGATGATCAGGTCAATGTCATCGAAATCG
GGCCAGGTATTGGTGCCTTGACAGAATTTTTGGCTGAGCGTGCAGCCCAAGTCATGGCTTTTGAGATTGACCACCGTTTG
GTGCCAATTTTGGCAGATACCCTGCGTGATTTTGATAATGTGACCGTAGTTAACGAAGATATTCTCAAGGTTGATTTGGC
GCAACATATCCAAAATTTTAAAAATCCTAACCTGCCAATCAAGGTAGTGGCTAATTTGCCTTACTACATCACGACGCCTA
TTCTCATGCACTTGATTGAGAGTGGCATTCCTTTTAGTGAGTTTGTGGTCATGATGCAGAAAGAAGTAGCGGACCGCATT
TCAGCCCAGCCTAACACCAAGGCTTACGGTAGCTTGTCTATCGCCGTGCAGTATTACATGACAGCCAAGGTTGCCTTTAT
CGTGCCTCGTACGGTCTTTGTGCCAGCGCCAAATGTGGATTCAGCCATCTTGAAAATGGTGCGTCGTCCAGAGCCAGCCG
TAGCAGTAGAAGATGAGAACTTTTTCTTTAAGGTTTCCAAGGCTAGTTTTACCCATCGCCGCAAGACCTTGTGGAATAAC
TTGACAGGTTACTTTGGTAAGACTGAAGAGGTCAAGGACAAGCTGACCAAGGCTTTGGACCAGGCAGGCTTGTCACCAAG
TGTGCGTGGGGAAGCTCTCAGCTTGGCAGAATTTGCCGGTCTAGCAGACGCACTTAAAGGGCAAGGACTCTAA

Upstream 100 bases:

>100_bases
TTCTTCATGAGGAAAGATAGTTTTTTGGTATGATTTTCATTCCCAAAATACAAGGGGAATGTGTTACAATAGTAGTAACA
GATAATAGAAAAGAGAATAG

Downstream 100 bases:

>100_bases
GATGCAGGGACAAATCATTAAAGCCTTGGCAGGTTTCTACTATGTGGAGAGTGATGGCCAGGTTTATCAAACACGCGCGC
GTGGGAATTTCCGTAAAAAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEIDDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRL
VPILADTLRDFDNVTVVNEDILKVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFFFKVSKASFTHRRKTLWNN
LTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGLADALKGQGL

Sequences:

>Translated_290_residues
MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEIDDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRL
VPILADTLRDFDNVTVVNEDILKVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFFFKVSKASFTHRRKTLWNN
LTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGLADALKGQGL
>Mature_290_residues
MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEIDDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRL
VPILADTLRDFDNVTVVNEDILKVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFFFKVSKASFTHRRKTLWNN
LTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGLADALKGQGL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=282, Percent_Identity=27.3049645390071, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI7657198, Length=225, Percent_Identity=30.6666666666667, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1786236, Length=270, Percent_Identity=32.2222222222222, Blast_Score=155, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI25141369, Length=294, Percent_Identity=27.891156462585, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25146882, Length=230, Percent_Identity=31.304347826087, Blast_Score=98, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=224, Percent_Identity=29.4642857142857, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21358017, Length=228, Percent_Identity=30.2631578947368, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21357273, Length=303, Percent_Identity=25.0825082508251, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_STRP2 (Q04II4)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817204.1
- ProteinModelPortal:   Q04II4
- SMR:   Q04II4
- STRING:   Q04II4
- EnsemblBacteria:   EBSTRT00000019979
- GeneID:   4441667
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1782
- eggNOG:   COG0030
- GeneTree:   EBGT00050000027760
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 32221; Mature: 32221

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 27-27 BINDING 29-29 BINDING 54-54 BINDING 75-75 BINDING 100-100 BINDING 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEIDDQVNVIEIGPGIGAL
CCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
TEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKVDLAQHIQNFKNPNLPI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCCCCE
KVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRISAQPNTKAYGSLSIAVQYYM
EEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEHHH
TAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFFFKVSKASFTHRRKTLWNN
HHHHHEEECCEEEECCCCCCHHHHHHHHCCCCCEEEECCCEEEEEECHHHHHHHHHHHHH
LTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGLADALKGQGL
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEIDDQVNVIEIGPGIGAL
CCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
TEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKVDLAQHIQNFKNPNLPI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHCCCCCCE
KVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRISAQPNTKAYGSLSIAVQYYM
EEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEHHH
TAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFFFKVSKASFTHRRKTLWNN
HHHHHEEECCEEEECCCCCCHHHHHHHHCCCCCEEEECCCEEEEEECHHHHHHHHHHHHH
LTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGLADALKGQGL
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA