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The map label for this gene is truB

Identifier: 116515728

GI number: 116515728

Start: 1094257

End: 1095135

Strand: Reverse

Name: truB

Synonym: SPD_1070

Alternate gene names: 116515728

Gene position: 1095135-1094257 (Counterclockwise)

Preceding gene: 116516569

Following gene: 116515672

Centisome position: 53.52

GC content: 44.94

Gene sequence:

>879_bases
ATGAACGGTATTATCAACTTAAAAAAAGAAGCAGGAATGACCTCGCATGATGCGGTTTTTAAACTGCGTAAGATTTTGGG
AACCAAGAAAATTGGTCATGGTGGGACCTTGGATCCGGATGTGGTGGGTGTTTTGCCGATTGCGGTTGGCAAGGCGACAC
GCATGGTCGAGTTTATGCAGGACGAGGGTAAGATCTATGAGGGGGAAATCATTCTGGGCTATTCCACGACGACTGAGGAT
GCTAGTGGGGAAGTGGTCGCAGAAACCCCTGTTTTGTCTTCATTGGATGAAAAGCTTGTTGATGAAGCGATTGCTAGCTT
GACTGGGCCTATTACTCAGATTCCCCCTATGTATTCGGCAGTTAAGGTTAATGGTCGCAAGCTCTATGAGTATGCGCGTG
CTGGTCAGGAAGTGGAGCGTCCAGAACGTCAGGTGATCATTTATCAATTTGAGCGAACAAGTCCGATTTCTTATGATGGC
CAACTTGCCCGATTCACTTTTCGTGTAAAATGCAGTAAAGGGACTTACATCCGTACTTTGTCAGTTGATTTGGGTGAAAA
GCTTGGTTATGCGGCTCATATGTCCCATTTGACTCGTACTAGTGCTGCTGGCTTACAATTAGAAGACGCTCTTGCCTTGG
AGGAAATTGCTGAAAAAGTAGAGGCTGGGCAATTAGATTTTCTCCATCCTTTAGAGATTGGGACAGGTGACCTTGTCAAA
GTTTTCCTAACTCCAGAAGAGGCTACAGAAGTTCGATTTGGTCGTTTTATTGAGCTAGACCAAACGGACAAAGAACTGGC
TGCCTTTGAAGATGATAAATTGTTAGCCATTCTAGAAAAACGGGGCAATCTCTATAAGCCAAGGAAGGTTTTTAGCTAG

Upstream 100 bases:

>100_bases
AATTTGTAAAAAAGAGAATAAAAGGTAGACGACGCCTCAGTAAAAATTGGCCCGGAAAAAAGCGAAGTTCGAAGCACTGA
AGGGGGAGTAGAAAGCAAAA

Downstream 100 bases:

>100_bases
ATCGTTTGGGAATAAAAATCGGGTGATAGATAACAATTGCTTGATAAAACCCCATACTAATAGTAGAATGGTTTTGGGAA
TTATAATATTCCAATTGTTG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGVLPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTED
ASGEVVAETPVLSSLDEKLVDEAIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAEKVEAGQLDFLHPLEIGTGDLVK
VFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLAILEKRGNLYKPRKVFS

Sequences:

>Translated_292_residues
MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGVLPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTED
ASGEVVAETPVLSSLDEKLVDEAIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAEKVEAGQLDFLHPLEIGTGDLVK
VFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLAILEKRGNLYKPRKVFS
>Mature_292_residues
MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGVLPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTED
ASGEVVAETPVLSSLDEKLVDEAIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAEKVEAGQLDFLHPLEIGTGDLVK
VFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLAILEKRGNLYKPRKVFS

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=264, Percent_Identity=33.7121212121212, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI215599015, Length=210, Percent_Identity=31.9047619047619, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4503337, Length=210, Percent_Identity=31.9047619047619, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI2367200, Length=248, Percent_Identity=38.7096774193548, Blast_Score=165, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17553978, Length=202, Percent_Identity=30.6930693069307, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=210, Percent_Identity=32.3809523809524, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324037, Length=193, Percent_Identity=33.6787564766839, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281364189, Length=291, Percent_Identity=28.8659793814433, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281364187, Length=291, Percent_Identity=28.8659793814433, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281364185, Length=291, Percent_Identity=28.8659793814433, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281364183, Length=291, Percent_Identity=28.8659793814433, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI62471759, Length=291, Percent_Identity=28.8659793814433, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17975520, Length=291, Percent_Identity=28.8659793814433, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_STRP2 (Q04KA7)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816541.1
- ProteinModelPortal:   Q04KA7
- SMR:   Q04KA7
- STRING:   Q04KA7
- EnsemblBacteria:   EBSTRT00000019910
- GeneID:   4441016
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1070
- eggNOG:   COG0130
- GeneTree:   EBGT00050000027943
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK02484
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015225
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 32256; Mature: 32256

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGVLPIAVGKATRMVEFMQ
CCCCEECHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEHHHHHCHHHHHHHHHH
DEGKIYEGEIILGYSTTTEDASGEVVAETPVLSSLDEKLVDEAIASLTGPITQIPPMYSA
CCCCEEECEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCE
VKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDGQLARFTFRVKCSKGTYIRTL
EEECCHHHHHHHHCCHHHCCCCEEEEEEEEECCCCCCCCCCEEEEEEEEEECCCCEEEEE
SVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAEKVEAGQLDFLHPLEIGTGDLVK
EECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
VFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLAILEKRGNLYKPRKVFS
EEECCCHHHHHHCCEEEECCCCCHHHHHCCCCCEEEEEHHCCCCCCCHHCCC
>Mature Secondary Structure
MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGVLPIAVGKATRMVEFMQ
CCCCEECHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEHHHHHCHHHHHHHHHH
DEGKIYEGEIILGYSTTTEDASGEVVAETPVLSSLDEKLVDEAIASLTGPITQIPPMYSA
CCCCEEECEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCE
VKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDGQLARFTFRVKCSKGTYIRTL
EEECCHHHHHHHHCCHHHCCCCEEEEEEEEECCCCCCCCCCEEEEEEEEEECCCCEEEEE
SVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAEKVEAGQLDFLHPLEIGTGDLVK
EECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
VFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLAILEKRGNLYKPRKVFS
EEECCCHHHHHHCCEEEECCCCCHHHHHCCCCCEEEEEHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA