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The map label for this gene is speE

Identifier: 116515725

GI number: 116515725

Start: 828605

End: 829465

Strand: Direct

Name: speE

Synonym: SPD_0811

Alternate gene names: 116515725

Gene position: 828605-829465 (Clockwise)

Preceding gene: 116516967

Following gene: 116516766

Centisome position: 40.5

GC content: 44.6

Gene sequence:

>861_bases
ATGGATTTATGGTTTTCTGAAGTTCATACTCCAGATGTCAAATTGTCTCTGAGAACAGCCAAGCAACTTTACGCTGGAAA
AAGTGAATGGCAGGATATCGAAGTCTTGGATACGCCAGCTTTTGGGAAAATACTGATTTTAAATGGCCATGTCTTGTTCT
CAGATGCGGATGATTTCGTCTACAATGAAATGACCGTCCACGTTCCCATGGCTGTCCACCCAAATCCAAAGAAAGTATTG
GTTATTGGGGGTGGTGACGGCGGTGTTGCCCAAGTATTAACACTCTATCCTGAACTGGAACAAATCGATATCGTGGAACC
GGATGAGATGTTGGTCGAGGTCTGTCGTGAGTATTTCCCAGACTTTGCTGCAGGGCTAGATGATCCTCGTGTTACCATTT
ACTACCAAAATGGGCTACGCTTTTTGCGAAACTGCGAAGATGATTACGATATTATCATCAACGATGCGACAGATCCATTT
GGCCATACGGAAGGACTCTTTACCAAGGAATTCTACGGCAATAGTTATCGAGCTCTGAAGGAAGACGGCATCATGATTTA
CCAGCATGGGAGTCCCTTCTTTGACGAGGATGAGTCGGCCTGCCGAAGCATGCACCGCAAGGTCAATCAAGCCTTTCCAA
TCAGTCGGGTTTATCAGGCCCATATTCCAACTAGCCCAGCTGGCTATTGGTTGTTTGGATTTGCATCGAAAAAATACCAC
CCTGTCAAAGATTTTGACAAGGAAGGCTGGAAAAAACGCCAGCTTTTCACAGAATACTACACTGCAAACTTACACGTGGG
AGCCTTTATGTTGCCCAAGTATGTTGAGGACATTTTAGAAGAAGAGGAAGGAAAAAAATGA

Upstream 100 bases:

>100_bases
GATTGGAGGAAATGAACTAATCCACAGTGACTTATTCCAAGTATACCACTTGGGCTTTGGCAGTAGCTAACTGCGCTAAA
TATAATATAAGGAGAAATAG

Downstream 100 bases:

>100_bases
GTCGTTTACTAGTTATTGGTTGTGGGGGCGTTGCCCAAGTTGCTATTTCAAAGATTTGTCAAGATAGCGAAACATTTACA
GAGATTATGATTGCTAGCCG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKILILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVL
VIGGGDGGVAQVLTLYPELEQIDIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVYQAHIPTSPAGYWLFGFASKKYH
PVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVEDILEEEEGKK

Sequences:

>Translated_286_residues
MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKILILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVL
VIGGGDGGVAQVLTLYPELEQIDIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVYQAHIPTSPAGYWLFGFASKKYH
PVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVEDILEEEEGKK
>Mature_286_residues
MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKILILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVL
VIGGGDGGVAQVLTLYPELEQIDIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVYQAHIPTSPAGYWLFGFASKKYH
PVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVEDILEEEEGKK

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=285, Percent_Identity=32.6315789473684, Blast_Score=179, Evalue=2e-45,
Organism=Escherichia coli, GI1786312, Length=264, Percent_Identity=35.9848484848485, Blast_Score=180, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI25154106, Length=276, Percent_Identity=33.3333333333333, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI212645616, Length=227, Percent_Identity=33.4801762114537, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325326, Length=287, Percent_Identity=32.404181184669, Blast_Score=168, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6323175, Length=290, Percent_Identity=28.9655172413793, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24645443, Length=284, Percent_Identity=33.4507042253521, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI21355941, Length=221, Percent_Identity=33.0316742081448, Blast_Score=133, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_STRPN (P66835)

Other databases:

- EMBL:   AE005672
- PIR:   A95106
- RefSeq:   NP_345402.1
- ProteinModelPortal:   P66835
- SMR:   P66835
- EnsemblBacteria:   EBSTRT00000025187
- GeneID:   930871
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_0918
- TIGR:   SP_0918
- GeneTree:   EBGT00050000029841
- HOGENOM:   HBG635113
- OMA:   VKHPLVE
- ProtClustDB:   PRK00811
- BioCyc:   SPNE170187-1:SP_0918-MONOMER
- BRENDA:   2.5.1.16
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 32859; Mature: 32859

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2; PS00962 RIBOSOMAL_S2_1

Important sites: BINDING 31-31 BINDING 86-86 BINDING 106-106 BINDING 155-155 BINDING 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKILILNGHVLFSDADDFV
CCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEECEEEEECCCCCE
YNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQIDIVEPDEMLVEVCREYFP
ECEEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
DFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPFGHTEGLFTKEFYGNSYRALK
HHHCCCCCCEEEEEECCCHHHHHCCCCCCEEEEECCCCCCCCCCCCEEHHHCCCCCEEEC
EDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVYQAHIPTSPAGYWLFGFASKKYH
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCEEEEEECCCCCC
PVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVEDILEEEEGKK
CCCCCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKILILNGHVLFSDADDFV
CCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEECEEEEECCCCCE
YNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQIDIVEPDEMLVEVCREYFP
ECEEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
DFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPFGHTEGLFTKEFYGNSYRALK
HHHCCCCCCEEEEEECCCHHHHHCCCCCCEEEEECCCCCCCCCCCCEEHHHCCCCCEEEC
EDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVYQAHIPTSPAGYWLFGFASKKYH
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCEEEEEECCCCCC
PVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVEDILEEEEGKK
CCCCCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916