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The map label for this gene is cysB [H]

Identifier: 116515669

GI number: 116515669

Start: 836265

End: 837173

Strand: Direct

Name: cysB [H]

Synonym: SPD_0818

Alternate gene names: 116515669

Gene position: 836265-837173 (Clockwise)

Preceding gene: 116516715

Following gene: 116516537

Centisome position: 40.87

GC content: 40.7

Gene sequence:

>909_bases
ATGAACATTCAACAATTACGCTATGTTGTGGCTATTGCCAATAGTGGTACTTTTCGTGAAGCTGCTGAAAAGATGTATGT
TAGTCAGCCGAGTCTGTCTATTTCTGTTCGTGATTTGGAAAAAGAGTTGGGCTTTAAGATTTTCCGTCGGACCAGCTCAG
GGACTTTCTTGACCCGTCGTGGGATGGAATTTTATGAAAAAGCGCAAGAATTGGTTAAAGGATTTGATATTTTTCAAAAT
CAGTATGCCAATCCTGAAGAAGAAAAAGATGAATTTTCCGTTGCTAGCCAGCACTATGACTTCTTACCACCAACTATTAC
GGCCTTTTCAGAGCGCTATCCTGACTATAAGAACTTCCGTATTTTTGAATCAACTACTGTTCAAATATTAGATGAAGTGG
CGCAAGGGCATAGTGAGATTGGGATTATCTACCTCAACAATCAAAATAAAAAGGGGATTATGCAACGGGTTGAAAAGTTA
GGTCTGGAGGTCATCGAATTGATTCCTTTCCATACCCATATTTATCTCTGTGAGGGTCATCCTTTAGCCCAGAAAGAGGA
ATTAGTCATGGAGGATTTAGCGGATTTACCAACGGTTCGTTTCACTCAAGAGAAAGACGAGTACCTTTATTATTCAGAGA
ACTTTGTCGATACCAGCGCTAGCTCACAGATGTTTAATGTGACAGACCGTGCCACCTTGAATGGTATTTTGGAGCGGACG
GACGCCTATGCGACAGGTTCTGGATTTTTAGATAGTGACAGTGTTAATGGCATTACAGCTATTCGTCTCAAGGATAACCT
AGATAACCGCATGGTCTATGTTAAACGTGAAGAAGTGGAGCTTAGTCAAGCTGGGACTCTCTTCGTAGAAGTCATGCAAG
AATATTTTGATCAAAAGAGGAAATCATGA

Upstream 100 bases:

>100_bases
ATAGTGAAGATAGTTAAGAAGAATAGGATGTTCTTTTTTCCTTTTTGGAAAACTTCTAAAATATGGTATAATGAAAAGAT
AAAGAAGTTGGGGGTAGAAG

Downstream 100 bases:

>100_bases
AAAAAAGAGCAATAGTGGCAGTCATTGTACTGCTTTTAATTGGGCTGGATCAGTTGGTCAAATCCTATATCGTCCAGCAG
ATTCCACTGGGTGAAGTGCG

Product: transcriptional regulator, LysR family protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELGFKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQN
QYANPEEEKDEFSVASQHYDFLPPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDTSASSQMFNVTDRATLNGILERT
DAYATGSGFLDSDSVNGITAIRLKDNLDNRMVYVKREEVELSQAGTLFVEVMQEYFDQKRKS

Sequences:

>Translated_302_residues
MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELGFKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQN
QYANPEEEKDEFSVASQHYDFLPPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDTSASSQMFNVTDRATLNGILERT
DAYATGSGFLDSDSVNGITAIRLKDNLDNRMVYVKREEVELSQAGTLFVEVMQEYFDQKRKS
>Mature_302_residues
MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELGFKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQN
QYANPEEEKDEFSVASQHYDFLPPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDTSASSQMFNVTDRATLNGILERT
DAYATGSGFLDSDSVNGITAIRLKDNLDNRMVYVKREEVELSQAGTLFVEVMQEYFDQKRKS

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=212, Percent_Identity=31.1320754716981, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790399, Length=204, Percent_Identity=27.9411764705882, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI145693105, Length=300, Percent_Identity=24, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787601, Length=141, Percent_Identity=31.9148936170213, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI157672245, Length=307, Percent_Identity=24.1042345276873, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789204, Length=199, Percent_Identity=28.643216080402, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788887, Length=196, Percent_Identity=26.530612244898, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34880; Mature: 34880

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELGFKIFRRTSSGTFLTRR
CCHHHEEEEEEEECCCCHHHHHHHHHHCCCCCEEEHHHHHHHHCHHEEEECCCCCEEECC
GMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFLPPTITAFSERYPDYKNFR
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEE
IFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKLGLEVIELIPFHTHIYLCEGH
EEHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
PLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDTSASSQMFNVTDRATLNGILERT
CCCHHHHHHHHHHHCCCCEEEECCCCCEEEEECCEECCCCCCCEEECHHHHHHHHHHHHH
DAYATGSGFLDSDSVNGITAIRLKDNLDNRMVYVKREEVELSQAGTLFVEVMQEYFDQKR
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHC
KS
CC
>Mature Secondary Structure
MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELGFKIFRRTSSGTFLTRR
CCHHHEEEEEEEECCCCHHHHHHHHHHCCCCCEEEHHHHHHHHCHHEEEECCCCCEEECC
GMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFLPPTITAFSERYPDYKNFR
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEE
IFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKLGLEVIELIPFHTHIYLCEGH
EEHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
PLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDTSASSQMFNVTDRATLNGILERT
CCCHHHHHHHHHHHCCCCEEEECCCCCEEEEECCEECCCCCCCEEECHHHHHHHHHHHHH
DAYATGSGFLDSDSVNGITAIRLKDNLDNRMVYVKREEVELSQAGTLFVEVMQEYFDQKR
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHC
KS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]