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The map label for this gene is ply

Identifier: 116515376

GI number: 116515376

Start: 1721457

End: 1722872

Strand: Reverse

Name: ply

Synonym: SPD_1726

Alternate gene names: 116515376

Gene position: 1722872-1721457 (Counterclockwise)

Preceding gene: 116516591

Following gene: 116517178

Centisome position: 84.2

GC content: 42.02

Gene sequence:

>1416_bases
ATGGCAAATAAAGCAGTAAATGACTTTATACTAGCTATGAATTACGATAAAAAGAAACTCTTGACCCATCAGGGAGAAAG
TATTGAAAATCGTTTCATCAAAGAGGGTAATCAGCTACCCGATGAGTTTGTTGTTATCGAAAGAAAGAAGCGGAGCTTGT
CGACAAATACAAGTGATATTTCTGTAACAGCTACCAACGACAGTCGCCTCTATCCTGGAGCACTTCTCGTAGTGGATGAG
ACCTTGTTAGAGAATAATCCCACTCTTCTTGCGGTTGATCGTGCTCCGATGACTTATAGTATTGATTTGCCTGGTTTGGC
AAGTAGCGATAGCTTTCTCCAAGTGGAAGACCCCAGCAATTCAAGTGTTCGCGGAGCGGTAAACGATTTGTTGGCTAAGT
GGCATCAAGATTATGGTCAGGTCAATAATGTCCCAGCTAGAATGCAGTATGAAAAAATAACGGCTCACAGCATGGAACAA
CTCAAGGTCAAGTTTGGTTCTGACTTTGAAAAGACAGGGAATTCTCTTGATATTGATTTTAACTCTGTCCATTCAGGTGA
AAAGCAGATTCAGATTGTTAATTTTAAGCAGATTTATTATACAGTCAGCGTAGACGCTGTTAAAAATCCAGGAGATGTGT
TTCAAGATACTGTAACGGTAGAGGATTTAAAACAGAGAGGAATTTCTGCAGAGCGTCCTTTGGTCTATATTTCGAGTGTT
GCTTATGGGCGCCAAGTCTATCTCAAGTTGGAAACCACGAGTAAGAGTGATGAAGTAGAGGCTGCTTTTGAAGCTTTGAT
AAAAGGAGTCAAGGTAGCTCCTCAGACAGAGTGGAAGCAGATTTTGGACAATACAGAAGTGAAGGCGGTTATTTTAGGGG
GCGACCCAAGTTCGGGTGCCCGAGTTGTAACAGGCAAGGTGGATATGGTAGAGGACTTGATTCAAGAAGGCAGTCGCTTT
ACAGCAGATCATCCAGGCTTGCCGATTTCCTATACAACTTCTTTTTTACGTGACAATGTAGTTGCGACCTTTCAAAACAG
TACAGACTATGTTGAGACTAAGGTTACAGCTTACAGAAACGGAGATTTACTGCTGGATCATAGTGGTGCCTATGTTGCCC
AATATTATATTACTTGGGATGAATTATCCTATGATCATCAAGGTAAGGAAGTCTTGACTCCTAAGGCTTGGGACAGAAAT
GGGCAGGATTTGACGGCTCACTTTACCACTAGTATTCCTTTAAAAGGGAATGTTCGTAATCTCTCTGTCAAAATTAGAGA
GTGTACCGGGCTTGCCTGGGAATGGTGGCGTACGGTTTATGAAAAAACCGATTTGCCACTAGTGCGTAAGCGGACGATTT
CTATTTGGGGAACAACTCTCTATCCTCAGGTAGAGGATAAGGTAGAAAATGACTAG

Upstream 100 bases:

>100_bases
TACGAGGAAATTTTGGATCACTTAGTCCAACCACGGCTGATTTCGCTGAACAAGTCTGAGTTTATGCAACTCAATCCAGG
AACTTATTAGGAGGTAGAAG

Downstream 100 bases:

>100_bases
GAGAGGAGAATGCTTGCGACAAAAAGAGGCGATGATCTCTCTGCGGATATCTGAAAGTTTATCTCTTGCCTAGCGATTTC
CATTGGAGTATGCTCGTGAT

Product: pneumolysin

Products: NA

Alternate protein names: Thiol-activated cytolysin

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDE
TLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSV
AYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRF
TADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTPKAWDRN
GQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND

Sequences:

>Translated_471_residues
MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDE
TLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSV
AYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRF
TADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTPKAWDRN
GQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
>Mature_470_residues
ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDET
LLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQL
KVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVA
YGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFT
ADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTPKAWDRNG
QDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND

Specific function: Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation o

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host cell membrane; Multi-pass membrane protein (By similarity). Note=Secreted as soluble protein that then inserts into the host cell membrane and forms pores formed by transmembrane beta-strands (By similarity)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiol-activated cytolysin family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TACY_STRP2 (Q04IN8)

Other databases:

- EMBL:   X52474
- EMBL:   M17717
- EMBL:   CP000410
- RefSeq:   YP_817150.1
- ProteinModelPortal:   Q04IN8
- SMR:   Q04IN8
- STRING:   Q04IN8
- EnsemblBacteria:   EBSTRT00000019086
- GeneID:   4441611
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1726
- eggNOG:   NOG04845
- GeneTree:   EBGT00050000028744
- HOGENOM:   HBG337470
- OMA:   GAYVAQF
- ProtClustDB:   CLSK877006
- GO:   GO:0019835
- GO:   GO:0009405
- InterPro:   IPR001869
- Gene3D:   G3DSA:3.90.840.10
- PRINTS:   PR01400

Pfam domain/function: PF01289 Thiol_cytolysin; SSF56978 Thiol_cytolysin

EC number: NA

Molecular weight: Translated: 52900; Mature: 52769

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00481 THIOL_CYTOLYSINS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDI
CCCCCCCCEEEEECCCHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCCEE
SVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSN
EEEECCCCEECCCEEEEEEHHHHCCCCCEEEEECCCEEEEEECCCCCCCCCEEEEECCCC
SSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDF
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCHHHCCCEEEEEE
NSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSV
CCCCCCCCEEEEEEEEEEEEEEEEECCCCCCHHHHCCEEHHHHHHCCCCCCCCEEEEEEE
AYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGA
CCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCC
RVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRN
EEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCEEEEEEEEEEC
GDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRN
CCEEEECCCCEEEEEEEEEHHCCCCCCCCEECCCCCCCCCCCEEEEEEEEECCCCCCCEE
LSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
EEEEEEECCCCHHHHHHHHHHCCCCCEEHEEEEEEEEEEECCCHHHCCCCC
>Mature Secondary Structure 
ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDI
CCCCCCCEEEEECCCHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCCEE
SVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSN
EEEECCCCEECCCEEEEEEHHHHCCCCCEEEEECCCEEEEEECCCCCCCCCEEEEECCCC
SSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDF
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCHHHCCCEEEEEE
NSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSV
CCCCCCCCEEEEEEEEEEEEEEEEECCCCCCHHHHCCEEHHHHHHCCCCCCCCEEEEEEE
AYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGA
CCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCC
RVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRN
EEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCEEEEEEEEEEC
GDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRN
CCEEEECCCCEEEEEEEEEHHCCCCCCCCEECCCCCCCCCCCEEEEEEEEECCCCCCCEE
LSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
EEEEEEECCCCHHHHHHHHHHCCCCCEEHEEEEEEEEEEECCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3552992