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The map label for this gene is gidA [H]

Identifier: 116515312

GI number: 116515312

Start: 132343

End: 134256

Strand: Direct

Name: gidA [H]

Synonym: SPD_0129

Alternate gene names: 116515312

Gene position: 132343-134256 (Clockwise)

Preceding gene: 116516444

Following gene: 116516776

Centisome position: 6.47

GC content: 46.34

Gene sequence:

>1914_bases
ATGATTTATCATTTTACTGAAGAATACGATATTATTGTAATTGGTGCGGGACACGCTGGGGTTGAGGCTTCCTTGGCCGC
TAGCCGTATGGGCTGTAAGGTCCTGCTTGCGACCATCAATATTGAAATGCTGGCTTTCATGCCTTGTAATCCCTCTATCG
GTGGTTCTGCCAAGGGGATTGTCGTACGTGAAGTCGATGCCCTCGGTGGCGAGATGGCCAAGACTATTGACAAGACTTAC
ATCCAGATGAAGATGCTCAACACAGGGAAGGGGCCAGCTGTCCGTGCTCTTCGTGCGCAGGCTGACAAGGAACTTTACTC
TAAGGAGATGCGCAAGACAGTTGAAAATCAAGAAAATCTGACCCTTCGTCAAACCATGATTGATGAGATTTTGGTGGAAG
ATGGCAAGGCTGTCGGTGTGCGTACAGCCACCCATCAAGAATATGCTGCTAAGGCTGTTATTGTGACGACAGGGACTGCT
CTCCGTGGGGAAATTATCATCGGAGACCTCAAGTACTCATCAGGTCCTAACCACAGCTTGGCTTCTATTAACCTAGCTGA
CAATCTCAAGGAACTGGGTCTCGAAATCGGTCGTTTCAAGACAGGAACCCCTCCACGTGTCAAGGCTTCTTCTATCAATT
ACGATGTGACAGAAATTCAGCCAGGAGACGAAGTGCCTAATCATTTCTCATACACTTCACGTGATGAGGATTATGTCAAG
GACCAAGTACCATGCTGGTTGACCTATACCAATGGTACCAGTCATGAGATTATCCAAAACAACCTCCACCGTGCGCCTAT
GTTTACAGGTGTGGTCAAGGGAGTGGGACCTCGTTACTGTCCATCGATTGAGGACAAGATTGTGCGCTTTGCGGACAAGG
AACGTCACCAACTCTTCCTTGAGCCAGAAGGACGCAATACAGAGGAAGTCTATGTTCAAGGACTTTCAACCAGTCTGCCT
GAGGATGTCCAACGTGACTTGGTTCATTCCATCAAAGGTCTGGAAAATGCAGAGATGATGCGAACAGGTTATGCTATTGA
GTATGATATGGTCTTGCCTCATCAGTTGCGTGCGACTTTGGAAACCAAGAAAATCTCAGGTCTCTTCACTGCTGGTCAGA
CAAATGGAACGTCAGGTTACGAAGAGGCAGCAGGCCAAGGGATTATCGCGGGTATCAATGCGGCTCTGAAAATCCAAGGC
AAGCCTGAATTGATTCTAAAACGAAGTGACGGTTATATCGGGGTGATGATCGACGACTTGGTGACCAAGGGAACCATTGA
ACCTTACCGTCTCTTGACCAGTCGTGCTGAATATCGTCTCATTCTTCGCCATGACAATGCTGATATGCGCTTGACTGAGA
TGGGCCGTGAGATTGGCCTTGTGGACGATGAACGCTGGGCTCGTTTTGAAATCAAGAAAAATCAATTTGATAATGAGATG
AAGCGTCTAGACAGTATCAAACTTAAGCCAGTCAAGGAAACCAATGCTAAGGTTGAGGAAATGGGCTTCAAGCCGTTGAC
AGATGCGGTGACAGCCAAGGAATTCCTTCGCCGTCCAGAAGTTTCTTACCAAGATGTGGTGGCCTTCATCGGACCAGCTG
CAGAGGAATTAGATGACAAGATTATCGAATTGATTGAAACAGAAATCAAGTACGAAGGCTATATTTCCAAAGCCATGGAT
CAGGTTGCCAAGATGAAACGTATGGAAGAAAAACGCATTCCAGCCAATATCGACTGGGATGACATCGATTCTATTGCGAC
GGAAGCTCGTCAGAAGTTCAAACTCATCAATCCAGAAACCATCGGCCAAGCCAGTCGTATTTCGGGAGTAAACCCAGCAG
ATATTTCTATTTTGATGGTGTATCTGGAAGGTAAAAATCGTAGTATTTCTAAAACTCTTCAAAAATCAAAATGA

Upstream 100 bases:

>100_bases
GCTTGAGGATATCCAGCTAGTTCCAGCCTTTTTAAAAACAGCCCTACCAGATTGGGAAGGCCAACTAAGACACATTCATC
TTGAGGAATAGGAGAGAAAC

Downstream 100 bases:

>100_bases
TACGTCGTCGGCTTCTTACGAATGAGTTCAAAGCTTGGCTTTGATTCATCTCCAACCTCCCATAGTTCCCCGAACTATGG
GAGCTAACTCAAGTTATGCA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MIYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEMLAFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTY
IQMKMLNTGKGPAVRALRAQADKELYSKEMRKTVENQENLTLRQTMIDEILVEDGKAVGVRTATHQEYAAKAVIVTTGTA
LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTEIQPGDEVPNHFSYTSRDEDYVK
DQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVGPRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLP
EDVQRDLVHSIKGLENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGINAALKIQG
KPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMRLTEMGREIGLVDDERWARFEIKKNQFDNEM
KRLDSIKLKPVKETNAKVEEMGFKPLTDAVTAKEFLRRPEVSYQDVVAFIGPAAEELDDKIIELIETEIKYEGYISKAMD
QVAKMKRMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEGKNRSISKTLQKSK

Sequences:

>Translated_637_residues
MIYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEMLAFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTY
IQMKMLNTGKGPAVRALRAQADKELYSKEMRKTVENQENLTLRQTMIDEILVEDGKAVGVRTATHQEYAAKAVIVTTGTA
LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTEIQPGDEVPNHFSYTSRDEDYVK
DQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVGPRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLP
EDVQRDLVHSIKGLENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGINAALKIQG
KPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMRLTEMGREIGLVDDERWARFEIKKNQFDNEM
KRLDSIKLKPVKETNAKVEEMGFKPLTDAVTAKEFLRRPEVSYQDVVAFIGPAAEELDDKIIELIETEIKYEGYISKAMD
QVAKMKRMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEGKNRSISKTLQKSK
>Mature_637_residues
MIYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEMLAFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTY
IQMKMLNTGKGPAVRALRAQADKELYSKEMRKTVENQENLTLRQTMIDEILVEDGKAVGVRTATHQEYAAKAVIVTTGTA
LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTEIQPGDEVPNHFSYTSRDEDYVK
DQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVGPRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLP
EDVQRDLVHSIKGLENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGINAALKIQG
KPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMRLTEMGREIGLVDDERWARFEIKKNQFDNEM
KRLDSIKLKPVKETNAKVEEMGFKPLTDAVTAKEFLRRPEVSYQDVVAFIGPAAEELDDKIIELIETEIKYEGYISKAMD
QVAKMKRMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEGKNRSISKTLQKSK

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=648, Percent_Identity=39.9691358024691, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI19882217, Length=673, Percent_Identity=38.4843982169391, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI183227703, Length=688, Percent_Identity=37.6453488372093, Blast_Score=419, Evalue=1e-117,
Organism=Escherichia coli, GI2367273, Length=619, Percent_Identity=50.0807754442649, Blast_Score=623, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI17534255, Length=622, Percent_Identity=38.9067524115756, Blast_Score=447, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6321202, Length=625, Percent_Identity=39.84, Blast_Score=450, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24658174, Length=636, Percent_Identity=41.1949685534591, Blast_Score=442, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 71045; Mature: 71045

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEMLAFMPCNPSIGGSAKGI
CEEEEECCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEECEEEEEEECCCCCCCCCCCCE
VVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQADKELYSKEMRKTVENQENL
EEEEHHHHCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TLRQTMIDEILVEDGKAVGVRTATHQEYAAKAVIVTTGTALRGEIIIGDLKYSSGPNHSL
HHHHHHHHHHHHCCCCEEEEEECCCHHHHCEEEEEEECCEEECEEEEEEEECCCCCCCCE
ASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTEIQPGDEVPNHFSYTSRDEDYVK
EEEEHHHHHHHHCHHHCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHH
DQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVGPRYCPSIEDKIVRFADKERHQLFL
CCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEE
EPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGLENAEMMRTGYAIEYDMVLPHQLRATL
CCCCCCCHHHEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEEEEECHHHHHHHH
ETKKISGLFTAGQTNGTSGYEEAAGQGIIAGINAALKIQGKPELILKRSDGYIGVMIDDL
HHHHHCEEEECCCCCCCCCHHHHCCCCEEEECCEEEEECCCCCEEEEECCCEEEEEEHHH
VTKGTIEPYRLLTSRAEYRLILRHDNADMRLTEMGREIGLVDDERWARFEIKKNQFDNEM
HCCCCCCHHHHHHCCCCEEEEEEECCCCEEHHHCCCCCCCCCCCCCEEEEEEHHHHHHHH
KRLDSIKLKPVKETNAKVEEMGFKPLTDAVTAKEFLRRPEVSYQDVVAFIGPAAEELDDK
HHHHCCCCCCCHHCCCHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHH
IIELIETEIKYEGYISKAMDQVAKMKRMEEKRIPANIDWDDIDSIATEARQKFKLINPET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCCCHH
IGQASRISGVNPADISILMVYLEGKNRSISKTLQKSK
CCCHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MIYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEMLAFMPCNPSIGGSAKGI
CEEEEECCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEECEEEEEEECCCCCCCCCCCCE
VVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQADKELYSKEMRKTVENQENL
EEEEHHHHCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TLRQTMIDEILVEDGKAVGVRTATHQEYAAKAVIVTTGTALRGEIIIGDLKYSSGPNHSL
HHHHHHHHHHHHCCCCEEEEEECCCHHHHCEEEEEEECCEEECEEEEEEEECCCCCCCCE
ASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTEIQPGDEVPNHFSYTSRDEDYVK
EEEEHHHHHHHHCHHHCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHH
DQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVGPRYCPSIEDKIVRFADKERHQLFL
CCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEE
EPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGLENAEMMRTGYAIEYDMVLPHQLRATL
CCCCCCCHHHEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEEEEECHHHHHHHH
ETKKISGLFTAGQTNGTSGYEEAAGQGIIAGINAALKIQGKPELILKRSDGYIGVMIDDL
HHHHHCEEEECCCCCCCCCHHHHCCCCEEEECCEEEEECCCCCEEEEECCCEEEEEEHHH
VTKGTIEPYRLLTSRAEYRLILRHDNADMRLTEMGREIGLVDDERWARFEIKKNQFDNEM
HCCCCCCHHHHHHCCCCEEEEEEECCCCEEHHHCCCCCCCCCCCCCEEEEEEHHHHHHHH
KRLDSIKLKPVKETNAKVEEMGFKPLTDAVTAKEFLRRPEVSYQDVVAFIGPAAEELDDK
HHHHCCCCCCCHHCCCHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHH
IIELIETEIKYEGYISKAMDQVAKMKRMEEKRIPANIDWDDIDSIATEARQKFKLINPET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEECCCCHH
IGQASRISGVNPADISILMVYLEGKNRSISKTLQKSK
CCCHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA