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The map label for this gene is miaA

Identifier: 116515286

GI number: 116515286

Start: 391266

End: 392204

Strand: Reverse

Name: miaA

Synonym: BCc_371

Alternate gene names: 116515286

Gene position: 392204-391266 (Counterclockwise)

Preceding gene: 116515287

Following gene: 116515285

Centisome position: 94.19

GC content: 17.78

Gene sequence:

>939_bases
ATGAATAAAAAAAAATTTTTGTTTTTCTTAATGGGTCCTACAGCAATAGGTAAAAGTTCATTAGCTTTAGAAATTAAAAA
AAAATTTCCTTTAATAGAATTAATTAGTGTAGATTCTAAATTAGTATATAAAGGATTAAATATTGGTACTGATAAACCAA
ATAAATCTGATTTAAAAAATTTTTCTTATAAATTAGTTAATATTGTAAAACCTAAAAATATTTATACTGTAATAAATTTT
TATAATGATGTTTTAAAAGAAATAAAAAATATATTAAAATCTGGAAAAATTCCCCTATTGGTGGGTGGTACCATGTTATA
TTTTAAAATTTTATTAAACGGATTTGCTAATTTACCTCCTTCTAATTCTATAATTAGAAAATATATATTTAAAAATATTT
GTTTAAAAAAAAAAAAAAATTTATTTAATTTATTAAAAAAAATAGATCCGATTTCTAGTAAAAAAATTCATATTAATGAT
GTTCAACGTGTTTTAAGAGCAGTTGAAGTTTTTTTTGTATCTGGTGGTTTTCCATTAAGTGAATTAATTAAATTTTTTCA
TAATAAATTACCATATAAAGTTTTTCAGTTTGGTTTAATTCCAGATAATAAAGAGCATTTATATAAAAAAATAGAAAAAA
GATTTTTTTTTATGTTAAAAAGTGGATTTAAAAAAGAAGTACAAAATTTATATAATCAGAAATTTTTAGATCCGAAATTA
CCATCAATGAATAGTATTGGTTATAAACAAATGTTATTATATTTAAAAAATAAATATACATATTTTCAAATGATTAAAGA
AACAATTAAATCTACTCATAAATTAGTAAAACATCAATTAACATGGTTGAAAAAATGGCCAAATATTATTTTTATTAAAG
ACAATAAAAAAGATTTATTAATTACTAAAATATATAAAATATTAAATAGAAATTTATAA

Upstream 100 bases:

>100_bases
AATTAATAAAATATTAATTAATAATTAAATTTTAATTAAATTATTAATTAATTTTTAAAATACTTAATAAATATTCAGTA
TTAAAAGAATATATAAAGAT

Downstream 100 bases:

>100_bases
AATGAAATAGTTGGGTATTCTTTTAAAAATTTTAAATTTATAAATTATTTTTTAAAAAATATATTTTTTTTTATAAAAAG
TAATATATTAATGATATAAT

Product: hypothetical protein

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNKKKFLFFLMGPTAIGKSSLALEIKKKFPLIELISVDSKLVYKGLNIGTDKPNKSDLKNFSYKLVNIVKPKNIYTVINF
YNDVLKEIKNILKSGKIPLLVGGTMLYFKILLNGFANLPPSNSIIRKYIFKNICLKKKKNLFNLLKKIDPISSKKIHIND
VQRVLRAVEVFFVSGGFPLSELIKFFHNKLPYKVFQFGLIPDNKEHLYKKIEKRFFFMLKSGFKKEVQNLYNQKFLDPKL
PSMNSIGYKQMLLYLKNKYTYFQMIKETIKSTHKLVKHQLTWLKKWPNIIFIKDNKKDLLITKIYKILNRNL

Sequences:

>Translated_312_residues
MNKKKFLFFLMGPTAIGKSSLALEIKKKFPLIELISVDSKLVYKGLNIGTDKPNKSDLKNFSYKLVNIVKPKNIYTVINF
YNDVLKEIKNILKSGKIPLLVGGTMLYFKILLNGFANLPPSNSIIRKYIFKNICLKKKKNLFNLLKKIDPISSKKIHIND
VQRVLRAVEVFFVSGGFPLSELIKFFHNKLPYKVFQFGLIPDNKEHLYKKIEKRFFFMLKSGFKKEVQNLYNQKFLDPKL
PSMNSIGYKQMLLYLKNKYTYFQMIKETIKSTHKLVKHQLTWLKKWPNIIFIKDNKKDLLITKIYKILNRNL
>Mature_312_residues
MNKKKFLFFLMGPTAIGKSSLALEIKKKFPLIELISVDSKLVYKGLNIGTDKPNKSDLKNFSYKLVNIVKPKNIYTVINF
YNDVLKEIKNILKSGKIPLLVGGTMLYFKILLNGFANLPPSNSIIRKYIFKNICLKKKKNLFNLLKKIDPISSKKIHIND
VQRVLRAVEVFFVSGGFPLSELIKFFHNKLPYKVFQFGLIPDNKEHLYKKIEKRFFFMLKSGFKKEVQNLYNQKFLDPKL
PSMNSIGYKQMLLYLKNKYTYFQMIKETIKSTHKLVKHQLTWLKKWPNIIFIKDNKKDLLITKIYKILNRNL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=308, Percent_Identity=28.2467532467532, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1790613, Length=302, Percent_Identity=41.3907284768212, Blast_Score=282, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI25144712, Length=271, Percent_Identity=28.7822878228782, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324848, Length=317, Percent_Identity=29.3375394321767, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24649830, Length=275, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BUCCC (Q056X7)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802915.1
- ProteinModelPortal:   Q056X7
- SMR:   Q056X7
- STRING:   Q056X7
- EnsemblBacteria:   EBBUCT00000001318
- GeneID:   4440962
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_371
- eggNOG:   COG0324
- GeneTree:   EBGT00050000008032
- HOGENOM:   HBG559459
- OMA:   LPYTVAN
- BioCyc:   BAPH372461:BCC_371-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36687; Mature: 36687

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKKFLFFLMGPTAIGKSSLALEIKKKFPLIELISVDSKLVYKGLNIGTDKPNKSDLKN
CCCCEEEEEEECCCCCCCCHHEEEHHHCCCEEEEHHCCHHHHHCCCCCCCCCCCHHHHHH
FSYKLVNIVKPKNIYTVINFYNDVLKEIKNILKSGKIPLLVGGTMLYFKILLNGFANLPP
HHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHCCCCC
SNSIIRKYIFKNICLKKKKNLFNLLKKIDPISSKKIHINDVQRVLRAVEVFFVSGGFPLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCHH
ELIKFFHNKLPYKVFQFGLIPDNKEHLYKKIEKRFFFMLKSGFKKEVQNLYNQKFLDPKL
HHHHHHHCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSMNSIGYKQMLLYLKNKYTYFQMIKETIKSTHKLVKHQLTWLKKWPNIIFIKDNKKDLL
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHH
ITKIYKILNRNL
HHHHHHHHCCCC
>Mature Secondary Structure
MNKKKFLFFLMGPTAIGKSSLALEIKKKFPLIELISVDSKLVYKGLNIGTDKPNKSDLKN
CCCCEEEEEEECCCCCCCCHHEEEHHHCCCEEEEHHCCHHHHHCCCCCCCCCCCHHHHHH
FSYKLVNIVKPKNIYTVINFYNDVLKEIKNILKSGKIPLLVGGTMLYFKILLNGFANLPP
HHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHCCCCC
SNSIIRKYIFKNICLKKKKNLFNLLKKIDPISSKKIHINDVQRVLRAVEVFFVSGGFPLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCHH
ELIKFFHNKLPYKVFQFGLIPDNKEHLYKKIEKRFFFMLKSGFKKEVQNLYNQKFLDPKL
HHHHHHHCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSMNSIGYKQMLLYLKNKYTYFQMIKETIKSTHKLVKHQLTWLKKWPNIIFIKDNKKDLL
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHH
ITKIYKILNRNL
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA