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The map label for this gene is def

Identifier: 116515231

GI number: 116515231

Start: 354044

End: 354493

Strand: Direct

Name: def

Synonym: BCc_313

Alternate gene names: 116515231

Gene position: 354044-354493 (Clockwise)

Preceding gene: 116515228

Following gene: 116515232

Centisome position: 85.03

GC content: 21.78

Gene sequence:

>450_bases
ATGTCAATACGTAAAATACTACAATTTCCAGATTATCGTTTACGATTACTATCTAAACCTATTAAGATTATAAATAAAAA
AACAAAAAAAATAATCTATGATATGTTTGATACAATGTATGCTAATAATGGTATTGGGTTAGCTGCTCCCCAAATAAATA
TTTTGAAACAAATCATTGTTATTAGTTCTCTAAAACCTACAATGTCTGAATTAGTTTTAATTAATCCAGTAATTTTAAAA
AAAAATAAAAAATATATAAATACAATTGAAGGTTGTTTATCAATTCCTAAAAAAACTGCTAAAATAAAAAGATCTAGTTG
TATAAAAATACAAGCAATAAATACTTACGGAAAATCATTTACATTAACAGCAAAATCATTGCTATCAATATGCATTCAAC
ATGAAATAGATCATTTAATTGGAAAATTATTTATAGATTATATTAATTAA

Upstream 100 bases:

>100_bases
AATAAATAGTTAATTAAATATTCTGGTAAATTAACTTAAAATTTAATAAAAATATTTTATTTATATAAATATTTATAATA
AAATAATATAAATAATATTT

Downstream 100 bases:

>100_bases
TACCAAAATTTTCTATAATAATTATTATTAAACATATGTTAAAAAAAAAAACAAAAATAATTTTTGCTGGAAGCGATAAA
TTTTCATTAGCACATCTTCG

Product: hypothetical protein

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MSIRKILQFPDYRLRLLSKPIKIINKKTKKIIYDMFDTMYANNGIGLAAPQINILKQIIVISSLKPTMSELVLINPVILK
KNKKYINTIEGCLSIPKKTAKIKRSSCIKIQAINTYGKSFTLTAKSLLSICIQHEIDHLIGKLFIDYIN

Sequences:

>Translated_149_residues
MSIRKILQFPDYRLRLLSKPIKIINKKTKKIIYDMFDTMYANNGIGLAAPQINILKQIIVISSLKPTMSELVLINPVILK
KNKKYINTIEGCLSIPKKTAKIKRSSCIKIQAINTYGKSFTLTAKSLLSICIQHEIDHLIGKLFIDYIN
>Mature_148_residues
SIRKILQFPDYRLRLLSKPIKIINKKTKKIIYDMFDTMYANNGIGLAAPQINILKQIIVISSLKPTMSELVLINPVILKK
NKKYINTIEGCLSIPKKTAKIKRSSCIKIQAINTYGKSFTLTAKSLLSICIQHEIDHLIGKLFIDYIN

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=48.993288590604, Blast_Score=159, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_BUCCC (Q057D2)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802860.1
- ProteinModelPortal:   Q057D2
- SMR:   Q057D2
- STRING:   Q057D2
- EnsemblBacteria:   EBBUCT00000001232
- GeneID:   4440671
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_313
- eggNOG:   COG0242
- GeneTree:   EBGT00050000008071
- HOGENOM:   HBG665227
- OMA:   YINTIEG
- BioCyc:   BAPH372461:BCC_313-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 16979; Mature: 16848

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: NA

Important sites: ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRKILQFPDYRLRLLSKPIKIINKKTKKIIYDMFDTMYANNGIGLAAPQINILKQIIV
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ISSLKPTMSELVLINPVILKKNKKYINTIEGCLSIPKKTAKIKRSSCIKIQAINTYGKSF
HHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCEE
TLTAKSLLSICIQHEIDHLIGKLFIDYIN
EHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIRKILQFPDYRLRLLSKPIKIINKKTKKIIYDMFDTMYANNGIGLAAPQINILKQIIV
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ISSLKPTMSELVLINPVILKKNKKYINTIEGCLSIPKKTAKIKRSSCIKIQAINTYGKSF
HHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCEE
TLTAKSLLSICIQHEIDHLIGKLFIDYIN
EHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA