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The map label for this gene is truB [H]

Identifier: 116515162

GI number: 116515162

Start: 265222

End: 266157

Strand: Reverse

Name: truB [H]

Synonym: BCc_229

Alternate gene names: 116515162

Gene position: 266157-265222 (Counterclockwise)

Preceding gene: 116515163

Following gene: 116515161

Centisome position: 63.92

GC content: 20.62

Gene sequence:

>936_bases
ATGGTTTTTCAAAATAATAAAAATTATAATGGAATATTATTATTAGATAAACCAAAAGGTATTTCTTCTAATGGAATATT
ACAACAGATAAAAAAAATTTTTTTAGCTAAAAAAGCTGGATACACTGGATCTTTAGACCCTATGGCTACAGGAATGTTAC
CCATTTTATTTGGTAATGCGACTAAATTTTCTAAATATTTAACTGATTCAGCAAAAAAATATCATGTTATAGCAAAATTA
GGAGAAACTACTTCTACGGGTGATTTATCTGGTAAAAAATTAAAAATTAAATCTATTAATATAAATACTAATGATATTTA
TAAAATTTTAAATTCTTTTATTGGAGAAATTTATCAAGTTCCTCCTATGTTTTCTGCGATTAAATATAATGGAATTCCAT
TATATAAATATGCTCGATTAGGAATTTTAGTTCCAAGAAAAAAAAGAAAACTATTTATTTATAAAATAAATTTTATTCAA
TTTGTTAACCCTTTTTTAGAATTTACAGTAATATGTTCTAAAGGAACCTATATAAGAAGTTTAGTAAATGATATTGGTAA
TAAATTATTTTGCGGAGGACATGTAATTTTTTTACGTCGATTATATATAAAACCTTATAATTCATCAAAATTAGTAACAT
TATCAAATTTACATTTTATAGAAAATAAAAATATAGAACAAATACATAAATTTTATTTGTTTAATATGTTAAATACATTT
TTATTACCTGTAAGTTCTTTTTTTTTTAAGTATCCTATAATAAAAATATTACATTCTGATATAAATAACTTTCAAAAAAA
AATAAAGATTGCGTTGAATTTCTTTTCTACTATTGGATTAGTTCAAGTTATAACTAAAAAAAATAATATATTTTTAGGAA
TAGGTAAAATTAATAAATTTGGAATTTTAACTCCAAAATGTGTTTTATGTAGTTAA

Upstream 100 bases:

>100_bases
TACTTCCCTTATTAATGGTATTTTTATTTCTGAATTAATTAAAAATATTTAATTAATTTAGTAATAAATAATATCCTAAT
TATATATTATAGGTTAATGA

Downstream 100 bases:

>100_bases
TTTTTATTATTTAAATAATATTTTTATATAAAGTATATTACAAATTAAATATAGGATATATATGCTAAAAGATACATTAA
AAGTAAAGAATTTAGTAATG

Product: hypothetical protein

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MVFQNNKNYNGILLLDKPKGISSNGILQQIKKIFLAKKAGYTGSLDPMATGMLPILFGNATKFSKYLTDSAKKYHVIAKL
GETTSTGDLSGKKLKIKSININTNDIYKILNSFIGEIYQVPPMFSAIKYNGIPLYKYARLGILVPRKKRKLFIYKINFIQ
FVNPFLEFTVICSKGTYIRSLVNDIGNKLFCGGHVIFLRRLYIKPYNSSKLVTLSNLHFIENKNIEQIHKFYLFNMLNTF
LLPVSSFFFKYPIIKILHSDINNFQKKIKIALNFFSTIGLVQVITKKNNIFLGIGKINKFGILTPKCVLCS

Sequences:

>Translated_311_residues
MVFQNNKNYNGILLLDKPKGISSNGILQQIKKIFLAKKAGYTGSLDPMATGMLPILFGNATKFSKYLTDSAKKYHVIAKL
GETTSTGDLSGKKLKIKSININTNDIYKILNSFIGEIYQVPPMFSAIKYNGIPLYKYARLGILVPRKKRKLFIYKINFIQ
FVNPFLEFTVICSKGTYIRSLVNDIGNKLFCGGHVIFLRRLYIKPYNSSKLVTLSNLHFIENKNIEQIHKFYLFNMLNTF
LLPVSSFFFKYPIIKILHSDINNFQKKIKIALNFFSTIGLVQVITKKNNIFLGIGKINKFGILTPKCVLCS
>Mature_311_residues
MVFQNNKNYNGILLLDKPKGISSNGILQQIKKIFLAKKAGYTGSLDPMATGMLPILFGNATKFSKYLTDSAKKYHVIAKL
GETTSTGDLSGKKLKIKSININTNDIYKILNSFIGEIYQVPPMFSAIKYNGIPLYKYARLGILVPRKKRKLFIYKINFIQ
FVNPFLEFTVICSKGTYIRSLVNDIGNKLFCGGHVIFLRRLYIKPYNSSKLVTLSNLHFIENKNIEQIHKFYLFNMLNTF
LLPVSSFFFKYPIIKILHSDINNFQKKIKIALNFFSTIGLVQVITKKNNIFLGIGKINKFGILTPKCVLCS

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=234, Percent_Identity=33.3333333333333, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI215599015, Length=175, Percent_Identity=27.4285714285714, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4503337, Length=175, Percent_Identity=27.4285714285714, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI2367200, Length=304, Percent_Identity=41.7763157894737, Blast_Score=268, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17553978, Length=271, Percent_Identity=25.830258302583, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=259, Percent_Identity=26.6409266409266, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324037, Length=188, Percent_Identity=29.7872340425532, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281364189, Length=272, Percent_Identity=24.6323529411765, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281364187, Length=272, Percent_Identity=24.6323529411765, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281364185, Length=272, Percent_Identity=24.6323529411765, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281364183, Length=272, Percent_Identity=24.6323529411765, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI62471759, Length=272, Percent_Identity=24.6323529411765, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17975520, Length=272, Percent_Identity=24.6323529411765, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35430; Mature: 35430

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFQNNKNYNGILLLDKPKGISSNGILQQIKKIFLAKKAGYTGSLDPMATGMLPILFGNA
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHEEECCH
TKFSKYLTDSAKKYHVIAKLGETTSTGDLSGKKLKIKSININTNDIYKILNSFIGEIYQV
HHHHHHHHCCCHHEEEEEEECCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCC
PPMFSAIKYNGIPLYKYARLGILVPRKKRKLFIYKINFIQFVNPFLEFTVICSKGTYIRS
CCCHHHHEECCCCEEHHHHCCEEECCCCCEEEEEEEHHHHHHHHHHHEEEEECCCHHHHH
LVNDIGNKLFCGGHVIFLRRLYIKPYNSSKLVTLSNLHFIENKNIEQIHKFYLFNMLNTF
HHHHHCCEEEECCHHHEEHHHHCCCCCCCCEEEEECEEEECCCCHHHHHHHHHHHHHHHH
LLPVSSFFFKYPIIKILHSDINNFQKKIKIALNFFSTIGLVQVITKKNNIFLGIGKINKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCC
GILTPKCVLCS
CCCCCCEEECC
>Mature Secondary Structure
MVFQNNKNYNGILLLDKPKGISSNGILQQIKKIFLAKKAGYTGSLDPMATGMLPILFGNA
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHEEECCH
TKFSKYLTDSAKKYHVIAKLGETTSTGDLSGKKLKIKSININTNDIYKILNSFIGEIYQV
HHHHHHHHCCCHHEEEEEEECCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCC
PPMFSAIKYNGIPLYKYARLGILVPRKKRKLFIYKINFIQFVNPFLEFTVICSKGTYIRS
CCCHHHHEECCCCEEHHHHCCEEECCCCCEEEEEEEHHHHHHHHHHHEEEEECCCHHHHH
LVNDIGNKLFCGGHVIFLRRLYIKPYNSSKLVTLSNLHFIENKNIEQIHKFYLFNMLNTF
HHHHHCCEEEECCHHHEEHHHHCCCCCCCCEEEEECEEEECCCCHHHHHHHHHHHHHHHH
LLPVSSFFFKYPIIKILHSDINNFQKKIKIALNFFSTIGLVQVITKKNNIFLGIGKINKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCC
GILTPKCVLCS
CCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]