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The map label for this gene is tpiA

Identifier: 116515124

GI number: 116515124

Start: 221127

End: 221891

Strand: Reverse

Name: tpiA

Synonym: BCc_188

Alternate gene names: 116515124

Gene position: 221891-221127 (Counterclockwise)

Preceding gene: 116515125

Following gene: 116515118

Centisome position: 53.29

GC content: 22.61

Gene sequence:

>765_bases
ATGATAAAACCAATAATTGTAGGGAATTGGAAATTAAATGGAAGTAAAAAATTAATTGAAAGTTTTTTAAAACAATTAAA
TCAATTTTTAATTAAATATTACAAAATATGTACTGTTGTTATTGCGCCGCCTGTTTTATATTCTCATTTAATTCAAAATA
TGTTTTCTTCTGATAAAAAAAATTTTTTTTTAGGCGCCCAAAATATTGATATTCATTTTTCTGGACCATTTACCGGAGAA
ATTTCTCCTATTATGTTAAAAGATATTGGAATAAAATATGTTATTATTGGTCATTCAGAAAGACGATTAAATCATAAAGA
AACAAATGAAATAATTGCAAAAAAATTTTGTATGTTAAAAGAAGAAAATTTAGTTCCTATATTATGTATTGGAGAAACAA
AAAAAGAAAAAATTGATAAAAAAACTAAAGATATATGTAAAAAACAAATTGATATTATATTTAATATGTATGGTTCTAAT
GCATTTGATAATTCTATTATCGCATATGAACCTATTTGGTCGATTGGTTCAGGTAATTCAGCAGCACCAAAAGAAGTTCA
ATGTATTGCACATTTTATACGTAATTATGTAAAAAGTAAATCAAATCATAATATTGAAAATTTTTTTATTCAATATGGAG
GATCTGTTACAAAAAATAATGCAAAAGAACTAATTTATCAAAAAGACATTGATGGTTTTTTAGTAGGTGGAGCATCTTTA
CAATTAGAAGAATTTTCTAAAATTATAGAAATAACAAATATTTAA

Upstream 100 bases:

>100_bases
ATTTTTAATATATACGAGTATCACTATTCGGTAAATTTTTATAATTCAGCAATTATTAGAGTATATTGTACTCCAATAAT
TGCTCAAAAAGGTGATATAA

Downstream 100 bases:

>100_bases
AATTATAAAATTTATATTTTCTTTAAAATTTAATAAAATTAATATGAATAAAATTTTAAAAATATTAAATATATTTAAAA
ATTTTTATTTAATTTATTAC

Product: hypothetical protein

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MIKPIIVGNWKLNGSKKLIESFLKQLNQFLIKYYKICTVVIAPPVLYSHLIQNMFSSDKKNFFLGAQNIDIHFSGPFTGE
ISPIMLKDIGIKYVIIGHSERRLNHKETNEIIAKKFCMLKEENLVPILCIGETKKEKIDKKTKDICKKQIDIIFNMYGSN
AFDNSIIAYEPIWSIGSGNSAAPKEVQCIAHFIRNYVKSKSNHNIENFFIQYGGSVTKNNAKELIYQKDIDGFLVGGASL
QLEEFSKIIEITNI

Sequences:

>Translated_254_residues
MIKPIIVGNWKLNGSKKLIESFLKQLNQFLIKYYKICTVVIAPPVLYSHLIQNMFSSDKKNFFLGAQNIDIHFSGPFTGE
ISPIMLKDIGIKYVIIGHSERRLNHKETNEIIAKKFCMLKEENLVPILCIGETKKEKIDKKTKDICKKQIDIIFNMYGSN
AFDNSIIAYEPIWSIGSGNSAAPKEVQCIAHFIRNYVKSKSNHNIENFFIQYGGSVTKNNAKELIYQKDIDGFLVGGASL
QLEEFSKIIEITNI
>Mature_254_residues
MIKPIIVGNWKLNGSKKLIESFLKQLNQFLIKYYKICTVVIAPPVLYSHLIQNMFSSDKKNFFLGAQNIDIHFSGPFTGE
ISPIMLKDIGIKYVIIGHSERRLNHKETNEIIAKKFCMLKEENLVPILCIGETKKEKIDKKTKDICKKQIDIIFNMYGSN
AFDNSIIAYEPIWSIGSGNSAAPKEVQCIAHFIRNYVKSKSNHNIENFFIQYGGSVTKNNAKELIYQKDIDGFLVGGASL
QLEEFSKIIEITNI

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=247, Percent_Identity=40.080971659919, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI226529917, Length=247, Percent_Identity=40.080971659919, Blast_Score=158, Evalue=4e-39,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=48.8, Blast_Score=265, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17536593, Length=243, Percent_Identity=39.5061728395062, Blast_Score=165, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320255, Length=247, Percent_Identity=40.4858299595142, Blast_Score=159, Evalue=5e-40,
Organism=Drosophila melanogaster, GI28572008, Length=247, Percent_Identity=41.2955465587045, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI28572006, Length=247, Percent_Identity=41.2955465587045, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=41.2955465587045, Blast_Score=170, Evalue=1e-42,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_BUCCC (Q057N9)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802753.1
- ProteinModelPortal:   Q057N9
- SMR:   Q057N9
- STRING:   Q057N9
- EnsemblBacteria:   EBBUCT00000001553
- GeneID:   4440682
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_188
- eggNOG:   COG0149
- GeneTree:   EBGT00050000007988
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- BioCyc:   BAPH372461:BCC_188-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 28916; Mature: 28916

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 98-98 ACT_SITE 170-170 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPIIVGNWKLNGSKKLIESFLKQLNQFLIKYYKICTVVIAPPVLYSHLIQNMFSSDKK
CCCCEEEECEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NFFLGAQNIDIHFSGPFTGEISPIMLKDIGIKYVIIGHSERRLNHKETNEIIAKKFCMLK
CEEEECCEEEEEECCCCCCCCCCEEEECCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHC
EENLVPILCIGETKKEKIDKKTKDICKKQIDIIFNMYGSNAFDNSIIAYEPIWSIGSGNS
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEECCCCCC
AAPKEVQCIAHFIRNYVKSKSNHNIENFFIQYGGSVTKNNAKELIYQKDIDGFLVGGASL
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCCHHHHHHHHCCCCEEEECCCCE
QLEEFSKIIEITNI
EHHHHHHHHHHCCC
>Mature Secondary Structure
MIKPIIVGNWKLNGSKKLIESFLKQLNQFLIKYYKICTVVIAPPVLYSHLIQNMFSSDKK
CCCCEEEECEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NFFLGAQNIDIHFSGPFTGEISPIMLKDIGIKYVIIGHSERRLNHKETNEIIAKKFCMLK
CEEEECCEEEEEECCCCCCCCCCEEEECCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHC
EENLVPILCIGETKKEKIDKKTKDICKKQIDIIFNMYGSNAFDNSIIAYEPIWSIGSGNS
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEECCCCCC
AAPKEVQCIAHFIRNYVKSKSNHNIENFFIQYGGSVTKNNAKELIYQKDIDGFLVGGASL
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCCHHHHHHHHCCCCEEEECCCCE
QLEEFSKIIEITNI
EHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA