Click here to switch to the map view.

The map label for this gene is fabI [H]

Identifier: 116515103

GI number: 116515103

Start: 194317

End: 195081

Strand: Direct

Name: fabI [H]

Synonym: BCc_167

Alternate gene names: 116515103

Gene position: 194317-195081 (Clockwise)

Preceding gene: 116515102

Following gene: 116515104

Centisome position: 46.67

GC content: 20.78

Gene sequence:

>765_bases
ATGGCTTTTTTAAAAAAAAAAAAAATTTTAATTTTTGGTATAAAAAATCATTTTTCTATTGCCTGGGGAATAGCACTAGC
AATGTATAAACAAAATGCTAAATTAGCATTTGTATATCACAGTAAAAAATCAAAAAAAAATATTAAAATTTTAGCAAAAA
AAGTAAAATCTAAAATTATTTTATATTGTGATATTAATTCAGACGATTCAATACAAACATTATTTAAAATTTTATTTAAA
AAATGGGGTATGTTTGATGGAATTGTACATTCAATAGCAAATATTTCTAAAATTCAATTAACTAATGATTATATTAAAGT
AATTAATCGTTTAGATTTTTTATATGCTCATGAAGTTAATTCTTTTAGTTTAGTAGCAATTATAAAAGCTTCTAAAAATA
TTTTAAATAAAAATTCTTCTATTTTAACTTTATCATATATAGGTGCTATAAAATATATCCCTTATTATAATATTATGGGT
ATTCCTAAAGCATCGTTAGAATCTAATGTACGATATTTAGCAGTTTCTTTAGGAAAGAAAAATATTAAGATCAATGCTAT
TTCTTCTGGTCCTATTAAAACAGTATCATCATATGGTATCAAAAATTTTAGCAAAATTTTTGATATTTCTAAAAATAATT
CTTTATTTAATAAAAATGTTACTATTAAAGAAATTGGAAATGTAGCAGCATTTTTGTGCTCAAATTTATCACTTGGAATT
ACAGGACAAGTAATATATGTAGATTATGGGTTAAATATTATTTAA

Upstream 100 bases:

>100_bases
TTTAATATTTTTTAAAATTTTTTATTTATATTAGAAATATTTTTTTAGTTTTAATATATTATTTTTTTAAAAAAATAATA
TTTTATAGGTTAATTTGTTT

Downstream 100 bases:

>100_bases
ATAATATTTAATACATTTCATATATATTAAATAAGTAATAAATATATTACTATGATAATTATTATTATTAATTATAATTA
TATTTTTTTATATTTTTAAA

Product: FabI

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MAFLKKKKILIFGIKNHFSIAWGIALAMYKQNAKLAFVYHSKKSKKNIKILAKKVKSKIILYCDINSDDSIQTLFKILFK
KWGMFDGIVHSIANISKIQLTNDYIKVINRLDFLYAHEVNSFSLVAIIKASKNILNKNSSILTLSYIGAIKYIPYYNIMG
IPKASLESNVRYLAVSLGKKNIKINAISSGPIKTVSSYGIKNFSKIFDISKNNSLFNKNVTIKEIGNVAAFLCSNLSLGI
TGQVIYVDYGLNII

Sequences:

>Translated_254_residues
MAFLKKKKILIFGIKNHFSIAWGIALAMYKQNAKLAFVYHSKKSKKNIKILAKKVKSKIILYCDINSDDSIQTLFKILFK
KWGMFDGIVHSIANISKIQLTNDYIKVINRLDFLYAHEVNSFSLVAIIKASKNILNKNSSILTLSYIGAIKYIPYYNIMG
IPKASLESNVRYLAVSLGKKNIKINAISSGPIKTVSSYGIKNFSKIFDISKNNSLFNKNVTIKEIGNVAAFLCSNLSLGI
TGQVIYVDYGLNII
>Mature_253_residues
AFLKKKKILIFGIKNHFSIAWGIALAMYKQNAKLAFVYHSKKSKKNIKILAKKVKSKIILYCDINSDDSIQTLFKILFKK
WGMFDGIVHSIANISKIQLTNDYIKVINRLDFLYAHEVNSFSLVAIIKASKNILNKNSSILTLSYIGAIKYIPYYNIMGI
PKASLESNVRYLAVSLGKKNIKINAISSGPIKTVSSYGIKNFSKIFDISKNNSLFNKNVTIKEIGNVAAFLCSNLSLGIT
GQVIYVDYGLNII

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=253, Percent_Identity=46.6403162055336, Blast_Score=261, Evalue=4e-71,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28478; Mature: 28347

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLKKKKILIFGIKNHFSIAWGIALAMYKQNAKLAFVYHSKKSKKNIKILAKKVKSKII
CCCCCCCEEEEEEECCCCHHHHHHHHEEEECCCEEEEEEECCCCCCHHHHHHHHHCCEEE
LYCDINSDDSIQTLFKILFKKWGMFDGIVHSIANISKIQLTNDYIKVINRLDFLYAHEVN
EEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHEEECCC
SFSLVAIIKASKNILNKNSSILTLSYIGAIKYIPYYNIMGIPKASLESNVRYLAVSLGKK
CEEEEEEEEHHHHHHCCCCCEEEEEEHHHHHCCCCEEECCCCCHHHHCCEEEEEEEECCC
NIKINAISSGPIKTVSSYGIKNFSKIFDISKNNSLFNKNVTIKEIGNVAAFLCSNLSLGI
EEEEEEECCCCCHHHHHCCCCHHHHHEECCCCCCEECCCCCHHHHHHHHHHHHCCCCCCC
TGQVIYVDYGLNII
CEEEEEEECCCEEC
>Mature Secondary Structure 
AFLKKKKILIFGIKNHFSIAWGIALAMYKQNAKLAFVYHSKKSKKNIKILAKKVKSKII
CCCCCCEEEEEEECCCCHHHHHHHHEEEECCCEEEEEEECCCCCCHHHHHHHHHCCEEE
LYCDINSDDSIQTLFKILFKKWGMFDGIVHSIANISKIQLTNDYIKVINRLDFLYAHEVN
EEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHEEECCC
SFSLVAIIKASKNILNKNSSILTLSYIGAIKYIPYYNIMGIPKASLESNVRYLAVSLGKK
CEEEEEEEEHHHHHHCCCCCEEEEEEHHHHHCCCCEEECCCCCHHHHCCEEEEEEEECCC
NIKINAISSGPIKTVSSYGIKNFSKIFDISKNNSLFNKNVTIKEIGNVAAFLCSNLSLGI
EEEEEEECCCCCHHHHHCCCCHHHHHEECCCCCCEECCCCCHHHHHHHHHHHHCCCCCCC
TGQVIYVDYGLNII
CEEEEEEECCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]