Click here to switch to the map view.

The map label for this gene is tsf

Identifier: 116515083

GI number: 116515083

Start: 168112

End: 168942

Strand: Direct

Name: tsf

Synonym: BCc_143

Alternate gene names: 116515083

Gene position: 168112-168942 (Clockwise)

Preceding gene: 116515082

Following gene: 116515084

Centisome position: 40.37

GC content: 21.3

Gene sequence:

>831_bases
ATGAAAATTTCTGTAAAACTTATTAAAGAATTGCGTATAAAAACTGGATCAGGATATTTGGAATGTAAAAGAGCTTTACA
AAAATCTAATGGAAATATTATTAACGCAATTAATTATTTAAGAATTGTAGGTACTGATATTGCTCAAAGGAAAGTATTAC
GAAAAACAAAATTTGGTCGTATTTTTTCATATTGTAGTAAAAATTTAGGTGTTTTATTAGAATTAACTTCAGAAACAGAT
TTTGTTTCAAAAAATGAAGAATTTAAAAATTTTGGAGAAAAAATTGTTAATTTTTCTGGTAATAATAAAATTTTTGATTT
AACAGAAATTAATGAAATTTTTAATTCAAAAAAAATAAATTTTATTTCTAGAGTTCGTGAAAATATAGAAATTAATAAAA
TTAAGTATATTACTGGAAATATTATAGAATCATATCAACATTTAGGTAAAATTGGAGTAATTATTTCTGGAAAAATGTTA
TCACCTAATACTCACTTAAATACAACTAAATGTTTTAAAAATATTGCTATGCATGTAGCAGCTGCTGCTCCATTATATTT
ATCAGAATTAGATATTCCTAATAACGTTCTTCAAAAAGAAACTGATATTCAAAAGAGTATAGCTAAAAAAACTGGTAAAA
GTTCAAAAATATTACAAGCAATTATTAAGGGTCGTTTAAAAAAATTTATTAGTGAAATTACTTTGATTAATCAAAATTTT
ATTATAAATCCTAAAATTACTATACATGATTATTTAAAAGAAAATCAAGTTTGGATAAATAATTTTATACGTTTACAAGT
AGGAGAAAATATTGATAATCTTAATACATAA

Upstream 100 bases:

>100_bases
AAAAAGAATCAATTATTTTAAAAAATAGATTAAAAGAACAATAATTTTAAAATTTTTCTATATTATCAATTAATAATATT
AATATTAGGTAAGAAATAAT

Downstream 100 bases:

>100_bases
TTAATAATATTAATATTTAAGAAAATATAATTATTTTTTATATTTTTTTAAATAAATTTTTTATATAGTTATGTACATTT
GATATATTTTGATATTTATG

Product: Tsf

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKISVKLIKELRIKTGSGYLECKRALQKSNGNIINAINYLRIVGTDIAQRKVLRKTKFGRIFSYCSKNLGVLLELTSETD
FVSKNEEFKNFGEKIVNFSGNNKIFDLTEINEIFNSKKINFISRVRENIEINKIKYITGNIIESYQHLGKIGVIISGKML
SPNTHLNTTKCFKNIAMHVAAAAPLYLSELDIPNNVLQKETDIQKSIAKKTGKSSKILQAIIKGRLKKFISEITLINQNF
IINPKITIHDYLKENQVWINNFIRLQVGENIDNLNT

Sequences:

>Translated_276_residues
MKISVKLIKELRIKTGSGYLECKRALQKSNGNIINAINYLRIVGTDIAQRKVLRKTKFGRIFSYCSKNLGVLLELTSETD
FVSKNEEFKNFGEKIVNFSGNNKIFDLTEINEIFNSKKINFISRVRENIEINKIKYITGNIIESYQHLGKIGVIISGKML
SPNTHLNTTKCFKNIAMHVAAAAPLYLSELDIPNNVLQKETDIQKSIAKKTGKSSKILQAIIKGRLKKFISEITLINQNF
IINPKITIHDYLKENQVWINNFIRLQVGENIDNLNT
>Mature_276_residues
MKISVKLIKELRIKTGSGYLECKRALQKSNGNIINAINYLRIVGTDIAQRKVLRKTKFGRIFSYCSKNLGVLLELTSETD
FVSKNEEFKNFGEKIVNFSGNNKIFDLTEINEIFNSKKINFISRVRENIEINKIKYITGNIIESYQHLGKIGVIISGKML
SPNTHLNTTKCFKNIAMHVAAAAPLYLSELDIPNNVLQKETDIQKSIAKKTGKSSKILQAIIKGRLKKFISEITLINQNF
IINPKITIHDYLKENQVWINNFIRLQVGENIDNLNT

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Escherichia coli, GI1786366, Length=275, Percent_Identity=34.1818181818182, Blast_Score=194, Evalue=7e-51,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_BUCCC (Q057T0)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802712.1
- ProteinModelPortal:   Q057T0
- SMR:   Q057T0
- STRING:   Q057T0
- EnsemblBacteria:   EBBUCT00000001217
- GeneID:   4440884
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_143
- eggNOG:   COG0264
- GeneTree:   EBGT00050000007971
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- BioCyc:   BAPH372461:BCC_143-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 31464; Mature: 31464

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISVKLIKELRIKTGSGYLECKRALQKSNGNIINAINYLRIVGTDIAQRKVLRKTKFGR
CEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHH
IFSYCSKNLGVLLELTSETDFVSKNEEFKNFGEKIVNFSGNNKIFDLTEINEIFNSKKIN
HHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHEECCCCCEEEEHHHHHHHHCCCCHH
FISRVRENIEINKIKYITGNIIESYQHLGKIGVIISGKMLSPNTHLNTTKCFKNIAMHVA
HHHHHHHCCCEEEEEEEHHHHHHHHHHHCCEEEEEECEEECCCCCCCHHHHHHHHHHHHH
AAAPLYLSELDIPNNVLQKETDIQKSIAKKTGKSSKILQAIIKGRLKKFISEITLINQNF
HHCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
IINPKITIHDYLKENQVWINNFIRLQVGENIDNLNT
EECCCEEHHHHHHCCCEEEEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKISVKLIKELRIKTGSGYLECKRALQKSNGNIINAINYLRIVGTDIAQRKVLRKTKFGR
CEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHH
IFSYCSKNLGVLLELTSETDFVSKNEEFKNFGEKIVNFSGNNKIFDLTEINEIFNSKKIN
HHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHEECCCCCEEEEHHHHHHHHCCCCHH
FISRVRENIEINKIKYITGNIIESYQHLGKIGVIISGKMLSPNTHLNTTKCFKNIAMHVA
HHHHHHHCCCEEEEEEEHHHHHHHHHHHCCEEEEEECEEECCCCCCCHHHHHHHHHHHHH
AAAPLYLSELDIPNNVLQKETDIQKSIAKKTGKSSKILQAIIKGRLKKFISEITLINQNF
HHCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
IINPKITIHDYLKENQVWINNFIRLQVGENIDNLNT
EECCCEEHHHHHHCCCEEEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA