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The map label for this gene is pcnB [H]

Identifier: 116515068

GI number: 116515068

Start: 146438

End: 147661

Strand: Reverse

Name: pcnB [H]

Synonym: BCc_128

Alternate gene names: 116515068

Gene position: 147661-146438 (Counterclockwise)

Preceding gene: 116515074

Following gene: 116515067

Centisome position: 35.46

GC content: 18.71

Gene sequence:

>1224_bases
ATGAAGCTAATTATAAATAAAAATCATAACATTTCTAAAAAAAAAATTAGTAGAAATTCTATGAAAGTATTATATAGATT
AAATAAATTAGGCTATGAAGCATATTTAGTGGGAGGAGGGGTAAGAGATTTATTATTAGGAAAAAAACCTAAAGATTTTG
ATATTGCTACTAACGCAAAACCTGATGAAATCAGAAAACTATTTAAAAATTGCCGATTAATAGGAAGAAGATTTATAATT
GCGCATTTAATATTCAAAAATGAAATTATTGAAGTATCAACATTTCGAGCAAAAAATAATCATTTGAAAAAAAATAATTT
ATATTACAAAAAAATAAAAACAAAAAAAGGAATATTATTATCAGATAATACATTTGGAAAAATTGAAGAAGATGCTTATA
GACGTGATATAACAATAAATGCATTATATTATAGTATAAAAGATTTTGGAATTAGAGACTATATAGGAGGAATAAAAGAT
ATAAAATTAAAAAAAATACGTTTAATTGGAAATGCTGAAACACGATATAGAGAAGATCCTGTACGAATGTTAAGAGTAAT
ACGATTTTCAGTACAATTGAATATGCATATTGATAAAAAAACTGAAAAACCGATAATAAAATTATCAAATTTATTAAAAG
AAATACCATCAGCAAGATTATTTAATGAATCAATAAAATTATTTTGTTTTGGATATGGTTATTTAACTTATATACGATTA
AGAAAATATTCATTAATTTATCCATTATTACCATTTTTATTTAATCAGTTAAGTCAAAAAAACACATTTTTTTTAAAAAA
AATTACAATAAATTGTTTAAAAAAAATAGATAAAAAAGGAAAAAATAAAAAAATCAGTTGTCCATCTTTTTTATTTGCAT
CATTATTATGGTATCTTTTAATTGAAAAAACACAAATATTATCAATTAAAAAAAAAATAAAATTTTCTAAAGCATTTTTA
ATTTCTATTAATTATATTTTAAAAAAAAATTCAATATCAATAGGTATACCTAAAAATATTTTATTAATAATCAAAAAAAT
ATGGAAATTGCAAAAAGATATTGAAAAAAGAAAAAAAAACACAATTAAAAAAATAATAAATCATAATAATTTTATTCAAG
CTATAGAATTATTTTCATTACGAGTTGAAATCGAAAATCATATTAAATTAAAAAAAATCTTTATTTTTTGGAATAAACAA
AATTTTTTTTTAAAAAAAAAATAA

Upstream 100 bases:

>100_bases
TATATATTTATAAATAATAAATAAAAAAAATTTTTTTATTTAACAACATAGCATTAGTTAACTAAAAATTAAAAACATAA
AAAAAAATCGATATTCGCAT

Downstream 100 bases:

>100_bases
TTTACTAAAAATAAAAAACATAAAACAACATACGTTTCTTTAAAATACTTATTTTTTTTAAAATTTATTTTTTAAAAAAT
AATTATACCTCCTTATCAAA

Product: hypothetical protein

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKLIINKNHNISKKKISRNSMKVLYRLNKLGYEAYLVGGGVRDLLLGKKPKDFDIATNAKPDEIRKLFKNCRLIGRRFII
AHLIFKNEIIEVSTFRAKNNHLKKNNLYYKKIKTKKGILLSDNTFGKIEEDAYRRDITINALYYSIKDFGIRDYIGGIKD
IKLKKIRLIGNAETRYREDPVRMLRVIRFSVQLNMHIDKKTEKPIIKLSNLLKEIPSARLFNESIKLFCFGYGYLTYIRL
RKYSLIYPLLPFLFNQLSQKNTFFLKKITINCLKKIDKKGKNKKISCPSFLFASLLWYLLIEKTQILSIKKKIKFSKAFL
ISINYILKKNSISIGIPKNILLIIKKIWKLQKDIEKRKKNTIKKIINHNNFIQAIELFSLRVEIENHIKLKKIFIFWNKQ
NFFLKKK

Sequences:

>Translated_407_residues
MKLIINKNHNISKKKISRNSMKVLYRLNKLGYEAYLVGGGVRDLLLGKKPKDFDIATNAKPDEIRKLFKNCRLIGRRFII
AHLIFKNEIIEVSTFRAKNNHLKKNNLYYKKIKTKKGILLSDNTFGKIEEDAYRRDITINALYYSIKDFGIRDYIGGIKD
IKLKKIRLIGNAETRYREDPVRMLRVIRFSVQLNMHIDKKTEKPIIKLSNLLKEIPSARLFNESIKLFCFGYGYLTYIRL
RKYSLIYPLLPFLFNQLSQKNTFFLKKITINCLKKIDKKGKNKKISCPSFLFASLLWYLLIEKTQILSIKKKIKFSKAFL
ISINYILKKNSISIGIPKNILLIIKKIWKLQKDIEKRKKNTIKKIINHNNFIQAIELFSLRVEIENHIKLKKIFIFWNKQ
NFFLKKK
>Mature_407_residues
MKLIINKNHNISKKKISRNSMKVLYRLNKLGYEAYLVGGGVRDLLLGKKPKDFDIATNAKPDEIRKLFKNCRLIGRRFII
AHLIFKNEIIEVSTFRAKNNHLKKNNLYYKKIKTKKGILLSDNTFGKIEEDAYRRDITINALYYSIKDFGIRDYIGGIKD
IKLKKIRLIGNAETRYREDPVRMLRVIRFSVQLNMHIDKKTEKPIIKLSNLLKEIPSARLFNESIKLFCFGYGYLTYIRL
RKYSLIYPLLPFLFNQLSQKNTFFLKKITINCLKKIDKKGKNKKISCPSFLFASLLWYLLIEKTQILSIKKKIKFSKAFL
ISINYILKKNSISIGIPKNILLIIKKIWKLQKDIEKRKKNTIKKIINHNNFIQAIELFSLRVEIENHIKLKKIFIFWNKQ
NFFLKKK

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=174, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI87081691, Length=399, Percent_Identity=47.3684210526316, Blast_Score=404, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321016, Length=189, Percent_Identity=31.2169312169312, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281360112, Length=249, Percent_Identity=28.9156626506024, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357337, Length=249, Percent_Identity=28.9156626506024, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 48234; Mature: 48234

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIINKNHNISKKKISRNSMKVLYRLNKLGYEAYLVGGGVRDLLLGKKPKDFDIATNAK
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCCCCCCCC
PDEIRKLFKNCRLIGRRFIIAHLIFKNEIIEVSTFRAKNNHLKKNNLYYKKIKTKKGILL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCEEEEEECCCCCEEE
SDNTFGKIEEDAYRRDITINALYYSIKDFGIRDYIGGIKDIKLKKIRLIGNAETRYREDP
ECCCCCCCCHHHHHCCEEEEEEEEEHHHCCHHHHHCCCCCCEEEEEEEECCCCHHHCCCH
VRMLRVIRFSVQLNMHIDKKTEKPIIKLSNLLKEIPSARLFNESIKLFCFGYGYLTYIRL
HHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHCCCEEEEEEEHHHHHHHHH
RKYSLIYPLLPFLFNQLSQKNTFFLKKITINCLKKIDKKGKNKKISCPSFLFASLLWYLL
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
IEKTQILSIKKKIKFSKAFLISINYILKKNSISIGIPKNILLIIKKIWKLQKDIEKRKKN
HHHHHHHHHHHHHHHCEEEEEEEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
TIKKIINHNNFIQAIELFSLRVEIENHIKLKKIFIFWNKQNFFLKKK
HHHHHHCCCCHHHHHHHHHEEEEECCCEEEEEEEEEECCCCEEEECC
>Mature Secondary Structure
MKLIINKNHNISKKKISRNSMKVLYRLNKLGYEAYLVGGGVRDLLLGKKPKDFDIATNAK
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCCCCCCCC
PDEIRKLFKNCRLIGRRFIIAHLIFKNEIIEVSTFRAKNNHLKKNNLYYKKIKTKKGILL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCEEEEEECCCCCEEE
SDNTFGKIEEDAYRRDITINALYYSIKDFGIRDYIGGIKDIKLKKIRLIGNAETRYREDP
ECCCCCCCCHHHHHCCEEEEEEEEEHHHCCHHHHHCCCCCCEEEEEEEECCCCHHHCCCH
VRMLRVIRFSVQLNMHIDKKTEKPIIKLSNLLKEIPSARLFNESIKLFCFGYGYLTYIRL
HHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHCCCEEEEEEEHHHHHHHHH
RKYSLIYPLLPFLFNQLSQKNTFFLKKITINCLKKIDKKGKNKKISCPSFLFASLLWYLL
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
IEKTQILSIKKKIKFSKAFLISINYILKKNSISIGIPKNILLIIKKIWKLQKDIEKRKKN
HHHHHHHHHHHHHHHCEEEEEEEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
TIKKIINHNNFIQAIELFSLRVEIENHIKLKKIFIFWNKQNFFLKKK
HHHHHHCCCCHHHHHHHHHEEEEECCCEEEEEEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]