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The map label for this gene is glyS [H]

Identifier: 116515026

GI number: 116515026

Start: 92883

End: 94823

Strand: Reverse

Name: glyS [H]

Synonym: BCc_085

Alternate gene names: 116515026

Gene position: 94823-92883 (Counterclockwise)

Preceding gene: 116515027

Following gene: 116515017

Centisome position: 22.77

GC content: 15.87

Gene sequence:

>1941_bases
ATGAATCAAAAAATATTATTAATCGAAATACAAACAGAAGATTTACCTAGTAAAGAATTAAAAAACATAGCAAATTTTTT
TTATTATATTTTTAAAAAAAAAATCAAAGAAAATAAAATTCAATACAAAAAAATTTCTTTATTTTTTACCTCTAAAAAAT
TAGTATTAAAGGTCTTTTCTTTAAAAAACATAAAAAAAAAAAAAAATCATCTAATTTATATACATAAAAAAAAAACTATT
TCTTTAAAAGAAAAAATAAAAAAAATAGTATTTGAAACAATTAAAAAAATACCATTAAAAAGATCTATGTTGTGGGATGA
AAAATCTATTCGATTTTCTAGACCCATACGTAATATAATGATTTTACTAAACAAAAAAATCATTAAAGCTAAAATTGAAG
GAATTACTTCTAATAGTATTTCTTATGGACATTTTTTAATGTCTCCTAAAAAAATTATTTTTCATCATGCAAATCAATAT
ACAAAAAAATTTTTTAAGAAAAAAAAAATTATATTAGATTATCAAGAAAGAAAAAAAAAAATAATAAAACAAATAAAAAA
TCTAACAAAAAAACATAATAAAAAAATTTATATAAATAAAAATTTGTTAAAAGAAATAAGTGTATCAATCGAATGGCCTA
TTGCTCATATAGGAAAATTTAAAAAAAAATATTTAAATATACCTCAGGAAATATTAATATATATTATTGAAAATAATCAA
AAATATTTTTCTATCTTTAATACTAAGAAGAATACAATTACAAATTACTTTATTTTTATTAGTAACATTGAAACCCCAAA
TTTTAAAAAAATTATTCAAGAAAATGAACAAGTATTATCTTCTAAACTAAAAAATATAAATTTTTTTTTAAAAAAGGATT
TACTTATAAAGTTTTCTAACAGATTAGATTTATTAAAAAATATTTCTTTTCAAAAAAAACTAGGATCTATGTACGAAAAA
ACAATACGAATAAAAAAAATCAGTCAATATATTTCAAAAATTCTAAAAGTAAAAAAAAAATCAATTAAACGTGCTGCTAT
TTTATCAAAATGTGATTTAACAACAAATATAATACGAAGTACAACGCTTCAAGGTATTATTGGTATGTATTATGCTTTAT
ATAATCATGAAGATAGAGAAATAGCTTTAGCTATAAAAGAACATTATTTACCTAATTTTTCTCAATCTAATTTACCTACT
CAATTAATATCTTGTATATTAAGTATTTCTGATAAAATCGATACTATAACTGGAATTTTTAGTATTAGTAAAGTACCTAA
AAATAATAAAGATCCATTTGCATTACGTAGATCTACTATAGGTATTATTAAAATAATTATTAAAAAAAAAATATATATTG
ATATTATTAAATTAATTAAATATTCAATAAATTTATATACACATATAAAAAATAAAATATTAATTCAAAAATTAATTTTA
AATTTTATATATTCAAAATTTCAATCATTATATTTAAAAAAAAAATATAATAAAAAAATTATTTCTTCTGTTCTTTCACT
GAAATTAAATGATTTATTAGATATTAATAATCGCATACACGCATTATATAATTTTCAAAAAATTAATAATATGAAAAATA
TACTTAATATTTATAAAAGAATAACGAATATATTAAAACAAAAAAAAGAAAAAAAAACAAAAATAAACATTTTTTATTCA
AATCCTATATATAAATTTTCAATATATGAAAAAAAAATAATTATATTAATAAAATCTATTATAGAAAATAATATAAAAAA
AGAAAAAAATGATTATTTTAATTTATTGAAAAAAATTGAAGAATTATGTACACCAATAAAAGAATTTTTCCAAAATATAT
TAATAAATGATAAAAATACTGAAAAACGTTTGTATCGTATATCTATATTACAAGAAATCAAAAAAATATTTTTATATATA
ACAAACTTTTCTTATTTATAA

Upstream 100 bases:

>100_bases
GTTATCTACTACTGAACGAACAAATTATATTCTATGTATACGGAAAATAATCAAAAAAATTGCTGAAAAATATATTAATA
TAAACGTTAAAGAGTAAAAA

Downstream 100 bases:

>100_bases
ATTAATAAAATAAATATTATAAATACTTTTATTTTTAAATAATTTTAAATATTTTTAATTAAATTAAATATTTTCTATAA
ATATTAAAATCTTTATAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MNQKILLIEIQTEDLPSKELKNIANFFYYIFKKKIKENKIQYKKISLFFTSKKLVLKVFSLKNIKKKKNHLIYIHKKKTI
SLKEKIKKIVFETIKKIPLKRSMLWDEKSIRFSRPIRNIMILLNKKIIKAKIEGITSNSISYGHFLMSPKKIIFHHANQY
TKKFFKKKKIILDYQERKKKIIKQIKNLTKKHNKKIYINKNLLKEISVSIEWPIAHIGKFKKKYLNIPQEILIYIIENNQ
KYFSIFNTKKNTITNYFIFISNIETPNFKKIIQENEQVLSSKLKNINFFLKKDLLIKFSNRLDLLKNISFQKKLGSMYEK
TIRIKKISQYISKILKVKKKSIKRAAILSKCDLTTNIIRSTTLQGIIGMYYALYNHEDREIALAIKEHYLPNFSQSNLPT
QLISCILSISDKIDTITGIFSISKVPKNNKDPFALRRSTIGIIKIIIKKKIYIDIIKLIKYSINLYTHIKNKILIQKLIL
NFIYSKFQSLYLKKKYNKKIISSVLSLKLNDLLDINNRIHALYNFQKINNMKNILNIYKRITNILKQKKEKKTKINIFYS
NPIYKFSIYEKKIIILIKSIIENNIKKEKNDYFNLLKKIEELCTPIKEFFQNILINDKNTEKRLYRISILQEIKKIFLYI
TNFSYL

Sequences:

>Translated_646_residues
MNQKILLIEIQTEDLPSKELKNIANFFYYIFKKKIKENKIQYKKISLFFTSKKLVLKVFSLKNIKKKKNHLIYIHKKKTI
SLKEKIKKIVFETIKKIPLKRSMLWDEKSIRFSRPIRNIMILLNKKIIKAKIEGITSNSISYGHFLMSPKKIIFHHANQY
TKKFFKKKKIILDYQERKKKIIKQIKNLTKKHNKKIYINKNLLKEISVSIEWPIAHIGKFKKKYLNIPQEILIYIIENNQ
KYFSIFNTKKNTITNYFIFISNIETPNFKKIIQENEQVLSSKLKNINFFLKKDLLIKFSNRLDLLKNISFQKKLGSMYEK
TIRIKKISQYISKILKVKKKSIKRAAILSKCDLTTNIIRSTTLQGIIGMYYALYNHEDREIALAIKEHYLPNFSQSNLPT
QLISCILSISDKIDTITGIFSISKVPKNNKDPFALRRSTIGIIKIIIKKKIYIDIIKLIKYSINLYTHIKNKILIQKLIL
NFIYSKFQSLYLKKKYNKKIISSVLSLKLNDLLDINNRIHALYNFQKINNMKNILNIYKRITNILKQKKEKKTKINIFYS
NPIYKFSIYEKKIIILIKSIIENNIKKEKNDYFNLLKKIEELCTPIKEFFQNILINDKNTEKRLYRISILQEIKKIFLYI
TNFSYL
>Mature_646_residues
MNQKILLIEIQTEDLPSKELKNIANFFYYIFKKKIKENKIQYKKISLFFTSKKLVLKVFSLKNIKKKKNHLIYIHKKKTI
SLKEKIKKIVFETIKKIPLKRSMLWDEKSIRFSRPIRNIMILLNKKIIKAKIEGITSNSISYGHFLMSPKKIIFHHANQY
TKKFFKKKKIILDYQERKKKIIKQIKNLTKKHNKKIYINKNLLKEISVSIEWPIAHIGKFKKKYLNIPQEILIYIIENNQ
KYFSIFNTKKNTITNYFIFISNIETPNFKKIIQENEQVLSSKLKNINFFLKKDLLIKFSNRLDLLKNISFQKKLGSMYEK
TIRIKKISQYISKILKVKKKSIKRAAILSKCDLTTNIIRSTTLQGIIGMYYALYNHEDREIALAIKEHYLPNFSQSNLPT
QLISCILSISDKIDTITGIFSISKVPKNNKDPFALRRSTIGIIKIIIKKKIYIDIIKLIKYSINLYTHIKNKILIQKLIL
NFIYSKFQSLYLKKKYNKKIISSVLSLKLNDLLDINNRIHALYNFQKINNMKNILNIYKRITNILKQKKEKKTKINIFYS
NPIYKFSIYEKKIIILIKSIIENNIKKEKNDYFNLLKKIEELCTPIKEFFQNILINDKNTEKRLYRISILQEIKKIFLYI
TNFSYL

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=697, Percent_Identity=28.4074605451937, Blast_Score=385, Evalue=1e-108,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 77142; Mature: 77142

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKILLIEIQTEDLPSKELKNIANFFYYIFKKKIKENKIQYKKISLFFTSKKLVLKVFS
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECHHHHHHHHH
LKNIKKKKNHLIYIHKKKTISLKEKIKKIVFETIKKIPLKRSMLWDEKSIRFSRPIRNIM
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHH
ILLNKKIIKAKIEGITSNSISYGHFLMSPKKIIFHHANQYTKKFFKKKKIILDYQERKKK
HHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHEECHHHHHHH
IIKQIKNLTKKHNKKIYINKNLLKEISVSIEWPIAHIGKFKKKYLNIPQEILIYIIENNQ
HHHHHHHHHHHCCCEEEEEHHHHHHHHCEEECCHHHHHHHHHHHHCCCHHHEEEEEECCC
KYFSIFNTKKNTITNYFIFISNIETPNFKKIIQENEQVLSSKLKNINFFLKKDLLIKFSN
EEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
RLDLLKNISFQKKLGSMYEKTIRIKKISQYISKILKVKKKSIKRAAILSKCDLTTNIIRS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TTLQGIIGMYYALYNHEDREIALAIKEHYLPNFSQSNLPTQLISCILSISDKIDTITGIF
HHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SISKVPKNNKDPFALRRSTIGIIKIIIKKKIYIDIIKLIKYSINLYTHIKNKILIQKLIL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFIYSKFQSLYLKKKYNKKIISSVLSLKLNDLLDINNRIHALYNFQKINNMKNILNIYKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
ITNILKQKKEKKTKINIFYSNPIYKFSIYEKKIIILIKSIIENNIKKEKNDYFNLLKKIE
HHHHHHHHHCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELCTPIKEFFQNILINDKNTEKRLYRISILQEIKKIFLYITNFSYL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNQKILLIEIQTEDLPSKELKNIANFFYYIFKKKIKENKIQYKKISLFFTSKKLVLKVFS
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECHHHHHHHHH
LKNIKKKKNHLIYIHKKKTISLKEKIKKIVFETIKKIPLKRSMLWDEKSIRFSRPIRNIM
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHH
ILLNKKIIKAKIEGITSNSISYGHFLMSPKKIIFHHANQYTKKFFKKKKIILDYQERKKK
HHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHEECHHHHHHH
IIKQIKNLTKKHNKKIYINKNLLKEISVSIEWPIAHIGKFKKKYLNIPQEILIYIIENNQ
HHHHHHHHHHHCCCEEEEEHHHHHHHHCEEECCHHHHHHHHHHHHCCCHHHEEEEEECCC
KYFSIFNTKKNTITNYFIFISNIETPNFKKIIQENEQVLSSKLKNINFFLKKDLLIKFSN
EEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
RLDLLKNISFQKKLGSMYEKTIRIKKISQYISKILKVKKKSIKRAAILSKCDLTTNIIRS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TTLQGIIGMYYALYNHEDREIALAIKEHYLPNFSQSNLPTQLISCILSISDKIDTITGIF
HHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SISKVPKNNKDPFALRRSTIGIIKIIIKKKIYIDIIKLIKYSINLYTHIKNKILIQKLIL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFIYSKFQSLYLKKKYNKKIISSVLSLKLNDLLDINNRIHALYNFQKINNMKNILNIYKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
ITNILKQKKEKKTKINIFYSNPIYKFSIYEKKIIILIKSIIENNIKKEKNDYFNLLKKIE
HHHHHHHHHCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELCTPIKEFFQNILINDKNTEKRLYRISILQEIKKIFLYITNFSYL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]