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The map label for this gene is mesJ [H]

Identifier: 116515015

GI number: 116515015

Start: 80573

End: 81880

Strand: Reverse

Name: mesJ [H]

Synonym: BCc_073

Alternate gene names: 116515015

Gene position: 81880-80573 (Counterclockwise)

Preceding gene: 116515017

Following gene: 116515003

Centisome position: 19.66

GC content: 16.06

Gene sequence:

>1308_bases
ATGTTTATAAAAAAATTATTTAAAAAATATCCTACAAAAAAAAAATTTTTATTAGCCCTTAGTGGTGGCGTTGATTCTAC
TGTCTTATTCTATCAATTAATTATATGGAAAAAAAAATTTCAAGAAATAAATTTTAGAACTATACATATTAATCATCATT
TACATTCACATGCAAAATTAGCTCAAAAACATTGTATAAATATTTGTAAAAAAAACAAAATTGAGATAAAAATTTTTGAT
ATTATCATTCCAAAAAAAAACAAATATGGAATAGAAGGTTATTCTAGACTAGAAAGATTAAAAATATTTAAAAAAAATAT
ATTACCTGAAGAAATATTATTAACAGGTCATAATATGAATGATCTGTGTGAAAATATTTTTTTATCTCTTAAACGAAAAA
GTGGAATTAACGGAATATCAGGAATGAATTTTTATTCAAAATATGATAATATCAAAATTATACGACCATTAATTAATATA
AAAAAAGAAAATATTTTATTTTGGGCAAAAAAAAAAAAAATCAAATGGATAGAAGATCAATCAAATAAAAATAATCAATA
TGATAGAAATTTTATTAGAAATAAAATTTTATCAAAAATATATCTTAAATGGCCATATTTTTTAAAAAATTCTATTAAAA
GTATTAATTTATTAAAAAAAGATAAAAAAATATTAAATTATTTTTTATCAAAGTTTCTTAAAAAAAATATTTATTTAGAT
AAAACATTATCTTTAATAAATTTTGATAATCTAATATTAGAAGCAAAATTTTCTATTTTAAAAATGTGGTTATATAACAA
TAGTCAAATTATTCCTACATATTATATAATCAAAAGAATATATAATGAATTAATAGATAACGAAAACTATTTTAATAAAA
AAATTCTTTTTCAAAAAAAAGAAATAAGAAGATACAAAAAAAACATTTATTATATAAATCCAGAAAAAAAAGAAAAAAAA
ATTGTTCTATATTGGAAAAATATTTTTCAAAAAATAAAACTGCCAAATAAATTAGGTTATTTAATTGCACATATAAAAAA
AAAAAAAAAAACAAAAAAAAATATTTCTTATATACCTTTTCCTAAAAAAAACACACTTATAACAGTATGTTTTCATATAA
AAAAAAAAAAAACAAAAATAAAAAAAATTTGGCAAAAATATAATATACCACCATGGTTAAGAAATAATATACCATTATTA
TTTTATAATAAAAAATTAATATCATGTATTGGTATATTTATAGAAAAAGAAAAAAAAAAAGAAAAAAAAAAATATATATC
AATTAAATGGATAAACAAAATAAATTAA

Upstream 100 bases:

>100_bases
ATACGTTCGTAACTCAATGGTTAGAGTGTTACTATGACATGGTAAAAGTTAGTGGTTCAAATCCACTCGAACGTATCAAT
ATATCTTATATTTATACATT

Downstream 100 bases:

>100_bases
TTATATTACTTTCTTTAAAAATTTATCTATATCATTAATATTAATACGTTTATAAAAACAAGAAAAATTATTCAGTTTAT
GATTAGTTAGTGGAATTTTA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MFIKKLFKKYPTKKKFLLALSGGVDSTVLFYQLIIWKKKFQEINFRTIHINHHLHSHAKLAQKHCINICKKNKIEIKIFD
IIIPKKNKYGIEGYSRLERLKIFKKNILPEEILLTGHNMNDLCENIFLSLKRKSGINGISGMNFYSKYDNIKIIRPLINI
KKENILFWAKKKKIKWIEDQSNKNNQYDRNFIRNKILSKIYLKWPYFLKNSIKSINLLKKDKKILNYFLSKFLKKNIYLD
KTLSLINFDNLILEAKFSILKMWLYNNSQIIPTYYIIKRIYNELIDNENYFNKKILFQKKEIRRYKKNIYYINPEKKEKK
IVLYWKNIFQKIKLPNKLGYLIAHIKKKKKTKKNISYIPFPKKNTLITVCFHIKKKKTKIKKIWQKYNIPPWLRNNIPLL
FYNKKLISCIGIFIEKEKKKEKKKYISIKWINKIN

Sequences:

>Translated_435_residues
MFIKKLFKKYPTKKKFLLALSGGVDSTVLFYQLIIWKKKFQEINFRTIHINHHLHSHAKLAQKHCINICKKNKIEIKIFD
IIIPKKNKYGIEGYSRLERLKIFKKNILPEEILLTGHNMNDLCENIFLSLKRKSGINGISGMNFYSKYDNIKIIRPLINI
KKENILFWAKKKKIKWIEDQSNKNNQYDRNFIRNKILSKIYLKWPYFLKNSIKSINLLKKDKKILNYFLSKFLKKNIYLD
KTLSLINFDNLILEAKFSILKMWLYNNSQIIPTYYIIKRIYNELIDNENYFNKKILFQKKEIRRYKKNIYYINPEKKEKK
IVLYWKNIFQKIKLPNKLGYLIAHIKKKKKTKKNISYIPFPKKNTLITVCFHIKKKKTKIKKIWQKYNIPPWLRNNIPLL
FYNKKLISCIGIFIEKEKKKEKKKYISIKWINKIN
>Mature_435_residues
MFIKKLFKKYPTKKKFLLALSGGVDSTVLFYQLIIWKKKFQEINFRTIHINHHLHSHAKLAQKHCINICKKNKIEIKIFD
IIIPKKNKYGIEGYSRLERLKIFKKNILPEEILLTGHNMNDLCENIFLSLKRKSGINGISGMNFYSKYDNIKIIRPLINI
KKENILFWAKKKKIKWIEDQSNKNNQYDRNFIRNKILSKIYLKWPYFLKNSIKSINLLKKDKKILNYFLSKFLKKNIYLD
KTLSLINFDNLILEAKFSILKMWLYNNSQIIPTYYIIKRIYNELIDNENYFNKKILFQKKEIRRYKKNIYYINPEKKEKK
IVLYWKNIFQKIKLPNKLGYLIAHIKKKKKTKKNISYIPFPKKNTLITVCFHIKKKKTKIKKIWQKYNIPPWLRNNIPLL
FYNKKLISCIGIFIEKEKKKEKKKYISIKWINKIN

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=427, Percent_Identity=29.03981264637, Blast_Score=268, Evalue=6e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 52847; Mature: 52847

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIKKLFKKYPTKKKFLLALSGGVDSTVLFYQLIIWKKKFQEINFRTIHINHHLHSHAKL
CCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHH
AQKHCINICKKNKIEIKIFDIIIPKKNKYGIEGYSRLERLKIFKKNILPEEILLTGHNMN
HHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCCHH
DLCENIFLSLKRKSGINGISGMNFYSKYDNIKIIRPLINIKKENILFWAKKKKIKWIEDQ
HHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEHHHCCCCCCEEEEEECCCEEEECCC
SNKNNQYDRNFIRNKILSKIYLKWPYFLKNSIKSINLLKKDKKILNYFLSKFLKKNIYLD
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KTLSLINFDNLILEAKFSILKMWLYNNSQIIPTYYIIKRIYNELIDNENYFNKKILFQKK
HHHHHHCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
EIRRYKKNIYYINPEKKEKKIVLYWKNIFQKIKLPNKLGYLIAHIKKKKKTKKNISYIPF
HHHHHHCCEEEECCCCCCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
PKKNTLITVCFHIKKKKTKIKKIWQKYNIPPWLRNNIPLLFYNKKLISCIGIFIEKEKKK
CCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
EKKKYISIKWINKIN
HHHHEEEEEEEECCC
>Mature Secondary Structure
MFIKKLFKKYPTKKKFLLALSGGVDSTVLFYQLIIWKKKFQEINFRTIHINHHLHSHAKL
CCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHH
AQKHCINICKKNKIEIKIFDIIIPKKNKYGIEGYSRLERLKIFKKNILPEEILLTGHNMN
HHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCCHH
DLCENIFLSLKRKSGINGISGMNFYSKYDNIKIIRPLINIKKENILFWAKKKKIKWIEDQ
HHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEHHHCCCCCCEEEEEECCCEEEECCC
SNKNNQYDRNFIRNKILSKIYLKWPYFLKNSIKSINLLKKDKKILNYFLSKFLKKNIYLD
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KTLSLINFDNLILEAKFSILKMWLYNNSQIIPTYYIIKRIYNELIDNENYFNKKILFQKK
HHHHHHCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
EIRRYKKNIYYINPEKKEKKIVLYWKNIFQKIKLPNKLGYLIAHIKKKKKTKKNISYIPF
HHHHHHCCEEEECCCCCCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
PKKNTLITVCFHIKKKKTKIKKIWQKYNIPPWLRNNIPLLFYNKKLISCIGIFIEKEKKK
CCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
EKKKYISIKWINKIN
HHHHEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]