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The map label for this gene is hisF [H]

Identifier: 116515011

GI number: 116515011

Start: 75870

End: 76646

Strand: Direct

Name: hisF [H]

Synonym: BCc_069

Alternate gene names: 116515011

Gene position: 75870-76646 (Clockwise)

Preceding gene: 116515010

Following gene: 116515012

Centisome position: 18.22

GC content: 27.16

Gene sequence:

>777_bases
ATGTTGGCTAAAAGAATAATTGCATGTTTAGATGTTAAAAATGGAAAAGTGGTTAAAGGTATTAAATTTCGTAATCAAAT
TATTATAGGAGAAATTTTAGATTTAGCAGAATATTATTCTTTAAATGGTATTGATGAATTAGTTTTTTATGATATTTCAG
CGTCTCCTAAGCATGAATTATTAGATATTAAATGGATTTCTAAGATTGCTGAAATCATTAATATTCCTTTTTCAGTTGCT
GGAGGAATTTCTTCTGTTAAAGATGCTAAATTAATTTTATCATCAGGAGCAGATAAAATATCTATTAATTCTTCTGCATT
AAAATATCCTAGGTTAATTAGTGATTTAGTAGATCGATTTGGCTCTCAATGTATTGTAGTTGGAGTAGATTCATGGTTTA
ATATAGATAAAAAAAAATATCAAGTTTATCAATATACTGGAGATGAACATAAGTCTATTAAGACATCATGGGATACTCTA
GAATGGATAAGATATGTACAAAAATTAGGTGCTGGTGAAATCGTTGTTAATACTATGAATACTGATGGTACTAGAGAGGG
GTATGATATTATTCAATTGAAAAAAATACGAAGAATTTGTACTGTACCTTTAATTGCTTCAGGTGGTGCGGGAAAAATAG
ATGATTTTTTAAATGTTTTTCAGATTGCTAAAGTTGATGGTGCTTTAGCTGCTTCTGTTTTTCATAAAAATATTATTTCT
ATTTCAAATTTAAAAAATTTTTTGTTTAATCACGGAATTAGGGTAAGAGTATGTTAA

Upstream 100 bases:

>100_bases
CTATTTCTGATATATATAATTTAAAAAAAAATAATGTTGAACATGTTATTGTTGGTCGAGCGTTATTAGAAAACAAATTT
ACTTTTTTGGAGGCAAGTAA

Downstream 100 bases:

>100_bases
TTGATCAAAATTTACAAAATTTAAATTGGACAAAAAATAATGGTTTAATTCCTGCTATTGCGCAACATTATATTTCTGGA
GAAATATTGATGTTCGGTTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIACLDVKNGKVVKGIKFRNQIIIGEILDLAEYYSLNGIDELVFYDISASPKHELLDIKWISKIAEIINIPFSVA
GGISSVKDAKLILSSGADKISINSSALKYPRLISDLVDRFGSQCIVVGVDSWFNIDKKKYQVYQYTGDEHKSIKTSWDTL
EWIRYVQKLGAGEIVVNTMNTDGTREGYDIIQLKKIRRICTVPLIASGGAGKIDDFLNVFQIAKVDGALAASVFHKNIIS
ISNLKNFLFNHGIRVRVC

Sequences:

>Translated_258_residues
MLAKRIIACLDVKNGKVVKGIKFRNQIIIGEILDLAEYYSLNGIDELVFYDISASPKHELLDIKWISKIAEIINIPFSVA
GGISSVKDAKLILSSGADKISINSSALKYPRLISDLVDRFGSQCIVVGVDSWFNIDKKKYQVYQYTGDEHKSIKTSWDTL
EWIRYVQKLGAGEIVVNTMNTDGTREGYDIIQLKKIRRICTVPLIASGGAGKIDDFLNVFQIAKVDGALAASVFHKNIIS
ISNLKNFLFNHGIRVRVC
>Mature_258_residues
MLAKRIIACLDVKNGKVVKGIKFRNQIIIGEILDLAEYYSLNGIDELVFYDISASPKHELLDIKWISKIAEIINIPFSVA
GGISSVKDAKLILSSGADKISINSSALKYPRLISDLVDRFGSQCIVVGVDSWFNIDKKKYQVYQYTGDEHKSIKTSWDTL
EWIRYVQKLGAGEIVVNTMNTDGTREGYDIIQLKKIRRICTVPLIASGGAGKIDDFLNVFQIAKVDGALAASVFHKNIIS
ISNLKNFLFNHGIRVRVC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=60.8527131782946, Blast_Score=334, Evalue=4e-93,
Organism=Escherichia coli, GI87082028, Length=214, Percent_Identity=24.2990654205607, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319725, Length=321, Percent_Identity=28.6604361370716, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28758; Mature: 28758

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIACLDVKNGKVVKGIKFRNQIIIGEILDLAEYYSLNGIDELVFYDISASPKHEL
CCHHHHHHHEECCCCCEEEEEEECCEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHH
LDIKWISKIAEIINIPFSVAGGISSVKDAKLILSSGADKISINSSALKYPRLISDLVDRF
HHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHC
GSQCIVVGVDSWFNIDKKKYQVYQYTGDEHKSIKTSWDTLEWIRYVQKLGAGEIVVNTMN
CCCEEEEECCCCCCCCCCEEEEEEECCCCCCCHHCCHHHHHHHHHHHHCCCCEEEEEECC
TDGTREGYDIIQLKKIRRICTVPLIASGGAGKIDDFLNVFQIAKVDGALAASVFHKNIIS
CCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISNLKNFLFNHGIRVRVC
HHHHHHHHHHCCCEEEEC
>Mature Secondary Structure
MLAKRIIACLDVKNGKVVKGIKFRNQIIIGEILDLAEYYSLNGIDELVFYDISASPKHEL
CCHHHHHHHEECCCCCEEEEEEECCEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHH
LDIKWISKIAEIINIPFSVAGGISSVKDAKLILSSGADKISINSSALKYPRLISDLVDRF
HHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHC
GSQCIVVGVDSWFNIDKKKYQVYQYTGDEHKSIKTSWDTLEWIRYVQKLGAGEIVVNTMN
CCCEEEEECCCCCCCCCCEEEEEEECCCCCCCHHCCHHHHHHHHHHHHCCCCEEEEEECC
TDGTREGYDIIQLKKIRRICTVPLIASGGAGKIDDFLNVFQIAKVDGALAASVFHKNIIS
CCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISNLKNFLFNHGIRVRVC
HHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]