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The map label for this gene is dapE

Identifier: 116515001

GI number: 116515001

Start: 65594

End: 66724

Strand: Direct

Name: dapE

Synonym: BCc_059

Alternate gene names: 116515001

Gene position: 65594-66724 (Clockwise)

Preceding gene: 116515000

Following gene: 116515004

Centisome position: 15.75

GC content: 23.17

Gene sequence:

>1131_bases
ATGCATACTAATGTTTTAAATCTTTCAAAAAAATTAATTAATATATCTTCAATTAGTCCTCGAGATTTAGGTTGTCAAGA
GATTTTAATTAAACGTCTTCAATTATTGGGATTTTTTATAGAACGAATAAATTTAAAAGATACAAAAAATTTTTGGGCTT
ATAGAGGAAAGGGAAAGACAGTGACTTTTTTAGGTCATACTGATGTTGTTCCAGCTGGAAGTACTAGAAAGTGGAAAACT
TCACCATTTGTAGCAACTATAAAAGATGGTAAGTTATTTGGTAGAGGTTCAGCTGATATGAAGGGTTCTATTGCAGCTAT
GTTAATTGCAGTAGAAAATTTTATTAAAAAATTTCCGAATCATAAAGGTCGAATATCATTTTTAATAACTTCTGATGAAG
AAACTTCAGGAAAAAACGGAATTAGAAAAGTCGTTTCAATATTAAAAGAAAAAAAAGAAGTAATTGATTTTTGTTTAGTA
GGAGAACCTACTAGTGAAAAAATTTTAGGAGATTGTGTTAAAAATGGTCGACGAGGATCTCTTTCAGCAGATTTAATGAT
TTATGGTACTCAAGGACATATAGCCTATCCAAAATTATTTTTAAATCCTATTCATAATTCTATTCCATTTTTATTAGATT
TGTCTAATTTAATTTTTGATAAAGGTAATTTATTTTTTGAACCTACAAGTATTCAAATTTCTAAAATATTTTCTGAAAAA
AATTGTACATTAAATATGATTCCAGGAGAATTAAGAGTTTTTTTTAATATTCGTTTTAATACTTTAGTAAATAAAAAAAA
AATAATAAGAATTGTAGAAAATTTATTAAATAAATATTTAATAAAATATTCTATTTTTTGGACATATCATGCAAAACCAT
TTTTATCATCATCGAATTTTCTTTTAAATTTATTAACTAAATGTATATATAAACATACAAATATTATTCCAAAAATTAAA
AATAATGGTGGTACTTCTGATGCACGATTTATTTTTAATTTAACTGATAAAATAATAGAATTTGGTTTACCGAATTTAAC
TATTCATAAAGTTAATGAATATGTGTATATTAATGATTTATTAAAATTACAAAATATTTATTATAGTTTTTTAGAAAAGT
TATTATTATGA

Upstream 100 bases:

>100_bases
TTTTTAGTTAAAAATTTTTTATAAGTTCAATAAAATAAAAATAGAATTTCTTTATTTTAAATATTTAAATTATATATAAA
TAACATTAGGATATATATTT

Downstream 100 bases:

>100_bases
TCTTCTTTTAGAAGATATTTTTAGAATTGATATTTTCATGCAATGATAAATTTTTATCATTGCATAAAATATATTTTTTT
TAAAATAATAAATATTTTAT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MHTNVLNLSKKLINISSISPRDLGCQEILIKRLQLLGFFIERINLKDTKNFWAYRGKGKTVTFLGHTDVVPAGSTRKWKT
SPFVATIKDGKLFGRGSADMKGSIAAMLIAVENFIKKFPNHKGRISFLITSDEETSGKNGIRKVVSILKEKKEVIDFCLV
GEPTSEKILGDCVKNGRRGSLSADLMIYGTQGHIAYPKLFLNPIHNSIPFLLDLSNLIFDKGNLFFEPTSIQISKIFSEK
NCTLNMIPGELRVFFNIRFNTLVNKKKIIRIVENLLNKYLIKYSIFWTYHAKPFLSSSNFLLNLLTKCIYKHTNIIPKIK
NNGGTSDARFIFNLTDKIIEFGLPNLTIHKVNEYVYINDLLKLQNIYYSFLEKLLL

Sequences:

>Translated_376_residues
MHTNVLNLSKKLINISSISPRDLGCQEILIKRLQLLGFFIERINLKDTKNFWAYRGKGKTVTFLGHTDVVPAGSTRKWKT
SPFVATIKDGKLFGRGSADMKGSIAAMLIAVENFIKKFPNHKGRISFLITSDEETSGKNGIRKVVSILKEKKEVIDFCLV
GEPTSEKILGDCVKNGRRGSLSADLMIYGTQGHIAYPKLFLNPIHNSIPFLLDLSNLIFDKGNLFFEPTSIQISKIFSEK
NCTLNMIPGELRVFFNIRFNTLVNKKKIIRIVENLLNKYLIKYSIFWTYHAKPFLSSSNFLLNLLTKCIYKHTNIIPKIK
NNGGTSDARFIFNLTDKIIEFGLPNLTIHKVNEYVYINDLLKLQNIYYSFLEKLLL
>Mature_376_residues
MHTNVLNLSKKLINISSISPRDLGCQEILIKRLQLLGFFIERINLKDTKNFWAYRGKGKTVTFLGHTDVVPAGSTRKWKT
SPFVATIKDGKLFGRGSADMKGSIAAMLIAVENFIKKFPNHKGRISFLITSDEETSGKNGIRKVVSILKEKKEVIDFCLV
GEPTSEKILGDCVKNGRRGSLSADLMIYGTQGHIAYPKLFLNPIHNSIPFLLDLSNLIFDKGNLFFEPTSIQISKIFSEK
NCTLNMIPGELRVFFNIRFNTLVNKKKIIRIVENLLNKYLIKYSIFWTYHAKPFLSSSNFLLNLLTKCIYKHTNIIPKIK
NNGGTSDARFIFNLTDKIIEFGLPNLTIHKVNEYVYINDLLKLQNIYYSFLEKLLL

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=48, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1790395, Length=226, Percent_Identity=31.4159292035398, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17563146, Length=207, Percent_Identity=28.5024154589372, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_BUCCC (Q058B2)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802630.1
- ProteinModelPortal:   Q058B2
- SMR:   Q058B2
- STRING:   Q058B2
- EnsemblBacteria:   EBBUCT00000001402
- GeneID:   4440763
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_059
- eggNOG:   COG0624
- GeneTree:   EBGT00050000008078
- HOGENOM:   HBG728841
- OMA:   RIHAINE
- ProtClustDB:   PRK13009
- BioCyc:   BAPH372461:BCC_059-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 42867; Mature: 42867

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 68-68 ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTNVLNLSKKLINISSISPRDLGCQEILIKRLQLLGFFIERINLKDTKNFWAYRGKGKT
CCCCHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCE
VTFLGHTDVVPAGSTRKWKTSPFVATIKDGKLFGRGSADMKGSIAAMLIAVENFIKKFPN
EEEECCCCCCCCCCCCCEECCCEEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
HKGRISFLITSDEETSGKNGIRKVVSILKEKKEVIDFCLVGEPTSEKILGDCVKNGRRGS
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
LSADLMIYGTQGHIAYPKLFLNPIHNSIPFLLDLSNLIFDKGNLFFEPTSIQISKIFSEK
CEEEEEEEECCCCEECHHHHHHHHHCCCCCEEEHHHHHEECCCEEECCCCEEEEEHHCCC
NCTLNMIPGELRVFFNIRFNTLVNKKKIIRIVENLLNKYLIKYSIFWTYHAKPFLSSSNF
CCEEEECCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCHH
LLNLLTKCIYKHTNIIPKIKNNGGTSDARFIFNLTDKIIEFGLPNLTIHKVNEYVYINDL
HHHHHHHHHHHHCCCCCEECCCCCCCCCEEEEHHHHHHHHHCCCCEEEEECCCEEEHHHH
LKLQNIYYSFLEKLLL
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHTNVLNLSKKLINISSISPRDLGCQEILIKRLQLLGFFIERINLKDTKNFWAYRGKGKT
CCCCHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCE
VTFLGHTDVVPAGSTRKWKTSPFVATIKDGKLFGRGSADMKGSIAAMLIAVENFIKKFPN
EEEECCCCCCCCCCCCCEECCCEEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
HKGRISFLITSDEETSGKNGIRKVVSILKEKKEVIDFCLVGEPTSEKILGDCVKNGRRGS
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
LSADLMIYGTQGHIAYPKLFLNPIHNSIPFLLDLSNLIFDKGNLFFEPTSIQISKIFSEK
CEEEEEEEECCCCEECHHHHHHHHHCCCCCEEEHHHHHEECCCEEECCCCEEEEEHHCCC
NCTLNMIPGELRVFFNIRFNTLVNKKKIIRIVENLLNKYLIKYSIFWTYHAKPFLSSSNF
CCEEEECCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCHH
LLNLLTKCIYKHTNIIPKIKNNGGTSDARFIFNLTDKIIEFGLPNLTIHKVNEYVYINDL
HHHHHHHHHHHHCCCCCEECCCCCCCCCEEEEHHHHHHHHHCCCCEEEEECCCEEEHHHH
LKLQNIYYSFLEKLLL
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA