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The map label for this gene is dnaN [H]

Identifier: 116514953

GI number: 116514953

Start: 4535

End: 5641

Strand: Reverse

Name: dnaN [H]

Synonym: BCc_003

Alternate gene names: 116514953

Gene position: 5641-4535 (Counterclockwise)

Preceding gene: 116514954

Following gene: 116514952

Centisome position: 1.35

GC content: 15.72

Gene sequence:

>1107_bases
ATGGAATTTAAAATTAAAAAAAATGATTTTTTTAACTCATTTAAAAAAATGAATAGAATTATTACAAAAAATCCAATATT
TCCAATATTAGAAAACATAATTATTAAAATTAACGAAAAAAAATTAAAATTAACTAGTTCAAATTTAGAAGTAGAATTAA
ATGCGTATATAGATAAAAAATTTTTTATTTTATATAAATCTGGATGTGTTGCAATTTCTGGAAAAAAAATTTTAAATATT
TGTCGTAATATATCTAAAGATGCAATATTACATTTTAAAATTATAAATAAAAAAATGCGTATTTGTATACTAAATAGTTT
ATTTGAATTAAATACTATGATTTGTACTAATTTTCCTATTTTTAATGATTTTATAAAAAAAAAAGAATTTTTTATTTCTG
AAAAAATATTAAAAGAAATTATTTCATTAATATTTTTTTCAATAGCAAATAATGATATTAGAAATGTGTTAAATGGAATA
CTTATAGAATATAAAAAAAATAATTTATATGGTGTAACAACAGACGGATATCGATTATCAGTATACAAAATTGCAATAAA
TTTAAATATAAAAAATTTTTCTATTATTATTAATAAAAAAAGTATACTAGAATTATTTAGACTAATTAATTACACAAACA
AAAAAGTAAAAGTAATTATTAATAATAATTATGTTGCATTTAAAATAAATGAAATTTTATTAAAAACAAAAATATTAGAC
GGAAACTTTCCTAATTATAACGACGTAATTTTAAAAAAAAAAAAATATTCTATTTTAATACCTACAAAACCATTAAAAAA
ATCATTATCAAAAACATCAATATTATGCAATACTATATTTAAAGGTGTTTGTTTAAATTTTTTAAAAAATAAATTAATAA
TTACATCAAAAAATCAAGAAGACGAAAAATCATATGATTCATTTATTATTGATAATAAAAAAATAAAAATCTCTTTTCAT
ATAAATGTGTTTTATCTATTAGATATTCTTAATGTATTAGATGTTGAAGTTATTACTATATCATTTAGAATTCCTATTTC
TAGCATTCAAATTCAATCAGATAAAAAAAAAGAAATATTATATATAATAATGCCTTTAAAATTATAA

Upstream 100 bases:

>100_bases
TTTTATATGCTTATAAAAAAATAAATCAACTTAAAAAAAAAAAAACAGAAATTTATTCTGATTTTATATATCTCTTTAAC
CAATTAAATGCATAAAATTT

Downstream 100 bases:

>100_bases
TATAAAAATATTAAGTACATAAAAATATTATGTTTTAAATAAATATTAAATTTTAATTACCATATCTTAAAATTATTAAT
TTATAAAAATCTCTTTTTTA

Product: DNA polymerase III, b subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MEFKIKKNDFFNSFKKMNRIITKNPIFPILENIIIKINEKKLKLTSSNLEVELNAYIDKKFFILYKSGCVAISGKKILNI
CRNISKDAILHFKIINKKMRICILNSLFELNTMICTNFPIFNDFIKKKEFFISEKILKEIISLIFFSIANNDIRNVLNGI
LIEYKKNNLYGVTTDGYRLSVYKIAINLNIKNFSIIINKKSILELFRLINYTNKKVKVIINNNYVAFKINEILLKTKILD
GNFPNYNDVILKKKKYSILIPTKPLKKSLSKTSILCNTIFKGVCLNFLKNKLIITSKNQEDEKSYDSFIIDNKKIKISFH
INVFYLLDILNVLDVEVITISFRIPISSIQIQSDKKKEILYIIMPLKL

Sequences:

>Translated_368_residues
MEFKIKKNDFFNSFKKMNRIITKNPIFPILENIIIKINEKKLKLTSSNLEVELNAYIDKKFFILYKSGCVAISGKKILNI
CRNISKDAILHFKIINKKMRICILNSLFELNTMICTNFPIFNDFIKKKEFFISEKILKEIISLIFFSIANNDIRNVLNGI
LIEYKKNNLYGVTTDGYRLSVYKIAINLNIKNFSIIINKKSILELFRLINYTNKKVKVIINNNYVAFKINEILLKTKILD
GNFPNYNDVILKKKKYSILIPTKPLKKSLSKTSILCNTIFKGVCLNFLKNKLIITSKNQEDEKSYDSFIIDNKKIKISFH
INVFYLLDILNVLDVEVITISFRIPISSIQIQSDKKKEILYIIMPLKL
>Mature_368_residues
MEFKIKKNDFFNSFKKMNRIITKNPIFPILENIIIKINEKKLKLTSSNLEVELNAYIDKKFFILYKSGCVAISGKKILNI
CRNISKDAILHFKIINKKMRICILNSLFELNTMICTNFPIFNDFIKKKEFFISEKILKEIISLIFFSIANNDIRNVLNGI
LIEYKKNNLYGVTTDGYRLSVYKIAINLNIKNFSIIINKKSILELFRLINYTNKKVKVIINNNYVAFKINEILLKTKILD
GNFPNYNDVILKKKKYSILIPTKPLKKSLSKTSILCNTIFKGVCLNFLKNKLIITSKNQEDEKSYDSFIIDNKKIKISFH
INVFYLLDILNVLDVEVITISFRIPISSIQIQSDKKKEILYIIMPLKL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=368, Percent_Identity=27.445652173913, Blast_Score=217, Evalue=8e-58,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 43003; Mature: 43003

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKIKKNDFFNSFKKMNRIITKNPIFPILENIIIKINEKKLKLTSSNLEVELNAYIDKK
CCEEEECHHHHHHHHHHHHHHCCCCCHHHHHHEEEEECCCEEEEECCCEEEEEEEEECCE
FFILYKSGCVAISGKKILNICRNISKDAILHFKIINKKMRICILNSLFELNTMICTNFPI
EEEEEECCEEEECCHHHHHHHHCCCCCCEEEEEEECCCEEEEEHHHHHHHHHHEECCCCH
FNDFIKKKEFFISEKILKEIISLIFFSIANNDIRNVLNGILIEYKKNNLYGVTTDGYRLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEECCCEEEEECCCEEEE
VYKIAINLNIKNFSIIINKKSILELFRLINYTNKKVKVIINNNYVAFKINEILLKTKILD
EEEEEEEEEEEEEEEEEECHHHHHHHHHHCCCCCEEEEEEECCEEEEEEEEEEEEEEEEC
GNFPNYNDVILKKKKYSILIPTKPLKKSLSKTSILCNTIFKGVCLNFLKNKLIITSKNQE
CCCCCCCCEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
DEKSYDSFIIDNKKIKISFHINVFYLLDILNVLDVEVITISFRIPISSIQIQSDKKKEIL
CHHCCCCEEEECEEEEEEEEEEHHHHHHHHHHCCEEEEEEEEEECEEEEEECCCCCCEEE
YIIMPLKL
EEEEEECC
>Mature Secondary Structure
MEFKIKKNDFFNSFKKMNRIITKNPIFPILENIIIKINEKKLKLTSSNLEVELNAYIDKK
CCEEEECHHHHHHHHHHHHHHCCCCCHHHHHHEEEEECCCEEEEECCCEEEEEEEEECCE
FFILYKSGCVAISGKKILNICRNISKDAILHFKIINKKMRICILNSLFELNTMICTNFPI
EEEEEECCEEEECCHHHHHHHHCCCCCCEEEEEEECCCEEEEEHHHHHHHHHHEECCCCH
FNDFIKKKEFFISEKILKEIISLIFFSIANNDIRNVLNGILIEYKKNNLYGVTTDGYRLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEECCCEEEEECCCEEEE
VYKIAINLNIKNFSIIINKKSILELFRLINYTNKKVKVIINNNYVAFKINEILLKTKILD
EEEEEEEEEEEEEEEEEECHHHHHHHHHHCCCCCEEEEEEECCEEEEEEEEEEEEEEEEC
GNFPNYNDVILKKKKYSILIPTKPLKKSLSKTSILCNTIFKGVCLNFLKNKLIITSKNQE
CCCCCCCCEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
DEKSYDSFIIDNKKIKISFHINVFYLLDILNVLDVEVITISFRIPISSIQIQSDKKKEIL
CHHCCCCEEEECEEEEEEEEEEHHHHHHHHHHCCEEEEEEEEEECEEEEEECCCCCCEEE
YIIMPLKL
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11133977; 10993077 [H]