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The map label for this gene is adhE [H]

Identifier: 116494319

GI number: 116494319

Start: 769479

End: 772085

Strand: Direct

Name: adhE [H]

Synonym: LSEI_0775

Alternate gene names: 116494319

Gene position: 769479-772085 (Clockwise)

Preceding gene: 116494316

Following gene: 116494320

Centisome position: 26.58

GC content: 47.41

Gene sequence:

>2607_bases
ATGTTAAAGAATACTGCGAAGACTGCTACCGAAGTTGATGTAAAGAGCATGGTTGACGAACTGGTTGCGAATGCGCATGC
CGCTTTGAAGATCATGAAAACTTTCGATCAAGAAAAGATCGATCACATCGTTCATCGGATGGCAATCGCTGGTTTGGATC
ATCATATGGAATTGGCAAAACTCGCTGTTGACGAGACGGGCCGTGGCGTGTGGGAAGATAAAGCGATCAAGAACATGTTC
GCCACCGAAGAAATTTGGCACTCAATCAAGAACAACAAGACCGTTGGTGTGATCAACGAAGACAAACAACGCGGCTTGGT
ATCGATTGCTGAACCAATTGGTGTCATTGCTGGTGTGACGCCAGTGACGAACCCAACTTCAACGACCATGTTCAAATCCG
AAATTGCCATTAAAACCCGTAATCCAATCATTTTCGCTTTCCATCCGGGTGCCCAAAAGTCTTCAGCGCGTGCGTTGGAG
GTCATCCGTGATGAAGCCGAAAAAGCAGGCTTACCAAAGGGTGCTTTGCAATATATCCCTGTACCAAGTATGGATGCGAC
TAAAGCTTTGATGGATCACCCTGGTATCGCCACGATTTTGGCAACGGGCGGTCCTGGCATGGTTAAGTCCGCCTATTCAT
CAGGCAAGCCAGCACTGGGTGTTGGGGCCGGGAATGCGCCAGCTTACATTGAAGCCAGCGCTAACATCAAACAAGCTGTC
AATGACTTAGTACTTTCAAAGAGTTTTGATAATGGTATGATTTGTGCTTCCGAACAAGGCGTGATTATTGATTCCAGCAT
TTACGATGATGTGAAGAAGGAATTTGAAGCCCAAGGTGCTTACTTCGTTAAACAGAAGGACATGAAGAAGTTCGAAAGTA
CCGTCATTAATCTTGAAAAGCAAAGTGTCAATCCGCGAATCGTTGGTCAAAGTCCTAAGCAGATTGCTGAATGGGCGGGG
ATCACGATTCCTGATAACACCACGATTCTGATTGCTGAATTGAAAGGTGTTGGCGAAAAGTATCCACTGTCTCGTGAAAA
ACTAAGTCCAGTACTGGCCATGGTCAAAGCAGATGGTCATGAAGATGCCTTCAAGAAATGTGAAACCATGCTTGATATCG
GCGGTTTGGGTCATACGGCAGTCATTCACACGGCTGATGATGAGTTAGCTTTGAAGTATGCCGATGCTATGCAAGCTTGC
CGGATTTTGATCAACACACCATCTTCTGTCGGCGGGATTGGCGATCTTTACAACGAAATGATTCCAAGTTTGACACTGGG
CTGCGGCTCATACGGTGGTAACTCAATTTCGCATAACGTGGGGACGGTCGACTTGCTTAACATCAAGACAATGGCAAAAC
GCCGCAATAACATGCAGTGGATGAAATTGCCACCAAAGATCTATTTTGAAAAGAATTCTGTTCGTTACCTCGAACACATG
GAAGGCATCAAACGGGCCTTCATCGTTGCTGATCGAAGCATGGAAAAGTTAGGCTTCGTCAAGATCGTTGAAGATGTTCT
GGCTCGGCGTGAAAATCCTGTTCAAGTTCAGACTTTTGTCGATGTTGAACCTGATCCATCAACTGACACGGTCTTCAAGG
GGACCGATATCATGCGCTCATTTGGACCGGATACCGTCATTGCTATCGGCGGCGGGTCTGTTATGGATGCTGCCAAAGGG
ATGTGCTTGTTCAACGATGCCGGGGATGCTGATTTCTTCGGCGCTAAGCAGAAATTCTTGGATATCCGGAAGCGGACTTA
CACCTTCCCGAATCTGAATAAGACAAAGCTTGTCTGCATTCCAACAACTTCTGGGACTGGTTCAGAAGTAACACCTTTTG
CGGTTATCACCGATTCCAAGGCTGGCATCAAGTATCCGTTAGCTGACTATGCATTGACGCCAGACATCGCGATTGTGGAC
AGTCAGTTCATCGAATCCGTACCGCCAGTGGTTGTCGCTGATTCCGGTTTGGATGTGCTCTGCCACGCAACCGAAAGTTA
TGTTTCGACCATGGCCACTCATTACACGAAGGGCCTGAGTTTGGAAGCAATCAAGTTGGTCTTCGAAAATCTGGAAAAGA
GTTATCATGGCGACGTCGAAGCAAAGTCAAAGATGCATGATGCTTCAACCATTGCCGGGATGGCCTTTGCCAACGCACTG
TTGGGCATTAACCACAGCATTGCGCATAAGATTGGGCAAGCGTTCCATCTGCCTCACGGTCGTTGCATCGCGATCACGAT
GCCACATGTTATTCGCTTCAATGCGAGTCAACCGAAGAAGCGCGCTATCTGGGCGAAGTATAGCTACTTCCGCGCTAACG
AAGACTACGCAGAAATCGCTCGCTACCTTGGCCTTCCTGGCAAGACAACTGATGAATTGGTTGAAAGCTATGTCCAGGCC
TTCATCAAGCTGGCTCACAGTGTTGGCGTCAAGCTCAGTTTGAAGGAACAAGGCGTTAAAAAGGCTGATCTCGACAAGCA
AGTTGATCGTCTTGCCGAACTGGCTTATGAAGACAACTGCACCGTTACCAACCCGCAAGAACCGTTAATCGGCGACTTGA
AGCATATCATCCTTGATGAATATGAAGGCACCGAATCCACTTTCTAA

Upstream 100 bases:

>100_bases
AAAAATTCACCAAAATTGTTTACAAAATCACAAAGACCGGTTATGATGTTCTTGTGAAATGGTTAACAAAAAATTCCGCA
AAACACTTGGAGGTAAGCTC

Downstream 100 bases:

>100_bases
GATGTTTGCCGCTAATCATTTCACAATGATCGGCGCAGAGAATTCAATGGCGACATTATTTGAGCCGATCACAAAAAAAC
ACGTCAGTAAATTGGCGTGT

Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 868; Mature: 868

Protein sequence:

>868_residues
MLKNTAKTATEVDVKSMVDELVANAHAALKIMKTFDQEKIDHIVHRMAIAGLDHHMELAKLAVDETGRGVWEDKAIKNMF
ATEEIWHSIKNNKTVGVINEDKQRGLVSIAEPIGVIAGVTPVTNPTSTTMFKSEIAIKTRNPIIFAFHPGAQKSSARALE
VIRDEAEKAGLPKGALQYIPVPSMDATKALMDHPGIATILATGGPGMVKSAYSSGKPALGVGAGNAPAYIEASANIKQAV
NDLVLSKSFDNGMICASEQGVIIDSSIYDDVKKEFEAQGAYFVKQKDMKKFESTVINLEKQSVNPRIVGQSPKQIAEWAG
ITIPDNTTILIAELKGVGEKYPLSREKLSPVLAMVKADGHEDAFKKCETMLDIGGLGHTAVIHTADDELALKYADAMQAC
RILINTPSSVGGIGDLYNEMIPSLTLGCGSYGGNSISHNVGTVDLLNIKTMAKRRNNMQWMKLPPKIYFEKNSVRYLEHM
EGIKRAFIVADRSMEKLGFVKIVEDVLARRENPVQVQTFVDVEPDPSTDTVFKGTDIMRSFGPDTVIAIGGGSVMDAAKG
MCLFNDAGDADFFGAKQKFLDIRKRTYTFPNLNKTKLVCIPTTSGTGSEVTPFAVITDSKAGIKYPLADYALTPDIAIVD
SQFIESVPPVVVADSGLDVLCHATESYVSTMATHYTKGLSLEAIKLVFENLEKSYHGDVEAKSKMHDASTIAGMAFANAL
LGINHSIAHKIGQAFHLPHGRCIAITMPHVIRFNASQPKKRAIWAKYSYFRANEDYAEIARYLGLPGKTTDELVESYVQA
FIKLAHSVGVKLSLKEQGVKKADLDKQVDRLAELAYEDNCTVTNPQEPLIGDLKHIILDEYEGTESTF

Sequences:

>Translated_868_residues
MLKNTAKTATEVDVKSMVDELVANAHAALKIMKTFDQEKIDHIVHRMAIAGLDHHMELAKLAVDETGRGVWEDKAIKNMF
ATEEIWHSIKNNKTVGVINEDKQRGLVSIAEPIGVIAGVTPVTNPTSTTMFKSEIAIKTRNPIIFAFHPGAQKSSARALE
VIRDEAEKAGLPKGALQYIPVPSMDATKALMDHPGIATILATGGPGMVKSAYSSGKPALGVGAGNAPAYIEASANIKQAV
NDLVLSKSFDNGMICASEQGVIIDSSIYDDVKKEFEAQGAYFVKQKDMKKFESTVINLEKQSVNPRIVGQSPKQIAEWAG
ITIPDNTTILIAELKGVGEKYPLSREKLSPVLAMVKADGHEDAFKKCETMLDIGGLGHTAVIHTADDELALKYADAMQAC
RILINTPSSVGGIGDLYNEMIPSLTLGCGSYGGNSISHNVGTVDLLNIKTMAKRRNNMQWMKLPPKIYFEKNSVRYLEHM
EGIKRAFIVADRSMEKLGFVKIVEDVLARRENPVQVQTFVDVEPDPSTDTVFKGTDIMRSFGPDTVIAIGGGSVMDAAKG
MCLFNDAGDADFFGAKQKFLDIRKRTYTFPNLNKTKLVCIPTTSGTGSEVTPFAVITDSKAGIKYPLADYALTPDIAIVD
SQFIESVPPVVVADSGLDVLCHATESYVSTMATHYTKGLSLEAIKLVFENLEKSYHGDVEAKSKMHDASTIAGMAFANAL
LGINHSIAHKIGQAFHLPHGRCIAITMPHVIRFNASQPKKRAIWAKYSYFRANEDYAEIARYLGLPGKTTDELVESYVQA
FIKLAHSVGVKLSLKEQGVKKADLDKQVDRLAELAYEDNCTVTNPQEPLIGDLKHIILDEYEGTESTF
>Mature_868_residues
MLKNTAKTATEVDVKSMVDELVANAHAALKIMKTFDQEKIDHIVHRMAIAGLDHHMELAKLAVDETGRGVWEDKAIKNMF
ATEEIWHSIKNNKTVGVINEDKQRGLVSIAEPIGVIAGVTPVTNPTSTTMFKSEIAIKTRNPIIFAFHPGAQKSSARALE
VIRDEAEKAGLPKGALQYIPVPSMDATKALMDHPGIATILATGGPGMVKSAYSSGKPALGVGAGNAPAYIEASANIKQAV
NDLVLSKSFDNGMICASEQGVIIDSSIYDDVKKEFEAQGAYFVKQKDMKKFESTVINLEKQSVNPRIVGQSPKQIAEWAG
ITIPDNTTILIAELKGVGEKYPLSREKLSPVLAMVKADGHEDAFKKCETMLDIGGLGHTAVIHTADDELALKYADAMQAC
RILINTPSSVGGIGDLYNEMIPSLTLGCGSYGGNSISHNVGTVDLLNIKTMAKRRNNMQWMKLPPKIYFEKNSVRYLEHM
EGIKRAFIVADRSMEKLGFVKIVEDVLARRENPVQVQTFVDVEPDPSTDTVFKGTDIMRSFGPDTVIAIGGGSVMDAAKG
MCLFNDAGDADFFGAKQKFLDIRKRTYTFPNLNKTKLVCIPTTSGTGSEVTPFAVITDSKAGIKYPLADYALTPDIAIVD
SQFIESVPPVVVADSGLDVLCHATESYVSTMATHYTKGLSLEAIKLVFENLEKSYHGDVEAKSKMHDASTIAGMAFANAL
LGINHSIAHKIGQAFHLPHGRCIAITMPHVIRFNASQPKKRAIWAKYSYFRANEDYAEIARYLGLPGKTTDELVESYVQA
FIKLAHSVGVKLSLKEQGVKKADLDKQVDRLAELAYEDNCTVTNPQEPLIGDLKHIILDEYEGTESTF

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=318, Percent_Identity=28.3018867924528, Blast_Score=100, Evalue=9e-21,
Organism=Escherichia coli, GI1787493, Length=862, Percent_Identity=47.3317865429234, Blast_Score=760, Evalue=0.0,
Organism=Escherichia coli, GI1789163, Length=399, Percent_Identity=31.077694235589, Blast_Score=185, Evalue=1e-47,
Organism=Escherichia coli, GI48994951, Length=368, Percent_Identity=33.695652173913, Blast_Score=177, Evalue=3e-45,
Organism=Escherichia coli, GI87082107, Length=398, Percent_Identity=31.9095477386935, Blast_Score=169, Evalue=7e-43,
Organism=Escherichia coli, GI1788797, Length=378, Percent_Identity=31.2169312169312, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1789386, Length=391, Percent_Identity=22.5063938618926, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17537053, Length=271, Percent_Identity=27.3062730627306, Blast_Score=96, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6321181, Length=418, Percent_Identity=31.1004784688995, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324950, Length=313, Percent_Identity=23.6421725239617, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24657991, Length=386, Percent_Identity=28.4974093264249, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 94546; Mature: 94546

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNTAKTATEVDVKSMVDELVANAHAALKIMKTFDQEKIDHIVHRMAIAGLDHHMELAK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHH
LAVDETGRGVWEDKAIKNMFATEEIWHSIKNNKTVGVINEDKQRGLVSIAEPIGVIAGVT
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
PVTNPTSTTMFKSEIAIKTRNPIIFAFHPGAQKSSARALEVIRDEAEKAGLPKGALQYIP
CCCCCCCCCEEEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECC
VPSMDATKALMDHPGIATILATGGPGMVKSAYSSGKPALGVGAGNAPAYIEASANIKQAV
CCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCEEEECCCCCCEEEEECCCHHHHH
NDLVLSKSFDNGMICASEQGVIIDSSIYDDVKKEFEAQGAYFVKQKDMKKFESTVINLEK
HHHHHHCCCCCCEEEECCCCEEEEHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHEEHHH
QSVNPRIVGQSPKQIAEWAGITIPDNTTILIAELKGVGEKYPLSREKLSPVLAMVKADGH
HCCCCEEECCCHHHHHHHCCEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
EDAFKKCETMLDIGGLGHTAVIHTADDELALKYADAMQACRILINTPSSVGGIGDLYNEM
HHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHH
IPSLTLGCGSYGGNSISHNVGTVDLLNIKTMAKRRNNMQWMKLPPKIYFEKNSVRYLEHM
CCCEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCEEEECCCEEEEECCCHHHHHHH
EGIKRAFIVADRSMEKLGFVKIVEDVLARRENPVQVQTFVDVEPDPSTDTVFKGTDIMRS
HHHHHEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEECCHHHHHH
FGPDTVIAIGGGSVMDAAKGMCLFNDAGDADFFGAKQKFLDIRKRTYTFPNLNKTKLVCI
CCCCEEEEECCCHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
PTTSGTGSEVTPFAVITDSKAGIKYPLADYALTPDIAIVDSQFIESVPPVVVADSGLDVL
ECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHCCCEEEECCCCEEE
CHATESYVSTMATHYTKGLSLEAIKLVFENLEKSYHGDVEAKSKMHDASTIAGMAFANAL
EECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LGINHSIAHKIGQAFHLPHGRCIAITMPHVIRFNASQPKKRAIWAKYSYFRANEDYAEIA
HCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCHHHHHEEEHHEEECCCCHHHHH
RYLGLPGKTTDELVESYVQAFIKLAHSVGVKLSLKEQGVKKADLDKQVDRLAELAYEDNC
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCHHHHHHHHHHHHHHHHCCCC
TVTNPQEPLIGDLKHIILDEYEGTESTF
CCCCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLKNTAKTATEVDVKSMVDELVANAHAALKIMKTFDQEKIDHIVHRMAIAGLDHHMELAK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHH
LAVDETGRGVWEDKAIKNMFATEEIWHSIKNNKTVGVINEDKQRGLVSIAEPIGVIAGVT
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
PVTNPTSTTMFKSEIAIKTRNPIIFAFHPGAQKSSARALEVIRDEAEKAGLPKGALQYIP
CCCCCCCCCEEEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECC
VPSMDATKALMDHPGIATILATGGPGMVKSAYSSGKPALGVGAGNAPAYIEASANIKQAV
CCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCEEEECCCCCCEEEEECCCHHHHH
NDLVLSKSFDNGMICASEQGVIIDSSIYDDVKKEFEAQGAYFVKQKDMKKFESTVINLEK
HHHHHHCCCCCCEEEECCCCEEEEHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHEEHHH
QSVNPRIVGQSPKQIAEWAGITIPDNTTILIAELKGVGEKYPLSREKLSPVLAMVKADGH
HCCCCEEECCCHHHHHHHCCEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
EDAFKKCETMLDIGGLGHTAVIHTADDELALKYADAMQACRILINTPSSVGGIGDLYNEM
HHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHH
IPSLTLGCGSYGGNSISHNVGTVDLLNIKTMAKRRNNMQWMKLPPKIYFEKNSVRYLEHM
CCCEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCEEEECCCEEEEECCCHHHHHHH
EGIKRAFIVADRSMEKLGFVKIVEDVLARRENPVQVQTFVDVEPDPSTDTVFKGTDIMRS
HHHHHEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEECCHHHHHH
FGPDTVIAIGGGSVMDAAKGMCLFNDAGDADFFGAKQKFLDIRKRTYTFPNLNKTKLVCI
CCCCEEEEECCCHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
PTTSGTGSEVTPFAVITDSKAGIKYPLADYALTPDIAIVDSQFIESVPPVVVADSGLDVL
ECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHCCCEEEECCCCEEE
CHATESYVSTMATHYTKGLSLEAIKLVFENLEKSYHGDVEAKSKMHDASTIAGMAFANAL
EECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LGINHSIAHKIGQAFHLPHGRCIAITMPHVIRFNASQPKKRAIWAKYSYFRANEDYAEIA
HCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCHHHHHEEEHHEEECCCCHHHHH
RYLGLPGKTTDELVESYVQAFIKLAHSVGVKLSLKEQGVKKADLDKQVDRLAELAYEDNC
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCHHHHHHHHHHHHHHHHCCCC
TVTNPQEPLIGDLKHIILDEYEGTESTF
CCCCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]